Screening of anti‑chronic nonbacterial prostatitis activity of different extractions of the aerial part of Glycyrrhiza uralensis, and network pharmacology research
- Haifan Liu
- Jie Cui
- Lin Zhang
- Guanhua Chang
- Wenquan Wang
Affiliations: Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100193, P.R. China, Beijing University of Chinese Medicine, Beijing 102488, P.R. China
- Published online on: September 29, 2021 https://doi.org/10.3892/br.2021.1475
Copyright: © Liu
et al. This is an open access article distributed under the
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Commons Attribution License.
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In the present study, anti‑chronic nonbacterial prostatitis (CNP) pharmacological experiments using water and ethanol extraction of the aerial parts of Glycyrrhiza uralensis were performed to select the best active parts by comparing their efficacy in a CNP model established by injecting carrageenin into the ventral lobe of rat prostate. The anti‑CNP activities and expression of serum inflammatory factors in rats were also analyzed. A Protein‑Protein Interaction network was constructed, and core targets were screened using topology and analyzed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Water and ethanol extraction exhibited good inhibitory effect on the pathological changes of the prostate tissue, the expression of inflammatory factors and fibrosis factors in CNP rats, whereas no differences were observed compared with the positive control drug. Water extraction was more effective and significantly reduced PGE2 expression (P<0.05). Network pharmacology assays showed 15 active components in the aerial part of Glycyrrhiza uralensis, and 9 key CNP therapeutic targets of the aerial parts of Glycyrrhiza uralensis were identified. The effect of water exraction on chronic prostatitis rats was significant. The aerial part of Glycyrrhiza uralensis downregulated the levels of inflammatory factors and inhibited proinflammatory gene transcription, reduced oxidative stress response, inhibited cell survival pathways, regulated sex hormone levels, prevented immunostimulation and attenuated inflammation. This study provides a theoretical reference for the development of anti‑CNP agents, and offers a novel methodology for identifying and clarifying the mechanisms underlying the efficacy of the anti‑CNP components in the aerial part of Glycyrrhiza uralensis.