Interleukin-1β induces the upregulation of caveolin-1 expression in a rat brain tumor model

Qin,Li‑Juan; Jia,Yong‑Sen; Zhang,Yi‑Bing; Wang,Yin‑Huan

doi:10.3892/br.2016.618

Interleukin-1β induces the upregulation of caveolin-1 expression in a rat brain tumor model

Authors:
- Li‑Juan Qin
- Yong‑Sen Jia
- Yi‑Bing Zhang
- Yin‑Huan Wang
View Affiliations

Affiliations: Department of Physiology, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, Department of Chinese Medicine, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China
Published online on: February 26, 2016 https://doi.org/10.3892/br.2016.618
Pages: 433-436

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of the present study was to investigate the expression of caveolin-1 in rat brain glioma tissue, and to determine whether interleukin-1β (IL-1β) has a role in this process. Using glioma cells, a tumor‑burdened rat model was established, and the expression of caveolin-1 protein in the tumor sites was significantly increased following intracarotid infusion of IL-1β (3.7 ng/kg/min), as indicated by western blot analysis. The maximum value of the caveolin-1 expression was observed in tumor‑burdened rats after 60 min of IL‑1β perfusion, and which was significantly enhanced by vascular endothelial growth factor (VEGF). In addition, VEGF also significantly increased IL-1β-induced blood tumor barrier (BTB) permeability. The results suggest that the IL-1β-induced BTB permeability increase may be associated with the expression of caveolin-1 protein, and VEGF may be involved in this process.

View Figures

View References

1	Drappatz J, Schiff D, Kesari S, Norden AD and Wen PY: Medical management of brain tumor patients. Neurol Clin. 25:1035–1071, ix. 2007. View Article : Google Scholar : PubMed/NCBI
2	Laquintana V, Trapani A, Denora N, Wang F, Gallo JM and Trapani G: New strategies to deliver anticancer drugs to brain tumors. Expert Opin Drug Deliv. 6:1017–1032. 2009. View Article : Google Scholar : PubMed/NCBI
3	Sadowska GB, Chen X, Zhang J, Lim YP, Cummings EE, Makeyev O, Besio WG, Gaitanis J, Padbury JF, Banks WA and Stonestreet BS: Interleukin-1β transfer across the blood-brain barrier in the ovine fetus. J Cereb Blood Flow Metab. 35:1388–1395. 2015. View Article : Google Scholar : PubMed/NCBI
4	Komarova Y and Malik AB: Regulation of endothelial permeability via paracellular and transcellular transport pathways. Annu Rev Physiol. 72:463–493. 2010. View Article : Google Scholar : PubMed/NCBI
5	Salama NN, Eddington ND and Fasano A: Tight junction modulation and its relationship to drug delivery. Adv Drug Deliv Rev. 58:15–28. 2006. View Article : Google Scholar : PubMed/NCBI
6	Dohgu S, Fleegal-DeMotta MA and Banks WA: Lipopolysaccharide-enhanced transcellular transport of HIV-1 across the blood-brain barrier is mediated by luminal microvessel IL-6 and GM-CSF. J Neuroinflammation. 8:1672011. View Article : Google Scholar : PubMed/NCBI
7	Quest AF, Gutierrez-Pajares JL and Torres VA: Caveolin-1: An ambiguous partner in cell signalling and cancer. J Cell Mol Med. 12:1130–1150. 2008. View Article : Google Scholar : PubMed/NCBI
8	Sun SW, Zu XY, Tuo QH, Chen LX, Lei XY, Li K, Tang CK and Liao DF: Caveolae and caveolin-1 mediate endocytosis and transcytosis of oxidized low density lipoprotein in endothelial cells. Acta Pharmacol Sin. 31:1336–1342. 2010. View Article : Google Scholar : PubMed/NCBI
9	Nag S, Venugopalan R and Stewart DJ: Increased caveolin-1 expression precedes decreased expression of occludin and claudin-5 during blood-brain barrier breakdown. Acta Neuropathol. 114:459–469. 2007. View Article : Google Scholar : PubMed/NCBI
10	Ben Menachem, Zidon O, Ben Menahem Y, Ben Hur T and Yirmiya R: Intra- hippocampal transplantation of neural precursor cells with transgenic over- expression of IL-1 receptor antagonist rescues memory and neurogenesis impairments in an Alzheimer's disease model. Neuropsychopharmacology. 13:27362013.
11	Hayashi Y, Yoshida Y and Hamada J: Blood-tumor barrier in malignant brain tumor. No Shinkei Geka. 34:983–999. 2006.(In Japanese). PubMed/NCBI
12	Qin LJ, Xue YX, Gu YT, Zhang ZY, Zhang T and Sun N: Effect and mechanisms of interleukin-1β in process of opening the blood-brain barrier by bradykinin. Chinese Pharmacological Bulletin. 1:58–61. 2012.
13	Allan SM and Rothwell NJ: Cytokines and acute neurodegeneration. Nat Rev Neurosci. 2:734–744. 2001. View Article : Google Scholar : PubMed/NCBI
14	Davies DC: Blood-brain barrier breakdown in septic encephalopathy and brain tumours. J Anat. 200:639–646. 2002. View Article : Google Scholar : PubMed/NCBI
15	Faehling M, Kroll J, Föhr KJ, Fellbrich G, Mayr U, Trischler G and Waltenberger J: Essential role of calcium in vascular endothelial growth factor A-induced signaling: Mechanism of the antiangiogenic effect of carboxyamidotriazole. FASEB J. 16:1805–1807. 2002.PubMed/NCBI
16	Argaw AT, Asp L, Zhang J, Navrazhina K, Pham T, Mariani JN, Mahase S, Dutta DJ, Seto J, Kramer EG, et al: Astrocyte-derived VEGF-A drives blood-brain barrier disruption in CNS inflammatory disease. J Clin Invest. 122:2454–2468. 2012. View Article : Google Scholar : PubMed/NCBI
17	Wei M, Li H, Huang H, Xu D, Zhi D, Liu D and Zhang Y: Increased expression of EMMPRIN and VEGF in the rat brain after gamma irradiation. J Korean Med Sci. 27:291–299. 2012. View Article : Google Scholar : PubMed/NCBI
18	Kim J and Jung Y: Different expressions of AQP1, AQP4, eNOS, and VEGF proteins in ischemic versus non-ischemic cerebropathy in rats: Potential roles of AQP1 and eNOS in hydrocephalic and vasogenic edema formation. Anat Cell Biol. 44:295–303. 2011. View Article : Google Scholar : PubMed/NCBI
19	Lajoie P and Nabi IR: Regulation of raft-dependent endocytosis. J Cell Mol Med. 11:644–653. 2007. View Article : Google Scholar : PubMed/NCBI
20	Drab M, Verkade P, Elger M, Kasper M, Lohn M, Lauterbach B, Menne J, Lindschau C, Mende F, Luft FC, et al: Loss of caveolae, vascular dysfunction, and pulmonary defects in caveolin-1 gene-disrupted mice. Science. 293:2449–2452. 2001. View Article : Google Scholar : PubMed/NCBI
21	Zhong Y, Smart EJ, Weksler B, Couraud PO, Hennig B and Toborek M: Caveolin-1 regulates human immunodeficiency virus-1 Tat-induced alterations of tight junction protein expression via modulation of the Ras signaling. J Neurosci. 28:7788–7796. 2008. View Article : Google Scholar : PubMed/NCBI