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Gene expression alterations in hypoxic A549 lung cancer cell line

  • Authors:
    • Rania Abualnadi
    • Nafez Abu Tarboush
    • Mohammad Shhab
    • Malek Zihlif
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, School of Medicine, The University of Jordan, Amman 11942, Jordan, Department of Physiology and Biochemistry, School of Medicine, The University of Jordan, Amman 11942, Jordan
    Copyright: © Abualnadi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 183
    |
    Published online on: October 4, 2024
       https://doi.org/10.3892/br.2024.1871
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Abstract

Human non‑small cell lung cancer (NSCLC)is a very common disease with limited treatment options. Hypoxia is a characteristic feature of solid tumors associated with the resistance of cancer cells to radiotherapy and chemotherapy. Therefore, the expression changes in cancer‑resistance genes may be biomarkers of hypoxia with value in targeted therapy. The aim of the present study was to examine the effect of hypoxia on gene expression and the changes that occur in relation to drug resistance in a human NSCLC cell line (A549). A549 cells were exposed to 72‑h hypoxic episodes (<1% oxygen) for a total of 10 episodes (acute). The alterations in gene expression were examined using PCR array technology after 10 episodes of acute hypoxia and compared with normoxic cells. The chemoresistance of hypoxic cells toward doxorubicin was measured using a MTT cell proliferation assay. A549 cells were affected by acute hypoxia leading to induced doxorubicin chemoresistance. Evident changes in the gene expression level were identified following episodes of acute hypoxia. The most important changes occurred in the estrogen receptor 1 (ESR1) and Finkel‑Biskis‑Jinkins osteosarcoma (FOS) pathways and in different nucleic transcription factors such as aryl hydrocarbon receptor and cyclin‑dependent kinase inhibitor. The present study showed that exposing cells to prolonged periods of hypoxia results in different gene expression changes. There was induction of chemo‑resistance due to acute hypoxia. ESR1 and c‑FOS are proposed as a potential hypoxia genes in lung cancer.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015.PubMed/NCBI View Article : Google Scholar

2 

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T and Thun MJ: Cancer Statistics, 2008. CA Cancer J Clin. 58:71–96. 2008.PubMed/NCBI View Article : Google Scholar

3 

Sinha BK, Mimnaugh EG, Rajagopalan S and Myers CE: Adriamycin activation and oxygen free radical formation in human breast tumor cells: Protective role of glutathione peroxidase in adriamycin resistance 1. Cancer Res. 49:3844–3848. 1989.PubMed/NCBI

4 

Vaupel P and Harrison L: Tumor hypoxia: Causative factors, compensatory mechanisms, and cellular response. Oncologist. 9 (Suppl 5):S4–S9. 2004.PubMed/NCBI View Article : Google Scholar

5 

Das B, Tsuchida R, Malkin D, Koren G, Baruchel S and Yeger H: Hypoxia enhances tumor stemness by increasing the invasive and tumorigenic side population fraction. Stem Cells. 26:1818–1830. 2008.PubMed/NCBI View Article : Google Scholar

6 

Chen Z, Han F, Du Y, Shi H and Zhou W: Hypoxic microenvironment in cancer: Molecular mechanisms and therapeutic interventions. Sig Transduct Target Ther. 8(70)2023.PubMed/NCBI View Article : Google Scholar

7 

Bayer C and Vaupel P: Acute versus chronic hypoxia in tumors : Controversial data concerning time frames and biological consequences. Strahlenther Onkol. 188:616–627. 2012.PubMed/NCBI View Article : Google Scholar

8 

Le QT, Chen E, Salim A, Cao H, Kong CS, Whyte R, Donington J, Cannon WA, Wakelee H, Tibshirani R, et al: An evaluation of tumor oxygenation and gene expression in patients with early stage non-small cell lung cancers. Clin. Cancer Res. 12:1507–1514. 2006.PubMed/NCBI View Article : Google Scholar

9 

Ziółkowska-Suchanek I: Mimicking tumor hypoxia in non-small cell lung cancer employing three-dimensional in vitro models. Cells. 10(141)2021.PubMed/NCBI View Article : Google Scholar

10 

Marhuenda E, Campillo N, Gabasa M, Martínez-García MA, Campos-Rodríguez F, Gozal D, Navajas D, Alcaraz J, Farré R and Almendros I: Effects of Sustained and Intermittent Hypoxia on Human Lung Cancer Cells. Am J Respir Cell Mol Biol. 61:540–544. 2019.PubMed/NCBI View Article : Google Scholar

11 

Rouschop KM, Ramaekers CH, Schaaf MB, Keulers TG, Savelkouls KG, Lambin P, Koritzinsky M and Wouters BG: Autophagy is required during cycling hypoxia to lower production of reactive oxygen species. Radiother Oncol. 92:411–416. 2009.PubMed/NCBI View Article : Google Scholar

12 

Hsieh CH, Shyu WC, Chiang CY, Kuo JW, Shen WC and Liu RS: NADPH oxidase subunit 4-mediated reactive oxygen species contribute to cycling hypoxia-promoted tumor progression in glioblastoma multiforme. PLoS One. 6(e23945)2011.PubMed/NCBI View Article : Google Scholar

13 

Rofstad EK, Gaustad JV, Egeland TA, Mathiesen B and Galappathi K: Tumors exposed to acute cyclic hypoxic stress show enhanced angiogenesis, perfusion and metastatic dissemination. Int J Cancer. 127:1535–1546. 2010.PubMed/NCBI View Article : Google Scholar

