An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18

  • Authors:
    • Shu Gong
    • Zhonghua Tao
    • Xiaoyan Liu
    • Lin Gan
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  • Published online on: November 6, 2013     https://doi.org/10.3892/br.2013.197
  • Pages: 85-88
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Abstract

Recent studies have reported the association between the expression of oncoprotein 18 (op18) and hepato­cellular carcinoma (HCC). However, any underlying mechanistic connection between op18 expression and hepatocarcino­genesis is poorly understood. In the present study, Flag-pcDNA3.1 vector and Flag-pcDNA3.1-op18 plasmid were stably transfected in SMMC7721 cells, respectively. Stable SMMC7721 control and op18 overexpression SMMC7721 cell lines were constructed and identified by western blot analysis. Using a cell counting kit-8 (CCK8), it was shown that cell proliferation was significantly increased in the op18 overexpression SMMC7721 cell group (0.60±0.05), compared with the control group (0.29±0.03) at an absorbance of 450 nm (P<0.01). Flow cytometry was used to analyze cell apoptosis by FITC‑Annexin Ⅴ and propidium iodide (PI) apoptosis assay kit. The results demonstrated that the percentage of apoptotic cells was inhibited to 5.80±0.33% in the op18 overexpression group, compared with 11.79±1.09% in the control group. Using FACS, single cell analysis data showed that op18 overexpression induced cell cycle arrest by inhibiting progression from G2 to M phase. The results suggest that op18 expression is closely associated with SMMC7721 cell proliferation and apoptosis, which appears to be a potential predictor of prognosis in HCC.
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January-February 2014
Volume 2 Issue 1

Print ISSN: 2049-9434
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Spandidos Publications style
Gong S, Tao Z, Liu X and Gan L: An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18. Biomed Rep 2: 85-88, 2014
APA
Gong, S., Tao, Z., Liu, X., & Gan, L. (2014). An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18. Biomedical Reports, 2, 85-88. https://doi.org/10.3892/br.2013.197
MLA
Gong, S., Tao, Z., Liu, X., Gan, L."An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18". Biomedical Reports 2.1 (2014): 85-88.
Chicago
Gong, S., Tao, Z., Liu, X., Gan, L."An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18". Biomedical Reports 2, no. 1 (2014): 85-88. https://doi.org/10.3892/br.2013.197