Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats

Yao,Dan; Zhang,Weiran; He,Xue; Wang,Jinhu; Jiang,Kewen; Zhao,Zhengyan

doi:10.3892/br.2016.610

April-2016 Volume 4 Issue 4

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April-2016 Volume 4 Issue 4

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Article

Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats

Authors:
- Dan Yao
- Weiran Zhang
- Xue He
- Jinhu Wang
- Kewen Jiang
- Zhengyan Zhao
View Affiliations / Copyright

Affiliations: Department of Pediatric Health Care, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Pediatric Surgery, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Neurology, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China
Pages: 437-443
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Published online on: February 23, 2016

https://doi.org/10.3892/br.2016.610
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Abstract

The present study was designed to set up a reliable model of severe hypoxia‑ischemia brain damage (HIBD) in neonatal rats and several methods were used to identify whether the model was successful. A total of 40 healthy 7‑day‑old Sprague‑Dawley rats were randomly divided into 2 groups: The sham‑surgery group (n=18) and the HIBD model group (n=22). The HIBD model was produced according to the traditional Rice method. The rats were anesthetized with ethyl ether. The left common carotid artery (CCA) was exposed, ligated and cut. Following this, the rats were exposed to hypoxia in a normobaric chamber filled with 8% oxygen and 92% nitrogen for 2 h. In the sham‑surgery group, the left CCA was exposed but was not ligated, cut or exposed to hypoxia. The neurobehavioral changes of the rats were observed in the 24 h after HIBD. The brains were collected after 72 h to observe the pathological morphological changes of the brain tissue. The behavioral ability and neurobehavioral changes were studied in each group. The water maze test was used for evaluating the learning‑memory ability when the rats were 28 days old. Compared with the sham‑surgery group, all the HIBD model rats had a lag of motor development. The rats had evident changes in anatomy and Nissl staining, and cognitive impairment was shown through the result of the water maze. Therefore, the model of HIBD in neonatal rats is feasible and provides a reliable model for subsequent studies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Establishment and identification of a hypoxia‑ischemia brain damage model in neonatal rats

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