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Article

Artemisia vulgaris inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells

  • Authors:
    • Hoang Thanh Chi
    • Bui Thi Kim Ly
  • View Affiliations / Copyright

    Affiliations: Department of Medicine and Pharmacy, Thu Dau Mot University, Thu Dau Mot, Binh Duong 820000, Vietnam
  • Article Number: 92
    |
    Published online on: October 5, 2022
       https://doi.org/10.3892/br.2022.1575
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Abstract

In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. The aim of the present study was to determine and clarify the mechanism of action of AVM. BCR/ABL activation is present in >90% of CML cases. As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). The results revealed that AVM inhibited the phosphorylation of BCR/ABL and their subsequent molecular signals including AKT and MAPK activation. AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. Moreover, the effects of various sub‑fractions of AVM were then investigated in order to determine the optimal solvent for the identification of anticancer bioactive compounds. The results demonstrated that the ethyl acetate and chloroform fractions were more effective than the n‑hexane and water fractions. It is thus concluded that AVM inhibits the activity of BCR/ABL and their subsequent molecular signals, including AKT and MAPK, resulting in cytotoxicity via apoptosis.
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1 

Chereda B and Melo JV: Natural course and biology of CML. Ann Hematol. 94 (Suppl 2):S107–S121. 2015.PubMed/NCBI View Article : Google Scholar

2 

Hochhaus A, Larson RA, Guilhot F, Radich JP, Branford S, Hughes TP, Baccarani M, Deininger MW, Cervantes F, Fujihara S, et al: Long-term outcomes of imatinib treatment for chronic myeloid leukemia. N Engl J Med. 376:917–927. 2017.PubMed/NCBI View Article : Google Scholar

3 

Waller CF: Imatinib mesylate. Recent Results Cancer Res. 212:1–27. 2018.PubMed/NCBI View Article : Google Scholar

4 

Chandran RK, Geetha N, Sakthivel KM, Aswathy CG, Gopinath P, Raj TVA, Priya G, Nair JKKM and Sreedharan H: Genomic amplification of BCR-ABL1 fusion gene and its impact on the disease progression mechanism in patients with chronic myelogenous leukemia. Gene. 686:85–91. 2019.PubMed/NCBI View Article : Google Scholar

5 

Yurttas NO and Eskazan AE: Novel therapeutic approaches in chronic myeloid leukemia. Leuk Res. 91(106337)2020.PubMed/NCBI View Article : Google Scholar

6 

Yan Z, Lai Z and Lin J: Anticancer properties of traditional Chinese medicine. Comb Chem High Throughput Screen. 20:423–429. 2017.PubMed/NCBI View Article : Google Scholar

7 

Moreira D, Teixeira SS, Monteiro MHD, De-Oliveira AC and Paumgartten FJR: Traditional use and safety of herbal medicines1. Rev Bras Farmacogn. 24:248–257. 2014.

8 

van Agtmael MA, Eggelte TA and van Boxtel CJ: Artemisinin drugs in the treatment of malaria: From medicinal herb to registered medication. Trends Pharmacol Sci. 20:199–205. 1999.PubMed/NCBI View Article : Google Scholar

9 

Abiri R, Silva ALM, de Mesquita LSS, de Mesquita JWC, Atabaki N, de Almeida EB Jr, Shaharuddin NA and Malik S: Towards a better understanding of Artemisia vulgaris: Botany, phytochemistry, pharmacological and biotechnological potential. Food Res Int. 109:403–415. 2018.PubMed/NCBI View Article : Google Scholar

10 

Ly BTK, Ly DM, Linh PH, Son HK, Ha NL and Chi HT: Screening of medicinal herbs for cytotoxic activity to leukemia cells. J BUON. 25:1989–1996. 2020.PubMed/NCBI

11 

Li S, Ilaria RL Jr, Million RP, Daley GQ and Van Etten RA: The P190, P210, and P230 forms of the BCR/ABL oncogene induce a similar chronic myeloid leukemia-like syndrome in mice but have different lymphoid leukemogenic activity. J Exp Med. 189:1399–1412. 1999.PubMed/NCBI View Article : Google Scholar

12 

Ly BTK and Chi HT: The potential effects of green tea (-)-epigallocatechin-3-gallate on overcoming imatinib-resistance in chronic myeloid leukemia bearing BCR-ABL. Ho Chi Minh City University of Education: J Sci. 14:134–142. 2017.

