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mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy

  • Authors:
    • Keisuke Fujitaka
    • Taku Murakami
    • Masato Takeuchi
    • Tetsuhiro Kakimoto
    • Hideki Mochida
    • Kenji Arakawa
  • View Affiliations / Copyright

    Affiliations: Research Unit/Frontier, Sohyaku Innovative Research Division, Mitsubishi Tanabe Pharma Corporation, Fujisawa‑shi, Kanagawa 251‑8555, Japan, Hitachi Chemical Co. America, Ltd., R&D Center, Irvine, CA 92617, USA, Medical Affairs Department, Ikuyaku Integrated Value Development Division, Mitsubishi Tanabe Pharma Corporation, Osaka 541‑8505, Japan, Medical Intelligence Department, Ikuyaku Integrated Value Development Division, Mitsubishi Tanabe Pharma Corporation, Tokyo 103‑8405, Japan
    Copyright: © Fujitaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 11
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    Published online on: November 12, 2020
       https://doi.org/10.3892/br.2020.1387
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Abstract

Urinary nano‑extracellular vesicles (NVs), including exosomes and microvesicles, are considered potential biomarkers for kidney diseases using liquid biopsies. However, clinical application of urinary NVs has not yet been validated. In the present study, the levels of mRNAs in urinary NVs in animal models of kidney disease were assessed. Urine samples were collected from the animal models and urinary NVs were isolated by ultracentrifugation. Gene expression levels of kidney injury markers in urinary NVs and renal tissue were quantified by reverse transcription‑quantitative PCR. The mRNA levels of desmin, a podocyte injury marker, in urinary NVs was markedly increased in the puromycin aminonucleoside (PAN) nephritis model, in parallel with enhanced desmin expression in kidney tissues. The expression of regulator of calcineurin 1 and the podocin to nephrin ratio (PNR) were also increased in the PAN nephritis model. Treatment with prednisolone mitigated these changes in gene expression as well as proteinuria. PNR, which is considered a predictive marker of glomerular dysfunction, in urinary NVs was highly correlated with urinary protein excretion (P<0.01). Furthermore, PNR in urinary NVs of Zucker diabetic fatty rats, a diabetic kidney disease model, was correlated with urinary albumin excretion (P<0.01). These results suggest that changes in mRNA levels of urinary NVs reflect the disease status of kidney tissues and their functional alterations. Collectively, mRNA analysis of urinary NVs may be used as a liquid biopsy tool for improved classification and performance of risk prediction to determine the severity of kidney diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Fujitaka K, Murakami T, Takeuchi M, Kakimoto T, Mochida H and Arakawa K: mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomed Rep 14: 11, 2021.
APA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., & Arakawa, K. (2021). mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomedical Reports, 14, 11. https://doi.org/10.3892/br.2020.1387
MLA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14.1 (2021): 11.
Chicago
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14, no. 1 (2021): 11. https://doi.org/10.3892/br.2020.1387
Copy and paste a formatted citation
x
Spandidos Publications style
Fujitaka K, Murakami T, Takeuchi M, Kakimoto T, Mochida H and Arakawa K: mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomed Rep 14: 11, 2021.
APA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., & Arakawa, K. (2021). mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomedical Reports, 14, 11. https://doi.org/10.3892/br.2020.1387
MLA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14.1 (2021): 11.
Chicago
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14, no. 1 (2021): 11. https://doi.org/10.3892/br.2020.1387
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