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Article

Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite

Corrigendum in: /10.3892/br.2019.1253
  • Authors:
    • Ayman G. Mustafa
    • Mahmoud A. Alfaqih
    • Othman Al‑Shboul
    • Ahmed Al‑Dwairi
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan, Department of Physiology and Biochemistry, School of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan
  • Pages: 421-426
    |
    Published online on: September 7, 2018
       https://doi.org/10.3892/br.2018.1144
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Abstract

Peroxynitrite can be produced in the vasculature from a superoxide anion reaction with nitric oxide. A surplus of peroxynitrite in the intravascular compartment is a common feature of several chronic diseases. The development of pharmacological modalities that interfere with the formation of peroxynitrite or inhibit its oxidative damage may be of utility for the prevention and/or treatment of several pathologies. Our previous investigations showed that catalytically inactivating peroxynitrite‑derived free radicals with tempol or scavenging reactive aldehyde species with phenelzine protects the blood plasma and platelets from the oxidative damage of peroxynitrite. However, the degree of inhibition of the cytotoxic effects of peroxynitrite using tempol or phenelzine was modest. In the present study, the aim was to examine if scavenging lipid peroxyl radicals with U‑83836E can achieve superior protection from peroxynitrite. This was assessed by treating blood plasma or platelets with 100 µM peroxynitrite alone or in combination with U‑83836E, and then measuring the levels of thiobarbituric acid reactive substances (TBARS) and protein carbonyl formation as indices of lipid peroxidation and protein oxidation, respectively. It was observed that scavenging lipid peroxyl radicals with 75‑100 µM U‑83836E increasingly reversed protein carbonylation induced by peroxynitrite in blood plasma and platelets, in addition to TBARS formation in blood plasma. These findings are further discussed in the context of the mechanisms by which U‑83836E may protect against the cell‑damaging effects of peroxynitrite.
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Copy and paste a formatted citation
Spandidos Publications style
Mustafa AG, Alfaqih MA, Al‑Shboul O and Al‑Dwairi A: Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253. Biomed Rep 9: 421-426, 2018.
APA
Mustafa, A.G., Alfaqih, M.A., Al‑Shboul, O., & Al‑Dwairi, A. (2018). Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253. Biomedical Reports, 9, 421-426. https://doi.org/10.3892/br.2018.1144
MLA
Mustafa, A. G., Alfaqih, M. A., Al‑Shboul, O., Al‑Dwairi, A."Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253". Biomedical Reports 9.5 (2018): 421-426.
Chicago
Mustafa, A. G., Alfaqih, M. A., Al‑Shboul, O., Al‑Dwairi, A."Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253". Biomedical Reports 9, no. 5 (2018): 421-426. https://doi.org/10.3892/br.2018.1144
Copy and paste a formatted citation
x
Spandidos Publications style
Mustafa AG, Alfaqih MA, Al‑Shboul O and Al‑Dwairi A: Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253. Biomed Rep 9: 421-426, 2018.
APA
Mustafa, A.G., Alfaqih, M.A., Al‑Shboul, O., & Al‑Dwairi, A. (2018). Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253. Biomedical Reports, 9, 421-426. https://doi.org/10.3892/br.2018.1144
MLA
Mustafa, A. G., Alfaqih, M. A., Al‑Shboul, O., Al‑Dwairi, A."Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253". Biomedical Reports 9.5 (2018): 421-426.
Chicago
Mustafa, A. G., Alfaqih, M. A., Al‑Shboul, O., Al‑Dwairi, A."Scavenging of lipid peroxyl radicals protects plasma lipids and proteins from peroxynitrite Corrigendum in /10.3892/br.2019.1253". Biomedical Reports 9, no. 5 (2018): 421-426. https://doi.org/10.3892/br.2018.1144
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