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Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling

  • Authors:
    • Walaa Bayoumie El Gazzar
    • Mona Maher Allam
    • Sherif Ahmed Shaltout
    • Lina Abdelhady Mohammed
    • Ashraf Mohamed Sadek
    • Hend Elsayed Nasr
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Physiology and Biochemistry, Faculty of Medicine, Hashemite University, Zarqa 13133, Jordan, Department of Physiology, Faculty of Medicine, Benha University, Benha 13518, Egypt, Department of Pharmacology, Public Health and Clinical Skills, Faculty of Medicine, Hashemite University, Zarqa 13133, Jordan, Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha 13518, Egypt
    Copyright: © El Gazzar et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
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    Published online on: November 16, 2022
       https://doi.org/10.3892/br.2022.1584
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Abstract

Acute kidney injury (AKI) is commonly a result of renal ischemia reperfusion injury (IRI), which produces clinical complications characterized by the rapid deterioration of renal function, leading to chronic kidney disease and increases the risk of morbidity and mortality. Currently, only supportive treatment is available. AKI, which is accompanied by immune activation and inflammation, is caused by proximal tubular injury. The present study investigated the role of tubular epithelial cells as drivers of inflammation in renal IRI and their potential function as antigen‑presenting cells, as well as the molecular mechanisms by which peroxisome proliferator‑activated receptor‑γ (PPARγ) agonists [such as pioglitazone (Pio)] exert reno‑protective action in renal IRI. A total of 50 Wistar male albino rats were divided into five groups: Sham + DMSO, Sham + Pio, IRI + DMSO, IRI + prophylactic preoperative (pre) Pio and IRI + postoperative Pio. The histopathological changes in renal tissue samples and the renal epithelial cell expression of CD86, miRNA‑124, STAT3, pro‑inflammatory cytokines, inducible nitric oxide synthase (iNOS) and Arginase‑II were analyzed by immunohistochemistry, reverse transcription‑quantitative PCR, western blotting and ELISA respectively. IRI was a potent inducer for CD86 immunoexpression. An ameliorative action of Pio was demonstrated via decreased CD86 immunoexpression, upregulation of miRNA‑124, decreased STAT3 expression and beneficial anti‑inflammatory effects. The tubular epithelium served a notable role in the inflammatory response in renal IRI. Pio exerted its anti‑inflammatory effects via PPARγ/miRNA‑124/STAT3 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
El Gazzar WB, Allam MM, Shaltout SA, Mohammed LA, Sadek AM and Nasr HE: Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling. Biomed Rep 18: 2, 2023.
APA
El Gazzar, W.B., Allam, M.M., Shaltout, S.A., Mohammed, L.A., Sadek, A.M., & Nasr, H.E. (2023). Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling. Biomedical Reports, 18, 2. https://doi.org/10.3892/br.2022.1584
MLA
El Gazzar, W. B., Allam, M. M., Shaltout, S. A., Mohammed, L. A., Sadek, A. M., Nasr, H. E."Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling". Biomedical Reports 18.1 (2023): 2.
Chicago
El Gazzar, W. B., Allam, M. M., Shaltout, S. A., Mohammed, L. A., Sadek, A. M., Nasr, H. E."Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling". Biomedical Reports 18, no. 1 (2023): 2. https://doi.org/10.3892/br.2022.1584
Copy and paste a formatted citation
x
Spandidos Publications style
El Gazzar WB, Allam MM, Shaltout SA, Mohammed LA, Sadek AM and Nasr HE: Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling. Biomed Rep 18: 2, 2023.
APA
El Gazzar, W.B., Allam, M.M., Shaltout, S.A., Mohammed, L.A., Sadek, A.M., & Nasr, H.E. (2023). Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling. Biomedical Reports, 18, 2. https://doi.org/10.3892/br.2022.1584
MLA
El Gazzar, W. B., Allam, M. M., Shaltout, S. A., Mohammed, L. A., Sadek, A. M., Nasr, H. E."Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling". Biomedical Reports 18.1 (2023): 2.
Chicago
El Gazzar, W. B., Allam, M. M., Shaltout, S. A., Mohammed, L. A., Sadek, A. M., Nasr, H. E."Pioglitazone modulates immune activation and ameliorates inflammation induced by injured renal tubular epithelial cells via PPARγ/miRNA‑124/STAT3 signaling". Biomedical Reports 18, no. 1 (2023): 2. https://doi.org/10.3892/br.2022.1584
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