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Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK

  • Authors:
    • Noriko Yokoyama
    • W. Todd Miller
  • View Affiliations / Copyright

    Affiliations: Department of Physiology and Biophysics, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
  • Pages: 9-13
    |
    Published online on: November 3, 2014
       https://doi.org/10.3892/br.2014.374
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Abstract

Anaplastic lymphoma kinase (ALK) is a member of the receptor tyrosine kinase superfamily. The ALK gene is a site of frequent mutation and chromosomal rearrangement in various types of human cancers. A novel chromosomal translocation was recently identified in human colorectal cancer between the ALK gene and chromosome 2, open reading frame 44 (C2orf44), a gene of unknown function. As a first step in understanding the oncogenic properties of this fusion protein, C2orf44 cDNA was cloned and the encoded protein was characterized, which was designated as WD repeat and coiled coil containing protein (WDCP). A C‑terminal proline‑rich segment in WDCP was shown to mediate binding to the Src homology 3 domain of the Src family kinase hematopoietic cell kinase (Hck). Co‑expression with Hck lead to tyrosine phosphorylation of WDCP. Chromatographic fractionation of WDCP‑containing lysates indicates that the protein exists as an oligomer in mammalian cells. These results suggest that, in the context of the ALK‑C2orf44 gene fusion, WDCP imposes an oligomeric structure on ALK that results in constitutive kinase activation and signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Yokoyama N and Miller WT: Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK. Biomed Rep 3: 9-13, 2015.
APA
Yokoyama, N., & Miller, W.T. (2015). Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK. Biomedical Reports, 3, 9-13. https://doi.org/10.3892/br.2014.374
MLA
Yokoyama, N., Miller, W. T."Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK". Biomedical Reports 3.1 (2015): 9-13.
Chicago
Yokoyama, N., Miller, W. T."Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK". Biomedical Reports 3, no. 1 (2015): 9-13. https://doi.org/10.3892/br.2014.374
Copy and paste a formatted citation
x
Spandidos Publications style
Yokoyama N and Miller WT: Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK. Biomed Rep 3: 9-13, 2015.
APA
Yokoyama, N., & Miller, W.T. (2015). Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK. Biomedical Reports, 3, 9-13. https://doi.org/10.3892/br.2014.374
MLA
Yokoyama, N., Miller, W. T."Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK". Biomedical Reports 3.1 (2015): 9-13.
Chicago
Yokoyama, N., Miller, W. T."Molecular characterization of WDCP, a novel fusion partner for the anaplastic lymphoma tyrosine kinase ALK". Biomedical Reports 3, no. 1 (2015): 9-13. https://doi.org/10.3892/br.2014.374
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