Topiramate effects lipolysis in 3T3-L1 adipocytes

Martins,Gabriela Poltronieri Campagnaro; Souza,Camila Oliveira; Marques,Scherolin de Oliveira; Luciano,Thais Fernandes; da Silva Pieri,Bruno Luiz; Rosa,José César; da Silva,Adelino Sanchez Ramos; Pauli,José Rodrigo; Cintra,Dennys Esper; Ropelle,Eduardo Rochete; Rodrigues,Bruno; de Lira,Fabio Santos; de Souza,Claudio Teodoro

doi:10.3892/br.2015.514

November-2015 Volume 3 Issue 6

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November-2015 Volume 3 Issue 6

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Article

Topiramate effects lipolysis in 3T3-L1 adipocytes

Authors:
- Gabriela Poltronieri Campagnaro Martins
- Camila Oliveira Souza
- Scherolin de Oliveira Marques
- Thais Fernandes Luciano
- Bruno Luiz da Silva Pieri
- José César Rosa
- Adelino Sanchez Ramos da Silva
- José Rodrigo Pauli
- Dennys Esper Cintra
- Eduardo Rochete Ropelle
- Bruno Rodrigues
- Fabio Santos de Lira
- Claudio Teodoro de Souza
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Affiliations: Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806‑000, Brazil, Immunometabolism Research Group, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP 05508‑900, Brazil, School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP 14040‑907, Brazil, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP 13484‑350, Brazil, Laboratory of Human Movement, São Judas Tadeu University, São Paulo, SP 03166‑000, Brazil, Immunometabolism Research Group, Department of Physical Education, University Estadual Paulista (UNESP), Presidente Prudente, SP 19060‑900, Brazil
Pages: 827-830
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Published online on: August 28, 2015

https://doi.org/10.3892/br.2015.514
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Abstract

Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3‑L1. The 3T3‑L1 cells were incubated in 50 µM TPM for 30 min. The β‑adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non‑esterified fatty acid, glycerol and incorporation of 14C‑palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone‑sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI‑58) were assessed. The levels of glycerol and non‑esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI‑58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3‑L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Topiramate effects lipolysis in 3T3-L1 adipocytes

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Abstract

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