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Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B

  • Authors:
    • Qingwu Liao
    • Fang Li
    • Mingming Xue
    • Wenan Chen
    • Zhengang Tao
    • Yuejiao Song
    • Ying Yuan
  • View Affiliations / Copyright

    Affiliations: Shanghai Key Laboratory of Perioperative Stress and Protection, Department of Anesthesia, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Geriatrics, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, Fujian 361015, P.R. China, Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Emergency, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Anesthesia, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen, Fujian 361015, P.R. China, Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 102
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    Published online on: October 26, 2023
       https://doi.org/10.3892/br.2023.1684
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Abstract

Sepsis‑induced acute lung injury (ALI) is related to the dysregulation of inflammatory responses. Polydatin supplement was reported to exhibit anti‑inflammatory effects in several diseases. The present study aimed to investigate the role of polydatin in sepsis‑induced ALI. A cecum ligation and puncture (CLP)‑induced mouse ALI model was established first and the pathological changes of lung tissues were assessed using hematoxylin and eosin staining. Meanwhile, to mimic sepsis‑induced ALI in vitro, pulmonary microvascular endothelial cells (PMVECs) were treated with lipopolysaccharide (LPS). Pro‑inflammatory cytokines levels were measured in lung tissues and PMVECs using ELISA. Reverse transcription‑quantitative PCR was used to measure the mRNA levels of Spi‑B in lung tissues and PMVECs. Moreover, the expression levels of Spi‑B, p‑PI3K, p‑Akt, and p‑NF‑κB in lung tissues and PMVECs were determined using western blotting. The data revealed that polydatin attenuated CLP‑induced lung injury and inhibited sepsis‑induced inflammatory responses in mice. Furthermore, polydatin significantly inhibited the expression of Spi‑B, p‑PI3K, p‑Akt, and p‑NF‑κB in lung tissues of mice subjected to CLP‑induced ALI, while this phenomenon was reversed through Spi‑B overexpression. Consistently, the anti‑inflammatory effect of polydatin was abolished by Spi‑B overexpression. Taken together, the current findings revealed that polydatin alleviated sepsis‑induced ALI via the downregulation of Spi‑B.
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Copy and paste a formatted citation
Spandidos Publications style
Liao Q, Li F, Xue M, Chen W, Tao Z, Song Y and Yuan Y: Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B. Biomed Rep 19: 102, 2023.
APA
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., & Yuan, Y. (2023). Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B. Biomedical Reports, 19, 102. https://doi.org/10.3892/br.2023.1684
MLA
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., Yuan, Y."Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B". Biomedical Reports 19.6 (2023): 102.
Chicago
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., Yuan, Y."Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B". Biomedical Reports 19, no. 6 (2023): 102. https://doi.org/10.3892/br.2023.1684
Copy and paste a formatted citation
x
Spandidos Publications style
Liao Q, Li F, Xue M, Chen W, Tao Z, Song Y and Yuan Y: Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B. Biomed Rep 19: 102, 2023.
APA
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., & Yuan, Y. (2023). Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B. Biomedical Reports, 19, 102. https://doi.org/10.3892/br.2023.1684
MLA
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., Yuan, Y."Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B". Biomedical Reports 19.6 (2023): 102.
Chicago
Liao, Q., Li, F., Xue, M., Chen, W., Tao, Z., Song, Y., Yuan, Y."Polydatin alleviates sepsis‑induced acute lung injury via downregulation of Spi‑B". Biomedical Reports 19, no. 6 (2023): 102. https://doi.org/10.3892/br.2023.1684
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