Andrographis paniculata methanol extract suppresses the phosphorylation of ETV6‑NTRK3

  • Authors:
    • Hoang Thanh Chi
    • Vo Ngoc Tram
    • Nguyen Trung Quan
    • Bui Thi Kim Ly
  • View Affiliations

  • Published online on: June 8, 2023     https://doi.org/10.3892/br.2023.1630
  • Article Number: 47
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Abstract

ETS variant transcription factor 6 (ETV6)-neurotrophic receptor tyrosine kinase 3 (NTRK3) (EN) fusions are typically found in rare diseases, such as primary renal fibrosarcoma (only six cases have been reported), secretory carcinoma of the breast and salivary gland (1 case), and AML (4 cases). Few cases have been reported, and expression of the EN gene fusion requires additional clinical data and fundamental research to be supported. The aim of the present study was to determine the inhibitory effect of Andrographis paniculata methanol extract (MeAP) on EN‑related cell lines, IMS‑M2 and BaF3/EN, as well as evaluate the mechanism of action. Vero cells were used as control cells. Trypan blue staining and MTT were used to evaluate the inhibitory effect of MeAP on tested cells. Western blotting and immunoprecipitation were used to detect the activation of EN after MeAP treatment. The IC50 values of MeAP were found to be 12.38±0.57 µg/ml (IMS‑M2) and 13.06±0.49 µg/ml (BaF3/EN). MeAP was observed to inhibit cell proliferation in a time, dose, and cell density‑dependent manner. The IC50 value for MeAP in Vero cells was markedly higher, at 109.97±4.24 (µg/ml), indicating a much less sensitive effect. Furthermore, MeAP treatment inhibited EN phosphorylation and induced apoptosis in these cells. Collectively, the present study demonstrated that MeAP has an oncogenic effect on EN fusion‑positive cell lines, in particular.
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July-2023
Volume 19 Issue 1

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Spandidos Publications style
Chi HT, Tram VN, Quan NT and Ly BT: <em>Andrographis paniculata</em> methanol extract suppresses the phosphorylation of ETV6‑NTRK3. Biomed Rep 19: 47, 2023
APA
Chi, H.T., Tram, V.N., Quan, N.T., & Ly, B.T. (2023). <em>Andrographis paniculata</em> methanol extract suppresses the phosphorylation of ETV6‑NTRK3. Biomedical Reports, 19, 47. https://doi.org/10.3892/br.2023.1630
MLA
Chi, H. T., Tram, V. N., Quan, N. T., Ly, B. T."<em>Andrographis paniculata</em> methanol extract suppresses the phosphorylation of ETV6‑NTRK3". Biomedical Reports 19.1 (2023): 47.
Chicago
Chi, H. T., Tram, V. N., Quan, N. T., Ly, B. T."<em>Andrographis paniculata</em> methanol extract suppresses the phosphorylation of ETV6‑NTRK3". Biomedical Reports 19, no. 1 (2023): 47. https://doi.org/10.3892/br.2023.1630