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Article

Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose

  • Authors:
    • Hongtao Hu
    • Shen Xu
    • Shuang Hu
    • Yue Gao
    • Hua Shui
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 699-704
    |
    Published online on: November 1, 2016
       https://doi.org/10.3892/br.2016.800
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Abstract

Deficiency in vitamin D and its active metabolite is a characteristic of chronic kidney diseases (CKDs). Previous studies have reported that 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], the active form of vitamin D, can attenuate renal interstitial fibrosis. The present study aimed to explore the effect of 1,25(OH)2D3 on the transdifferentiation of NRK‑52E rat renal tubular epithelial cells (RTECs) induced by high glucose, as well as the expression of vitamin D receptor (VDR) and production of angiotensin (Ang) Ⅱ. Western blot and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analyses were performed to detect the protein and mRNA expression of α‑smooth muscle actin (α‑SMA), E‑cadherin and VDR. Furthermore, the production of Ang Ⅱ was analyzed by enzyme‑linked immunosorbent assay (ELISA). Treatment with high glucose decreased E‑cadherin and VDR, while increasing α‑SMA and Ang Ⅱ, and of note, these changes were attenuated by 1,25(OH)2D3 in a dose‑dependent manner. In conclusion, the present study revealed that 1,25(OH)2D3 inhibits high glucose‑induced transdifferentiation of rat RTECs in a dose‑dependent manner, which may be associated with the downregulation of Ang Ⅱ and upregulation of VDR.
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Copy and paste a formatted citation
Spandidos Publications style
Hu H, Xu S, Hu S, Gao Y and Shui H: Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose. Biomed Rep 5: 699-704, 2016.
APA
Hu, H., Xu, S., Hu, S., Gao, Y., & Shui, H. (2016). Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose. Biomedical Reports, 5, 699-704. https://doi.org/10.3892/br.2016.800
MLA
Hu, H., Xu, S., Hu, S., Gao, Y., Shui, H."Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose". Biomedical Reports 5.6 (2016): 699-704.
Chicago
Hu, H., Xu, S., Hu, S., Gao, Y., Shui, H."Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose". Biomedical Reports 5, no. 6 (2016): 699-704. https://doi.org/10.3892/br.2016.800
Copy and paste a formatted citation
x
Spandidos Publications style
Hu H, Xu S, Hu S, Gao Y and Shui H: Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose. Biomed Rep 5: 699-704, 2016.
APA
Hu, H., Xu, S., Hu, S., Gao, Y., & Shui, H. (2016). Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose. Biomedical Reports, 5, 699-704. https://doi.org/10.3892/br.2016.800
MLA
Hu, H., Xu, S., Hu, S., Gao, Y., Shui, H."Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose". Biomedical Reports 5.6 (2016): 699-704.
Chicago
Hu, H., Xu, S., Hu, S., Gao, Y., Shui, H."Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose". Biomedical Reports 5, no. 6 (2016): 699-704. https://doi.org/10.3892/br.2016.800
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