13 

Ly BT, Chi HT, Yamagishi M, Kano Y, Hara Y, Nakano K, Sato Y and Watanabe T: Inhibition of FLT3 expression by green tea catechins in FLT3 mutated-AML cells. PLoS One. 8(e66378)2013.PubMed/NCBI View Article : Google Scholar

14 

Mahavorasirikul W, Viyanant V, Chaijaroenkul W, Itharat A and Na-Bangchang K: Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. BMC Complement Altern Med. 10(55)2010.PubMed/NCBI View Article : Google Scholar

15 

Arana-Trejo RM, Sánchez ER, Ignacio-Ibarra G, de la Fuente EB, Garces O, Morales EG, Granados MC, Martínez RO, Rubio-Borja ME, Anaya LS, et al: BCR/ABL p210, p190 and p230 fusion genes in 250 Mexican patients with chronic myeloid leukaemia (CML). Clin Lab Haematol. 24:145–150. 2002.PubMed/NCBI View Article : Google Scholar

16 

Hehlmann R, Lauseker M, Saußele S, Pfirrmann M, Krause S, Kolb HJ, Neubauer A, Hossfeld DK, Nerl C, Gratwohl A, et al: Assessment of imatinib as first-line treatment of chronic myeloid leukemia: 10-year survival results of the randomized CML study IV and impact of non-CML determinants. Leukemia. 31:1476–5551. 2017.PubMed/NCBI View Article : Google Scholar

17 

Branford S, Melo JV and Hughes TP: Selecting optimal second-line tyrosine kinase inhibitor therapy for chronic myeloid leukemia patients after imatinib failure: Does the BCR-ABL mutation status really matter? Blood. 114:5426–5435. 2009.PubMed/NCBI View Article : Google Scholar

18 

Apperley JF: Part I: Mechanisms of resistance to imatinib in chronic myeloid leukaemia. Lancet Oncol. 8:1018–1029. 2007.PubMed/NCBI View Article : Google Scholar

19 

Bray F, Laversanne M, Weiderpass E and Soerjomataram I: The ever-increasing importance of cancer as a leading cause of premature death worldwide. Cancer. 127:3029–3030. 2021.PubMed/NCBI View Article : Google Scholar

20 

Mauro MJ and Druker BJ: STI571: Targeting BCR-ABL as therapy for CML. Oncologist. 6:233–238. 2001.PubMed/NCBI View Article : Google Scholar

21 

Herbst RS: Erlotinib (Tarceva): An update on the clinical trial program. Semin Oncol. 30:34–46. 2003.PubMed/NCBI

22 

Cragg GM and Pezzuto JM: Natural products as a vital source for the discovery of cancer chemotherapeutic and chemopreventive agents. Med Princ Pract. 25 (Suppl 2):S41–S59. 2016.PubMed/NCBI View Article : Google Scholar

23 

Abad MJ, Bedoya LM, Apaza L and Bermejo P: The artemisia L. Genus: A review of bioactive essential oils. Molecules. 17:2542–2566. 2012.PubMed/NCBI View Article : Google Scholar

24 

Sharmila K and Padma PR: Anticancer activity of Artemisia vulgaris on hepatocellular carcinoma (HepG2) cells. Int J Pharmacy Pharm Sci. 5:479–483. 2013.

25 

Saleh AM, Aljada A, Rizvi SA, Nasr A, Alaskar AS and Williams JD: In vitro cytotoxicity of Artemisia vulgaris L. essential oil is mediated by a mitochondria-dependent apoptosis in HL-60 leukemic cell line. BMC Complement Altern Med. 14(226)2014.PubMed/NCBI View Article : Google Scholar

26 

Erel SB, Şenol SK, Köse FA and Ballar P: In vitro cytotoxic properties of six Artemisia L. species. Turk J Pharm Sci. 8:247–252. 2011.

