Upregulation of aryl hydrocarbon receptor nuclear translocator 2 in the hippocampi of post‑stroke depression rats

Pei,Chen‑Di; Zhang,Lu‑Lu; Fei,Peng‑Ge; Gu,Shi‑Na; Li,Wen‑Qiang; Mu,Jun‑Lin; Wang,Xu‑Sheng; Wang,Wen‑Sheng; Zhang,Xiang‑Yang; Zhang,Zhao‑Hui; Song,Jing‑Gui

doi:10.3892/br.2019.1225

Upregulation of aryl hydrocarbon receptor nuclear translocator 2 in the hippocampi of post‑stroke depression rats

Authors:
- Chen‑Di Pei
- Lu‑Lu Zhang
- Peng‑Ge Fei
- Shi‑Na Gu
- Wen‑Qiang Li
- Jun‑Lin Mu
- Xu‑Sheng Wang
- Wen‑Sheng Wang
- Xiang‑Yang Zhang
- Zhao‑Hui Zhang
- Jing‑Gui Song
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Affiliations: Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of General Medicine, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Henan Key Laboratory of Biological Psychiatry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Psychiatry and Behavioral Sciences, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA, Department of Psychosomatic Medicine, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453002, P.R. China
Published online on: June 24, 2019 https://doi.org/10.3892/br.2019.1225
Pages: 51-58
Copyright: © Pei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aryl hydrocarbon receptor nuclear translocator protein 2 (ARNT2), a member of the basic helix‑loop‑helix superfamily of transcription factors, may serve a vital role in neuronal survival and cell proliferation via formation of heterodimers with hypoxia‑inducible factor‑1α. Previous studies indicated that ARNT2 levels were elevated in the brains of ischemic rats; however, the involvement of ARNT2 in post‑stroke depression (PSD) rats is not well understood. Therefore, the present study aimed to investigate the levels of ARNT2 in the hippocampi of PSD rats, and to clarify the potential association between ARNT2 and behavioral performance. A PSD rat model was established by middle cerebral artery occlusion (MCAO) followed by a 4‑week chronic unpredictable mild stress (CUMS) regimen. A sucrose preference test and open field test (OFT) were conducted, and body weight was measured. In addition, reverse transcription‑polymerase chain reaction and immunohistochemistry were performed to measure ARNT expression. Results indicated that MCAO+CUMS rats had lower weight gain, consumed less sucrose and moved less compared with controls. Furthermore, the mRNA and protein levels of ARNT in MCAO+CUMS rats were increased compared with in controls. The sucrose preference index and horizontal movement distance in the OFT were positively correlated with ARNT mRNA level. Thus, from these findings it was suggested that ARNT2 may be positively associated with improvement of cognitive impairment, and therefore may be a potential target in PSD treatment.

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