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Article

Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet

  • Authors:
    • Yongshou Yang
    • Novita Vivi Sitanggang
    • Norihisa Kato
    • Junji Inoue
    • Takayuki Murakami
    • Toshiro Watanabe
    • Takafumi Iguchi
    • Yukako Okazaki
  • View Affiliations / Copyright

    Affiliations: Graduate School of Biosphere Science, Hiroshima University, Higashi‑Hiroshima 739‑8528, Japan, Ahjikan Co., Ltd., Hiroshima 733‑8677, Japan, Technology Development Laboratory, Yaegaki Bio‑Industry Incorporated, Himeji 679‑4298, Japan, Faculty of Human Life Sciences, Fuji Women's University, Ishikari, Hokkaido 061‑3204, Japan
  • Pages: 715-720
    |
    Published online on: July 15, 2015
       https://doi.org/10.3892/br.2015.490
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Abstract

The present study investigated the effects of the dietary addition of the protease preparations derived from Aspergillus on the colonic luminal environment. Rats were fed a 30% beef tallow diet with or without the protease preparations, including Amano protease (protease A ‘Amano SD’, neutral proteases from Aspergillus spp.) or orientase (orientase AY, acid proteases from Aspergillus niger) at the dose of 0.2% for 3 weeks. Cecal Bifidobacterium was significantly elevated in the dietary Amano protease group (194‑fold, P<0.05), but not in the orientase group. Lactobacillus was elevated in the two groups (P<0.05). Cecal n‑butyrate, propionate and lactate were higher in the Amano protease and orientase groups compared with the controls (P<0.05). Fecal immunoglobulin A and mucins were elevated in the Amano protease group (P<0.05). These results suggest the potential effect of the consumption of Aspergillus‑derived protease preparations that are favorable for the colonic luminal environment in rats fed a high-fat diet.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Sitanggang NV, Kato N, Inoue J, Murakami T, Watanabe T, Iguchi T and Okazaki Y: Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet. Biomed Rep 3: 715-720, 2015.
APA
Yang, Y., Sitanggang, N.V., Kato, N., Inoue, J., Murakami, T., Watanabe, T. ... Okazaki, Y. (2015). Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet. Biomedical Reports, 3, 715-720. https://doi.org/10.3892/br.2015.490
MLA
Yang, Y., Sitanggang, N. V., Kato, N., Inoue, J., Murakami, T., Watanabe, T., Iguchi, T., Okazaki, Y."Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet". Biomedical Reports 3.5 (2015): 715-720.
Chicago
Yang, Y., Sitanggang, N. V., Kato, N., Inoue, J., Murakami, T., Watanabe, T., Iguchi, T., Okazaki, Y."Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet". Biomedical Reports 3, no. 5 (2015): 715-720. https://doi.org/10.3892/br.2015.490
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Sitanggang NV, Kato N, Inoue J, Murakami T, Watanabe T, Iguchi T and Okazaki Y: Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet. Biomed Rep 3: 715-720, 2015.
APA
Yang, Y., Sitanggang, N.V., Kato, N., Inoue, J., Murakami, T., Watanabe, T. ... Okazaki, Y. (2015). Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet. Biomedical Reports, 3, 715-720. https://doi.org/10.3892/br.2015.490
MLA
Yang, Y., Sitanggang, N. V., Kato, N., Inoue, J., Murakami, T., Watanabe, T., Iguchi, T., Okazaki, Y."Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet". Biomedical Reports 3.5 (2015): 715-720.
Chicago
Yang, Y., Sitanggang, N. V., Kato, N., Inoue, J., Murakami, T., Watanabe, T., Iguchi, T., Okazaki, Y."Beneficial effects of protease preparations derived from Aspergillus on the colonic luminal environment in rats consuming a high-fat diet". Biomedical Reports 3, no. 5 (2015): 715-720. https://doi.org/10.3892/br.2015.490
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