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Article

Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism

  • Authors:
    • Majid Zarrin
    • Farzaneh Ganj
    • Sama Faramarzi
  • View Affiliations / Copyright

    Affiliations: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran, Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357-15794, Iran
  • Pages: 471-474
    |
    Published online on: February 25, 2016
       https://doi.org/10.3892/br.2016.615
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Abstract

The Fusarium species are a widely spread phytopathogen identified in an extensive variety of hosts. The Fusarium genus is one of the most heterogeneous fungi and is difficult to classify. Polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP) analysis is a useful method in detection of DNA polymorphism in objective sequences. The aim of the present study was to identify the phylogenetic associations and usefulness of the internal transcribed spacer (ITS) region as a genetic marker within the most clinically important strain of the Fusarium species. A total of 50 strains of Fusarium spp. were used in the study, including environmental, clinical and reference isolates. The primers ITS1 and ITS4 were used in the study. Two restriction enzymes, HaeIII and SmaI, were assessed for the digestion of PCR products. A PCR product of ~550‑base pairs was generated for each Fusarium species. The digested products with HaeIII and SmaI demonstrated that the bands generated for the medically significant Fusarium species, including F. solani, F. oxysporum, F. verticillidea, F. proliferatum and F. fujikuri, have different restriction enzyme patterns. In conclusion, it appears that the PCR‑RFLP method used in the present study produces a sufficient restriction profile for differentiation of the most medically significant Fusarium species.
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Copy and paste a formatted citation
Spandidos Publications style
Zarrin M, Ganj F and Faramarzi S: Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism. Biomed Rep 4: 471-474, 2016.
APA
Zarrin, M., Ganj, F., & Faramarzi, S. (2016). Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism. Biomedical Reports, 4, 471-474. https://doi.org/10.3892/br.2016.615
MLA
Zarrin, M., Ganj, F., Faramarzi, S."Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism". Biomedical Reports 4.4 (2016): 471-474.
Chicago
Zarrin, M., Ganj, F., Faramarzi, S."Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism". Biomedical Reports 4, no. 4 (2016): 471-474. https://doi.org/10.3892/br.2016.615
Copy and paste a formatted citation
x
Spandidos Publications style
Zarrin M, Ganj F and Faramarzi S: Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism. Biomed Rep 4: 471-474, 2016.
APA
Zarrin, M., Ganj, F., & Faramarzi, S. (2016). Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism. Biomedical Reports, 4, 471-474. https://doi.org/10.3892/br.2016.615
MLA
Zarrin, M., Ganj, F., Faramarzi, S."Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism". Biomedical Reports 4.4 (2016): 471-474.
Chicago
Zarrin, M., Ganj, F., Faramarzi, S."Analysis of the rDNA internal transcribed spacer region of the Fusarium species by polymerase chain reaction-restriction fragment length polymorphism". Biomedical Reports 4, no. 4 (2016): 471-474. https://doi.org/10.3892/br.2016.615
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