Long non-coding RNA CCAT1 is overexpressed in oral squamous cell carcinomas and predicts poor prognosis

Arunkumar,Ganesan; Murugan,Avaniyapuram  Kannan; Prasanna Srinivasa Rao,Harikrishnan; Subbiah,Shanmugam; Rajaraman,Ramamurthy; Munirajan,Arasambattu  Kannan

doi:10.3892/br.2017.876

Long non-coding RNA CCAT1 is overexpressed in oral squamous cell carcinomas and predicts poor prognosis

Authors:
- Ganesan Arunkumar
- Avaniyapuram Kannan Murugan
- Harikrishnan Prasanna Srinivasa Rao
- Shanmugam Subbiah
- Ramamurthy Rajaraman
- Arasambattu Kannan Munirajan
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Affiliations: Department of Genetics, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India, Department of Molecular Oncology, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia, Center for Oncology, Royapettah Government Hospital and Kilpauk Medical College, Royapettah, Chennai 600 014, India
Published online on: March 17, 2017 https://doi.org/10.3892/br.2017.876
Pages: 455-462

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Abstract

Oral squamous cell carcinoma (SCC) is the most common malignant tumor in India with 5-year survival rates totaling <50%. Recently, dysregulation of non-coding RNA was reported as a potential hallmark of carcinogenesis. Colon Cancer Associated Transcript 1 (CCAT1), an lncRNA located in chromosome 8q24, close to the c‑Myc gene, has been reported to be overexpressed in many human cancers. In the present study, the authors analyzed the expression of CCAT1, c‑Myc and the miRNAs miR155-5p, let7b‑5p, miR490‑3p and miR218‑5p sponged by CCAT1 in 60 oral tumor and 8 normal tissue samples by reverse transcription‑quantitative polymerase chain reaction. CCAT1 was significantly overexpressed in 27% (16/60) of oral tumors. Interestingly, a high level of c‑Myc expression was observed in all CCAT1 overexpressing cases (P=0.0473). Furthermore, CCAT1 overexpression significantly downregulated miR155‑5p (P=0.03) and let7b‑5p (P<0.0001). Oral cancer cases expressing high level of CCAT1 (P=0.01) presented poor therapeutic outcome. To the best of the authors' knowledge, this is the first study to report the overexpression of the CCAT1 in oral SCCs, and the results suggested that CCAT1 overexpression may sponge miR155‑5p and let7b-5p, and may account for poor treatment response.

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