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Ca2+ signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder

  • Authors:
    • Magdalena P. Cortés
    • Catalina Alonso
    • Raúl Vinet
    • Karla Valdivia‑Cortés
    • Leonel Muñoz‑Sagredo
    • Tania F. Bahamondez‑Canas
    • Ana María Cárdenas
  • View Affiliations / Copyright

    Affiliations: School of Chemistry and Pharmacy, Faculty of Pharmacy, University of Valparaíso, Valparaíso 2360102, Chile, Gynecology and Obstetrics Service, Dr Gustavo Fricke Hospital, Viña del Mar‑Quillota Health Service, Viña del Mar 2570017, Chile, School of Medicine, Faculty of Medicine, University of Valparaíso, Viña del Mar 2540064, Chile, Interdisciplinary Neuroscience Center of Valparaíso, Faculty of Sciences, University of Valparaíso, Valparaíso 2360102, Chile
    Copyright: © Cortés et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 76
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    Published online on: March 14, 2024
       https://doi.org/10.3892/br.2024.1764
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Abstract

Fetal growth restriction associated with hypertensive disorders of pregnancy (FGR‑HDP) is a prevalent pathology with a higher risk of perinatal morbimortality. In this condition, placental insufficiency and endothelial dysfunction serve key roles. The present prospective cohort study monitored 11 patients with an FGR‑HDP and 15 with full‑term normotensive pregnancies and studied post‑natal intracellular calcium concentration ([Ca2+]i) signals in human umbilical vein endothelial cells (HUVECs). Small fetuses with placental insufficiency were identified using fetal biometry with Doppler velocimetry. Mean gestational age and birth weight were 31.8±4.1 weeks and 1,260±646 g for FGR‑HDP and 39.2±0.8 weeks and 3,320±336 g for normal births, respectively. Abnormal umbilical artery Doppler waveforms were found in 64% of neonates with FGR‑HDP. A significant percentage (86%) of FGR newborns were admitted to the neonatal intensive care unit at Gustavo Fricke hospital, Viña del Mar, Chile, with one case of death after birth. [Ca2+]i signals were measured by microfluorimetry in Fluo‑3‑loaded HUVECs from primary cultures. Altered [Ca2+]i signals were observed in HUVECs from FGR‑HDP, where the sustained phase of ATP‑induced [Ca2+]i responses was significantly reduced compared with the normotensive group. Also, the [Ca2+]i signals induced with 10 mM Ca2+ after depletion of internal Ca2+ stores were significantly higher. The present study provides a better comprehension of the role of altered cytosolic Ca2+ dynamics in endothelial dysfunction and an in vitro model to assess novel therapeutic approaches for decreasing or preventing complications in FGR‑HDP.
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Copy and paste a formatted citation
Spandidos Publications style
Cortés MP, Alonso C, Vinet R, Valdivia‑Cortés K, Muñoz‑Sagredo L, Bahamondez‑Canas TF and Cárdenas AM: Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder. Biomed Rep 20: 76, 2024.
APA
Cortés, M.P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T.F., & Cárdenas, A.M. (2024). Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder. Biomedical Reports, 20, 76. https://doi.org/10.3892/br.2024.1764
MLA
Cortés, M. P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T. F., Cárdenas, A. M."Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder". Biomedical Reports 20.5 (2024): 76.
Chicago
Cortés, M. P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T. F., Cárdenas, A. M."Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder". Biomedical Reports 20, no. 5 (2024): 76. https://doi.org/10.3892/br.2024.1764
Copy and paste a formatted citation
x
Spandidos Publications style
Cortés MP, Alonso C, Vinet R, Valdivia‑Cortés K, Muñoz‑Sagredo L, Bahamondez‑Canas TF and Cárdenas AM: Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder. Biomed Rep 20: 76, 2024.
APA
Cortés, M.P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T.F., & Cárdenas, A.M. (2024). Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder. Biomedical Reports, 20, 76. https://doi.org/10.3892/br.2024.1764
MLA
Cortés, M. P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T. F., Cárdenas, A. M."Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder". Biomedical Reports 20.5 (2024): 76.
Chicago
Cortés, M. P., Alonso, C., Vinet, R., Valdivia‑Cortés, K., Muñoz‑Sagredo, L., Bahamondez‑Canas, T. F., Cárdenas, A. M."Ca<sup>2+</sup> signals in human umbilical endothelial cells derived from pregnancy with fetal growth restriction associated with hypertensive disorder". Biomedical Reports 20, no. 5 (2024): 76. https://doi.org/10.3892/br.2024.1764
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