Open Access

mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy

  • Authors:
    • Keisuke Fujitaka
    • Taku Murakami
    • Masato Takeuchi
    • Tetsuhiro Kakimoto
    • Hideki Mochida
    • Kenji Arakawa
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  • Published online on: November 12, 2020     https://doi.org/10.3892/br.2020.1387
  • Article Number: 11
  • Copyright: © Fujitaka et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Urinary nano‑extracellular vesicles (NVs), including exosomes and microvesicles, are considered potential biomarkers for kidney diseases using liquid biopsies. However, clinical application of urinary NVs has not yet been validated. In the present study, the levels of mRNAs in urinary NVs in animal models of kidney disease were assessed. Urine samples were collected from the animal models and urinary NVs were isolated by ultracentrifugation. Gene expression levels of kidney injury markers in urinary NVs and renal tissue were quantified by reverse transcription‑quantitative PCR. The mRNA levels of desmin, a podocyte injury marker, in urinary NVs was markedly increased in the puromycin aminonucleoside (PAN) nephritis model, in parallel with enhanced desmin expression in kidney tissues. The expression of regulator of calcineurin 1 and the podocin to nephrin ratio (PNR) were also increased in the PAN nephritis model. Treatment with prednisolone mitigated these changes in gene expression as well as proteinuria. PNR, which is considered a predictive marker of glomerular dysfunction, in urinary NVs was highly correlated with urinary protein excretion (P<0.01). Furthermore, PNR in urinary NVs of Zucker diabetic fatty rats, a diabetic kidney disease model, was correlated with urinary albumin excretion (P<0.01). These results suggest that changes in mRNA levels of urinary NVs reflect the disease status of kidney tissues and their functional alterations. Collectively, mRNA analysis of urinary NVs may be used as a liquid biopsy tool for improved classification and performance of risk prediction to determine the severity of kidney diseases.
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January-2021
Volume 14 Issue 1

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Spandidos Publications style
Fujitaka K, Murakami T, Takeuchi M, Kakimoto T, Mochida H and Arakawa K: mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomed Rep 14: 11, 2021
APA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., & Arakawa, K. (2021). mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy. Biomedical Reports, 14, 11. https://doi.org/10.3892/br.2020.1387
MLA
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14.1 (2021): 11.
Chicago
Fujitaka, K., Murakami, T., Takeuchi, M., Kakimoto, T., Mochida, H., Arakawa, K."mRNAs in urinary nano‑extracellular vesicles as potential biomarkers for non‑invasive kidney biopsy". Biomedical Reports 14, no. 1 (2021): 11. https://doi.org/10.3892/br.2020.1387