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Article

Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease

  • Authors:
    • Ajda Çoker‑Gürkan
    • Serdar Arisan
    • Elif Damla Arisan
    • Narçin Palavan Ünsal
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Genetics, Science and Letter Faculty, Istanbul Kultur University, Atakoy Campus, 34156 Istanbul, Turkey, 1st Urology Clinics, Şişli Etfal Research and Training Hospital, Sisli, 34377 Istanbul, Turkey
  • Pages: 69-74
    |
    Published online on: October 21, 2013
       https://doi.org/10.3892/br.2013.184
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Abstract

Urolithiasis is a complex and multifactorial disorder characterized by the presence of stones in the urinary tract. Urea cycle is an important process involved in disease progression. L‑ornithine is a key amino acid in the urea cycle and is converted to putrescine by ornithine decarboxylase (ODC). Putrescine, spermidine and spermine are natural polyamines that are catabolized by a specific enzyme, spermidine/spermine acetyltransferase (SSAT). The single‑nucleotide polymorphisms (SNPs) in the intron region of ODC (+316 G>A) and promoter region of SSAT (‑1415 T>C) genes have been found to be associated with the polyamines expression levels. The aim of this study was to examine whether the ODC (+316 G>A) intron 1 region gene polymorphism and SAT‑1 promoter region (‑1415 T>C) gene polymorphisms are potential genetic markers for susceptibility to urolithiasis. A control group of 104 healthy subjects and a group of 65 patients with recurrent idiopathic calcium oxalate stone disease were enrolled into this study. Polymerase chain reaction (PCR)‑based restriction analysis was performed for the ODC intron 1 (+316 G>A) region and SAT‑1 (‑1415 T>C) promoter gene polymorphisms by PstI and MspI restriction enzyme digestion, respectively. The genotype distribution of polymorphisms studied in the ODC intron 1 region (+316 G>A) and SAT‑1 ‑1415 T>C promoter region did not reveal a significant difference between urolithiasis and the control groups (P=0.713 and 0.853), respectively. Furthermore, no significant difference was observed between the control and patient groups for ODC +316 G>A and SAT‑1 ‑1415 T>C allele frequencies (P=0.877 and 0.644), respectively. In conclusion, results of the present study suggest that ODC + 316 G>A and SAT‑1 ‑1415 T>C gene polymorphisms might not be a risk factor for urolithiasis.
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Copy and paste a formatted citation
Spandidos Publications style
Çoker‑Gürkan A, Arisan S, Arisan ED and Ünsal NP: Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease. Biomed Rep 2: 69-74, 2014.
APA
Çoker‑Gürkan, A., Arisan, S., Arisan, E.D., & Ünsal, N.P. (2014). Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease. Biomedical Reports, 2, 69-74. https://doi.org/10.3892/br.2013.184
MLA
Çoker‑Gürkan, A., Arisan, S., Arisan, E. D., Ünsal, N. P."Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease". Biomedical Reports 2.1 (2014): 69-74.
Chicago
Çoker‑Gürkan, A., Arisan, S., Arisan, E. D., Ünsal, N. P."Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease". Biomedical Reports 2, no. 1 (2014): 69-74. https://doi.org/10.3892/br.2013.184
Copy and paste a formatted citation
x
Spandidos Publications style
Çoker‑Gürkan A, Arisan S, Arisan ED and Ünsal NP: Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease. Biomed Rep 2: 69-74, 2014.
APA
Çoker‑Gürkan, A., Arisan, S., Arisan, E.D., & Ünsal, N.P. (2014). Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease. Biomedical Reports, 2, 69-74. https://doi.org/10.3892/br.2013.184
MLA
Çoker‑Gürkan, A., Arisan, S., Arisan, E. D., Ünsal, N. P."Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease". Biomedical Reports 2.1 (2014): 69-74.
Chicago
Çoker‑Gürkan, A., Arisan, S., Arisan, E. D., Ünsal, N. P."Lack of evidence for the association of ornithine decarboxylase (+316 G>A), spermidine/spermine acetyl transferase (‑1415 T>C) gene polymorphisms with calcium oxalate stone disease". Biomedical Reports 2, no. 1 (2014): 69-74. https://doi.org/10.3892/br.2013.184
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