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Association between promoter methylation and gene expression of CGB3 and NOP56 in HPV‑infected cervical cancer cells

  • Authors:
    • Palak Singh
    • Kanwalat Chalertpet
    • Juthamard Sukbhattee
    • Nannabhat Wongmanee
    • Pimwipa Suwannakart
    • Pattamawadee Yanatatsaneejit
  • View Affiliations / Copyright

    Affiliations: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, Human Genetics Research Group, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
    Copyright: © Singh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1
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    Published online on: November 3, 2021
       https://doi.org/10.3892/br.2021.1484
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Abstract

Overexpression of the E7 gene of human papillomavirus (HPV) type 16 is one of the primary causes of cervical cancer. The E7 protein can bind with DNA methyltransferase I and induce methylation of tumor suppressor genes, such as cyclin‑A1 (CCNA1), leading to suppression of their expression, and thus, cancer progression. In the present study, the confirmation of methylation‑related expression of chorionic gonadotropin subunit 3 (CGB3) and nucleolar protein 56 (NOP56) genes in 5‑Azacytidine (5'‑aza)‑treated HPV16‑positive SiHa and HPV16‑negative C33A cell lines was shown. Using methylation‑specific‑PCR and quantitative PCR, the results showed that CGB3 and NOP56 methylation significantly decreased as the 5'‑aza concentration was increased, and this was inversely associated with their expression. Moreover, overexpression of E7 contributed to the augmentation of CGB3 and NOP56 methylation levels in C33A cells, resulting in a decrease in their expression. This study extends on previous observations of E7 HPV16 oncogenic function in terms of methylation‑repressing expression in more genes, which may be wholly applied to gene therapy in cervical cancer prevention.
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Copy and paste a formatted citation
Spandidos Publications style
Singh P, Chalertpet K, Sukbhattee J, Wongmanee N, Suwannakart P and Yanatatsaneejit P: Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells. Biomed Rep 16: 1, 2022.
APA
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., & Yanatatsaneejit, P. (2022). Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells. Biomedical Reports, 16, 1. https://doi.org/10.3892/br.2021.1484
MLA
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., Yanatatsaneejit, P."Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells". Biomedical Reports 16.1 (2022): 1.
Chicago
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., Yanatatsaneejit, P."Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells". Biomedical Reports 16, no. 1 (2022): 1. https://doi.org/10.3892/br.2021.1484
Copy and paste a formatted citation
x
Spandidos Publications style
Singh P, Chalertpet K, Sukbhattee J, Wongmanee N, Suwannakart P and Yanatatsaneejit P: Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells. Biomed Rep 16: 1, 2022.
APA
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., & Yanatatsaneejit, P. (2022). Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells. Biomedical Reports, 16, 1. https://doi.org/10.3892/br.2021.1484
MLA
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., Yanatatsaneejit, P."Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells". Biomedical Reports 16.1 (2022): 1.
Chicago
Singh, P., Chalertpet, K., Sukbhattee, J., Wongmanee, N., Suwannakart, P., Yanatatsaneejit, P."Association between promoter methylation and gene expression of <em>CGB3</em> and <em>NOP56</em> in HPV‑infected cervical cancer cells". Biomedical Reports 16, no. 1 (2022): 1. https://doi.org/10.3892/br.2021.1484
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