Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma

Jia,Bo; Qiu,Xiaoling; Chu,Hongxing; Sun,Xiang; Xu,Shuaimei; Zhao,Xinyuan; Zhao,Jianjiang

doi:10.3892/or.2019.6974

Wnt7a predicts poor prognosis, and contributes to growth and metastasis in tongue squamous cell carcinoma

Authors:
- Bo Jia
- Xiaoling Qiu
- Hongxing Chu
- Xiang Sun
- Shuaimei Xu
- Xinyuan Zhao
- Jianjiang Zhao
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Affiliations: Department of Oral Surgery, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
Published online on: January 22, 2019 https://doi.org/10.3892/or.2019.6974
Pages: 1749-1758

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Abstract

Regional and distant metastases are the principal reasons underlying the high mortality rate associated with tongue squamous cell carcinoma (TSCC); however, the precise molecular mechanisms involved in tongue tumorigenesis remain unknown. The present study aimed to determine the expression and mechanism of regulation of Wnt7a in the growth and metastasis of TSCC. Wnt7a mRNA and protein expression levels were examined in TSCC tissues using reverse transcription‑quantitative polymerase chain reaction and immunohistochemical staining. A loss‑of‑function assay was performed in TSCC cell lines using Wnt7a small interfering RNA or short hairpin RNA, after which, cell proliferation, migration and invasion were analyzed using Cell Counting Kit‑8, tumorigenicity and Transwell assays, respectively. Epithelial‑mesenchymal transition (EMT)‑associated proteins were detected by western blotting. The mRNA and protein expression levels of Wnt7a were significantly upregulated in cancer tissues compared with in the adjacent non‑cancerous tissues. Clinical analysis indicated that Wnt7a expression was associated with T classification, lymph node metastasis and pathological differentiation, and high Wnt7a expression predicted a short recurrence‑free survival for patients with TSCC. Silencing Wnt7a expression suppressed cell proliferation, migration and invasion, and reversed the EMT phenotype in TSCC cell lines. The present study revealed that Wnt7a may be upregulated in TSCC, where it may participate in modulating cell proliferation, migration, invasion and the EMT of TSCC. Therefore, Wnt7a should be considered a novel oncogene, and a potential prognostic and therapeutic target for patients with TSCC.

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