Uptake and efflux of 3-O-methyl-D-glucose in rat parotid cells

Jurysta,Cedric; Louchami,Karim; Malaisse,Willy  J.; Sener,Abdullah

doi:10.3892/br.2013.102

July-August 2013 Volume 1 Issue 4

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July-August 2013 Volume 1 Issue 4

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Article

Uptake and efflux of 3-O-methyl-D-glucose in rat parotid cells

Authors:
- Cedric Jurysta
- Karim Louchami
- Willy J. Malaisse
- Abdullah Sener
View Affiliations / Copyright

Affiliations: Laboratory of Experimental Hormonology, Brussels Free University (ULB), B-1070 Brussels, Belgium
Pages: 638-640
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Published online on: May 9, 2013

https://doi.org/10.3892/br.2013.102
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Abstract

In the framework of recent investigations on the regulation of D-glucose production by salivary glands, the aim of the present study was to compare the uptake of 3-O-[14C]methyl-D-glucose by rat parotid cells over a 6‑min incubation period at 37˚C to its efflux from prelabelled parotid cells, also incubated for 6 min at 37˚C. It was first assessed that the intracellular 3HOH water space, whether expressed in absolute terms or relative to the total 3HOH distribution space, is not significantly different between parotid cells obtained from either control rats or streptozotocin-induced diabetic rats. In the control rats, the uptake of 3-O-[14C]methyl-D-glucose corresponded, following correction for extracellular contamination, to a mean distribution space of 0.44±0.05 nl/103 cells, representing 29.8±3.4% of the intracellular water space. The efflux of 3-O-[14C]methyl-D-glucose from prelabelled parotid cells, expressed relative to their initial radioactive content, averaged 82.9±4.8 and 84.1±2.5% in control and diabetic rats, respectively. These findings suggest that the increased production of salivary D-glucose in diabetic subjects may be attributable to hyperglycemia, rather than to any major perturbation of the intrinsic processes involved, at least in parotid cells, in hexose handling.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Uptake and efflux of 3-O-methyl-D-glucose in rat parotid cells

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