Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats

  • Authors:
    • Dan Yao
    • Weiran Zhang
    • Xue He
    • Jinhu Wang
    • Kewen Jiang
    • Zhengyan Zhao
  • View Affiliations

  • Published online on: February 23, 2016     https://doi.org/10.3892/br.2016.610
  • Pages: 437-443
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Abstract

The present study was designed to set up a reliable model of severe hypoxia‑ischemia brain damage (HIBD) in neonatal rats and several methods were used to identify whether the model was successful. A total of 40 healthy 7‑day‑old Sprague‑Dawley rats were randomly divided into 2 groups: The sham‑surgery group (n=18) and the HIBD model group (n=22). The HIBD model was produced according to the traditional Rice method. The rats were anesthetized with ethyl ether. The left common carotid artery (CCA) was exposed, ligated and cut. Following this, the rats were exposed to hypoxia in a normobaric chamber filled with 8% oxygen and 92% nitrogen for 2 h. In the sham‑surgery group, the left CCA was exposed but was not ligated, cut or exposed to hypoxia. The neurobehavioral changes of the rats were observed in the 24 h after HIBD. The brains were collected after 72 h to observe the pathological morphological changes of the brain tissue. The behavioral ability and neurobehavioral changes were studied in each group. The water maze test was used for evaluating the learning‑memory ability when the rats were 28 days old. Compared with the sham‑surgery group, all the HIBD model rats had a lag of motor development. The rats had evident changes in anatomy and Nissl staining, and cognitive impairment was shown through the result of the water maze. Therefore, the model of HIBD in neonatal rats is feasible and provides a reliable model for subsequent studies.
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April-2016
Volume 4 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Yao D, Zhang W, He X, Wang J, Jiang K and Zhao Z: Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats. Biomed Rep 4: 437-443, 2016
APA
Yao, D., Zhang, W., He, X., Wang, J., Jiang, K., & Zhao, Z. (2016). Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats. Biomedical Reports, 4, 437-443. https://doi.org/10.3892/br.2016.610
MLA
Yao, D., Zhang, W., He, X., Wang, J., Jiang, K., Zhao, Z."Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats". Biomedical Reports 4.4 (2016): 437-443.
Chicago
Yao, D., Zhang, W., He, X., Wang, J., Jiang, K., Zhao, Z."Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats". Biomedical Reports 4, no. 4 (2016): 437-443. https://doi.org/10.3892/br.2016.610