Effects of bavachin and its regulation of melanin synthesis in A375 cells

Wang,Jing‑Hua; Pei,Yuan‑Yuan; Xu,Hong‑Dan; Li,Li‑Jing; Wang,Ye‑Qiu; Liu,Guo‑Liang; Qu,Yan; Zhang,Ning

doi:10.3892/br.2016.688

July-2016 Volume 5 Issue 1

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July-2016 Volume 5 Issue 1

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Article

Effects of bavachin and its regulation of melanin synthesis in A375 cells

Authors:
- Jing‑Hua Wang
- Yuan‑Yuan Pei
- Hong‑Dan Xu
- Li‑Jing Li
- Ye‑Qiu Wang
- Guo‑Liang Liu
- Yan Qu
- Ning Zhang
View Affiliations / Copyright

Affiliations: College of Pharmacy, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China, College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China, College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China
Pages: 87-92
|
Published online on: May 20, 2016

https://doi.org/10.3892/br.2016.688
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Abstract

The aim of the present study was to investigate the effect of bavachin treatment on A375 cells and the regulation of melanin synthesis. The cultured A375 cells in vitro were treated with bavachin; and the effect of bavachin on cell activity, tyrosinase (TYR) activity and melanin synthesis were respectively tested by the MTT assay, L‑dopa oxidation assay and the NaOH lysis assay. The expression levels of TYR and c‑Jun N‑terminal kinases (JNK) proteins were tested by western blot analysis. The expression levels of TYR, tyrosinase‑related protein‑1 (TRP‑1), TRP‑2, extracellular signal-regulated kinase 1 (ERK1), ERK2 and JNK2 mRNA were tested by the reverse transcription‑polymerase chain reaction assay. Simultaneously, the effect of estrogen receptor inhibitor (ICI182780) and ERK pathway inhibitor (U0126) was also tested on A375 cells following bavachin. The safe dose of bavachin significantly inhibited melanin synthesis and TYR activity. Bavachin (10 µmol/l) inhibited the expression of TYR and JNK proteins, and the expression of TYR, TRP‑1, TRP‑2, ERK1, ERK2 and JNK2 mRNA in A375 cells. ICI182780 and U0126 could significantly reverse the bavachin treatment on the protein expression levels and the mRNA expression of TYR, TRP‑1, TRP‑2, ERK1, ERK2 and JNK2. In conclusion, bavachin inhibited the synthesis of melanin on A375 cells by inhibiting the protein and mRNA expression of TYR, TRP-1, TRP-2, ERK1, ERK2 and JNK2.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of bavachin and its regulation of melanin synthesis in A375 cells

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