Open Access

Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe2ZnO4 nanoparticles against MCF‑7 cell line

  • Authors:
    • Asma' Al-Zabin
    • Tuqa Abu Thiab
    • Malek Zihlif
    • Afnan Al-Hunaiti
    • Hamzeh J Al-Ameer
    • Wajdy Al-Awaida
    • Amer Imraish
  • View Affiliations

  • Published online on: January 10, 2024     https://doi.org/10.3892/br.2024.1724
  • Article Number: 36
  • Copyright: © Al-Zabin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The use of plants for nanoparticle (NP) synthesis, grounded in green chemistry principles, is an environmentally friendly and economically viable approach. In the present study, the leaf extract of Elaeagnus angustifolia L. was used as a biosynthetic agent to generate bimetallic zinc oxide NPs. The present study investigated the effect of ZnO NPs on anti‑angiogenesis and cell migration. Various bimetallic NPs, including zinc‑iron oxide and nickel‑zinc oxide, underwent characterization through Fourier‑transform infrared spectroscopy and X‑ray Diffraction within the 25‑65˚ range. Confirmation of NP formation was determined by identifying the surface plasmon resonance peak. MTT assay was used to determine the cytotoxic properties of E. angustifolia L. extracts, ZnO NPs and associated metals in MCF‑7 breast cancer cells. The plant extract demonstrated antiproliferative effects at 200 µg/ml, whereas E. ang‑Fe2ZnO4 NPs showed varying cytotoxic effects based on concentration. The rat aortic ring and cell migration assays illuminated anti‑angiogenic attributes, with the E. ang‑Fe2ZnO4 NPs blocking blood vessel development entirely at 100 µg/ml, implying profound anti‑angiogenic efficacy. Therefore, E. ang‑Fe2ZnO4 NPs may serve a role in antiangiogenic therapy.
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March-2024
Volume 20 Issue 3

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Spandidos Publications style
Al-Zabin A, Abu Thiab T, Zihlif M, Al-Hunaiti A, Al-Ameer HJ, Al-Awaida W and Imraish A: Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomed Rep 20: 36, 2024
APA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H.J., Al-Awaida, W., & Imraish, A. (2024). Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomedical Reports, 20, 36. https://doi.org/10.3892/br.2024.1724
MLA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20.3 (2024): 36.
Chicago
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20, no. 3 (2024): 36. https://doi.org/10.3892/br.2024.1724