Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells

  • Authors:
    • Minoru Tomizawa
    • Fuminobu Shinozaki
    • Takao Sugiyama
    • Shigenori Yamamoto
    • Makoto Sueishi
    • Takanobu Yoshida
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  • Published online on: August 13, 2012     https://doi.org/10.3892/br.2012.2
  • Pages: 18-22
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Abstract

Induced pluripotent stem (iPS) cells are ideal sources of hepatocyte for transplantation into patients experiencing hepatic failure. Growth and transcription factors were analyzed to design a single-step protocol for the differentiation of iPS cells into hepatocytes. The expression of transcription factors was analyzed using reverse transcription-polymerase chain reaction (RT-PCR) and compared among iPS cells, as well as fetal and adult liver cells. iPS cells were cultured with growth factors and RT-PCR was performed to analyze the expression of transcription factors. iPS cells were introduced with transcription factors, cultured with growth factors and subjected to real-time quantitative PCR. Indocyanine green (ICG) was added to the medium as a hepatocyte marker. Sox17, GATA4, GATA6, FoxA2, HEX, HNF4α and C/EBPα were expressed in fetal and adult liver cells, but not in iPS cells. Sox17, GATA6 and HNF4α were expressed after exposure a combination of oncostatin M, epidermal growth factor, retinoic acid, dexamethasone and ITS (OERDITS). When iPS cells were introduced with FoxA2, GATA4, HEX and C/EBPα and cultured with OERDITS for 8 days, the cells expressed α-fetoprotein, δ-like (Dlk)-1 and γ-glutamyl transpeptidase (GTP), and ICG uptake was observed. Exposure to FoxA2, GATA4, HEX and C/EBPα and culturing with OERDITS supplementation potentially serves as a single-step inducer for the differentiation of iPS cells into hepatic progenitor-like cells within 8 days.
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January-February 2013
Volume 1 Issue 1

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Spandidos Publications style
Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M and Yoshida T: Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells. Biomed Rep 1: 18-22, 2013
APA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., & Yoshida, T. (2013). Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells. Biomedical Reports, 1, 18-22. https://doi.org/10.3892/br.2012.2
MLA
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells". Biomedical Reports 1.1 (2013): 18-22.
Chicago
Tomizawa, M., Shinozaki, F., Sugiyama, T., Yamamoto, S., Sueishi, M., Yoshida, T."Single-step protocol for the differentiation of human-induced pluripotent stem cells into hepatic progenitor-like cells". Biomedical Reports 1, no. 1 (2013): 18-22. https://doi.org/10.3892/br.2012.2