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Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe2ZnO4 nanoparticles against MCF‑7 cell line

  • Authors:
    • Asma' Al-Zabin
    • Tuqa Abu Thiab
    • Malek Zihlif
    • Afnan Al-Hunaiti
    • Hamzeh J Al-Ameer
    • Wajdy Al-Awaida
    • Amer Imraish
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, School of Science, Biology Building, The University of Jordan, Amman 11942, Jordan, Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman 11942, Jordan, Department of Chemistry, School of Science, The University of Jordan, Amman 11942, Jordan, Department of Biotechnology, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, Jordan, Department of Biology and Biotechnology, Faculty of Science, American University of Madaba, Madaba 17110, Jordan
    Copyright: © Al-Zabin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 36
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    Published online on: January 10, 2024
       https://doi.org/10.3892/br.2024.1724
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Abstract

The use of plants for nanoparticle (NP) synthesis, grounded in green chemistry principles, is an environmentally friendly and economically viable approach. In the present study, the leaf extract of Elaeagnus angustifolia L. was used as a biosynthetic agent to generate bimetallic zinc oxide NPs. The present study investigated the effect of ZnO NPs on anti‑angiogenesis and cell migration. Various bimetallic NPs, including zinc‑iron oxide and nickel‑zinc oxide, underwent characterization through Fourier‑transform infrared spectroscopy and X‑ray Diffraction within the 25‑65˚ range. Confirmation of NP formation was determined by identifying the surface plasmon resonance peak. MTT assay was used to determine the cytotoxic properties of E. angustifolia L. extracts, ZnO NPs and associated metals in MCF‑7 breast cancer cells. The plant extract demonstrated antiproliferative effects at 200 µg/ml, whereas E. ang‑Fe2ZnO4 NPs showed varying cytotoxic effects based on concentration. The rat aortic ring and cell migration assays illuminated anti‑angiogenic attributes, with the E. ang‑Fe2ZnO4 NPs blocking blood vessel development entirely at 100 µg/ml, implying profound anti‑angiogenic efficacy. Therefore, E. ang‑Fe2ZnO4 NPs may serve a role in antiangiogenic therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Al-Zabin A, Abu Thiab T, Zihlif M, Al-Hunaiti A, Al-Ameer HJ, Al-Awaida W and Imraish A: Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomed Rep 20: 36, 2024.
APA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H.J., Al-Awaida, W., & Imraish, A. (2024). Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomedical Reports, 20, 36. https://doi.org/10.3892/br.2024.1724
MLA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20.3 (2024): 36.
Chicago
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20, no. 3 (2024): 36. https://doi.org/10.3892/br.2024.1724
Copy and paste a formatted citation
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Spandidos Publications style
Al-Zabin A, Abu Thiab T, Zihlif M, Al-Hunaiti A, Al-Ameer HJ, Al-Awaida W and Imraish A: Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomed Rep 20: 36, 2024.
APA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H.J., Al-Awaida, W., & Imraish, A. (2024). Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line. Biomedical Reports, 20, 36. https://doi.org/10.3892/br.2024.1724
MLA
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20.3 (2024): 36.
Chicago
Al-Zabin, A., Abu Thiab, T., Zihlif, M., Al-Hunaiti, A., Al-Ameer, H. J., Al-Awaida, W., Imraish, A."Anti‑angiogenic and cytotoxic evaluation of green‑synthesized Fe<sub>2</sub>ZnO<sub>4</sub> nanoparticles against MCF‑7 cell line". Biomedical Reports 20, no. 3 (2024): 36. https://doi.org/10.3892/br.2024.1724
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