27 

Deguchi Y, Kimura S, Ashihara E, Niwa T, Hodohara K, Fujiyama Y and Maekawa T: Comparison of imatinib, dasatinib, nilotinib and INNO-406 in imatinib-resistant cell lines. Leuk Res. 32:980–983. 2008.PubMed/NCBI View Article : Google Scholar

28 

Liu C, Nie D, Li J, Du X, Lu Y, Li Y, Zhou J, Jin Y and Pan J: Antitumor effects of blocking protein neddylation in T315I-BCR-ABL leukemia cells and leukemia stem cells. Cancer Res. 78:1522–1536. 2018.PubMed/NCBI View Article : Google Scholar

29 

Burslem GM, Schultz AR, Bondeson DP, Eide CA, Stevens SL, Druker BJ and Crews CM: Targeting BCR-ABL1 in chronic myeloid leukemia by PROTAC-mediated targeted protein degradationdual inhibition and degradation of BCR-ABL1. Cancer Res. 79:4744–4753. 2019.PubMed/NCBI View Article : Google Scholar

30 

Huang H, Weng H, Dong B, Zhao P, Zhou H and Qu L: Oridonin triggers chaperon-mediated proteasomal degradation of BCR-ABL in leukemia. Sci Rep. 7:1–12. 2017.PubMed/NCBI View Article : Google Scholar

31 

Shibata N, Ohoka N, Tsuji G, Demizu Y, Miyawaza K, Ui-Tei K, Akiyama T and Naito M: Deubiquitylase USP25 prevents degradation of BCR-ABL protein and ensures proliferation of Ph-positive leukemia cells. Oncogene. 39:3867–3878. 2020.PubMed/NCBI View Article : Google Scholar

32 

Jiang S, Wang X, He Y, Huang H, Cao B, Zhang Z, Liu J, Wang Q, Huang Z and Mao X: Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis. Cell Death Dis. 12(456)2021.PubMed/NCBI View Article : Google Scholar

33 

Carrà G, Panuzzo C, Crivellaro S, Morena D, Taulli R, Guerrasio A, Saglio G and Morotti A: The targetable role of herpes virus-associated ubiquitin-specific protease (HAUSP) in p190 BCR-ABL leukemia. Oncol Lett. 12:3123–3126. 2016.PubMed/NCBI View Article : Google Scholar

34 

Efferth T, Dunstan H, Sauerbrey A, Miyachi H and Chitambar CR: The anti-malarial artesunate is also active against cancer. Int J Oncol. 18:767–773. 2001.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Chi HT and Ly BT: <em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells. Biomed Rep 17: 92, 2022.
APA
Chi, H.T., & Ly, B.T. (2022). <em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells. Biomedical Reports, 17, 92. https://doi.org/10.3892/br.2022.1575
MLA
Chi, H. T., Ly, B. T."<em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells". Biomedical Reports 17.6 (2022): 92.
Chicago
Chi, H. T., Ly, B. T."<em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells". Biomedical Reports 17, no. 6 (2022): 92. https://doi.org/10.3892/br.2022.1575
Copy and paste a formatted citation
x
Spandidos Publications style
Chi HT and Ly BT: <em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells. Biomed Rep 17: 92, 2022.
APA
Chi, H.T., & Ly, B.T. (2022). <em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells. Biomedical Reports, 17, 92. https://doi.org/10.3892/br.2022.1575
MLA
Chi, H. T., Ly, B. T."<em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells". Biomedical Reports 17.6 (2022): 92.
Chicago
Chi, H. T., Ly, B. T."<em>Artemisia vulgaris</em> inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells". Biomedical Reports 17, no. 6 (2022): 92. https://doi.org/10.3892/br.2022.1575
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