14 

Pires IM, Bencokova Z, Milani M, Folkes LK, Li JL, Stratford MR, Harris AL and Hammond EM: Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability. Cancer Res. 70:925–935. 2010.PubMed/NCBI View Article : Google Scholar

15 

Erler JT, Cawthorne CJ, Williams KJ, Koritzinsky M, Wouters BG, Wilson C, Miller C, Demonacos C, Stratford IJ and Dive C: Hypoxia-Mediated Down-Regulation of Bid and Bax in Tumors Occurs via Hypoxia-Inducible Factor 1-Dependent and -Independent Mechanisms and Contributes to Drug Resistance. Mol Cell Biol. 24:2875–2889. 2004.PubMed/NCBI View Article : Google Scholar

16 

Vaupel P, Kelleher DK and Höckel M: Oxygenation status of malignant tumors: Pathogenesis of hypoxia and significance for tumor therapy. Semin Oncol. 28 (2 Suppl 8):29–35. 2001.PubMed/NCBI View Article : Google Scholar

17 

Al-Najjar LM, Zihlif M and Jarrar Y: Differential molecular alterations promoting non-small cell lungcancer under hypoxia. Genome Instab Dis. 3:108–121. 2022.

18 

Chen YL, Yang TY, Chen KC, Wu CL, Hsu SL and Hsueh CM: Hypoxia can impair doxorubicin resistance of non-small cell lung cancer cells by inhibiting MRP1 and P-gp expression and boosting the chemosensitizing effects of MRP1 and P-gp blockers. Cell Oncol (Dordr). 39:411–433. 2016.PubMed/NCBI View Article : Google Scholar

19 

Chou CW, Wang CC, Wu CP, Lin YJ, Lee YC, Cheng YW and Hsieh CH: Tumor cycling hypoxia induces chemoresistance in glioblastoma multiforme by upregulating the expression and function of ABCB1. Neuro Oncol. 14:1227–1238. 2012.PubMed/NCBI View Article : Google Scholar

20 

Green SL and Giaccia AJ: Tumor hypoxia and the cell cycle: Implications for malignant progression and response to therapy. Cancer J Sci Am. 4:218–223. 1998.PubMed/NCBI

21 

Zhou SF, Wang B, Yang LP and Liu JP: Structure, function, regulation and polymorphism and the clinical significance of human cytochrome P450 1A2. Drug Metab Rev. 42:268–354. 2010.PubMed/NCBI View Article : Google Scholar

22 

AbuHammad S and Zihlif M: Gene expression alterations in doxorubicin resistant MCF7 breast cancer cell line. Genomics. 101:213–220. 2013.PubMed/NCBI View Article : Google Scholar

23 

Lei JT, Gou X, Seker S and Ellis MJ: ESR1 alterations and metastasis in estrogen receptor positive breast cancer. J Cancer Metastasis Treat. 5(38)2019.PubMed/NCBI View Article : Google Scholar

24 

Fialka I, Schwarz H, Reichmann E, Oft M, Busslinger M and Beug H: The estrogen-dependent C-junER protein causes a reversible loss of mammary epithelial cell polarity involving a destabilization of adherens junctions. J Cell Biol. 132:1115–1132. 1996.PubMed/NCBI View Article : Google Scholar

25 

Gartel AL and Tyner AL: The role of the cyclin-dependent kinase inhibitor p21 in apoptosis. Mol Cancer Ther. 1:639–649. 2002.PubMed/NCBI

26 

Bunz F, Hwang PM, Torrance C, Waldman T, Zhang Y, Dillehay L, Williams J, Lengauer C, Kinzler KW and Vogelstein B: Disruption of p53 in human cancer cells alters the responses to therapeutic agents. J Clin. Invest. 104:263–269. 1999.PubMed/NCBI View Article : Google Scholar

27 

Muhammad N, Bhattacharya S, Steele R, Phillips N and Ray RB: Involvement of c-Fos in the promotion of cancer stem-like cell properties in head and neck squamous cell carcinoma. Clin Cancer Res. 23:3120–3128. 2017.PubMed/NCBI View Article : Google Scholar

28 

Sermeus A, Genin M, Maincent A, Fransolet M, Notte A, Leclere L, Riquier H, Arnould T and Michiels C: Hypoxia-Induced modulation of apoptosis and BCL-2 family proteins in different cancer cell types. PLoS One. 7(e47519)2012.PubMed/NCBI View Article : Google Scholar

29 

Adams JM and Cory S: The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene. 26:1324–1337. 2007.PubMed/NCBI View Article : Google Scholar

30 

Brtko J: Role of retinoids and their cognate nuclear receptors in breast cancer chemoprevention. Cent Eur J Public Health. 15:3–6. 2007.PubMed/NCBI View Article : Google Scholar

31 

Hua F, Fang N, Li X, Zhu S, Zhang W and Gu J: A meta-analysis of the relationship between RARβ gene promoter methylation and non-small cell lung cancer. PLoS One. 9(e96163)2014.PubMed/NCBI View Article : Google Scholar

32 

Liu Z, Zhang L, Ding F, Li J, Guo M, Li W, Wang Y, Yu Z, Zhan Q, Wu M and Liu Z: 5-Aza-2'-deoxycytidine induces retinoic acid receptor-beta(2) demethylation and growth inhibition in esophageal squamous carcinoma cells. Cancer Lett. 230:271–283. 2005.PubMed/NCBI View Article : Google Scholar

33 

Pérez RJ, Benoit YD and Gudas LJ: Deletion of retinoic acid receptor β (RARβ) impairs pancreatic endocrine differentiation. Exp Cell Res. 319:2196–204. 2013.PubMed/NCBI View Article : Google Scholar

34 

Ren HY, Chen B, Huang GL, Liu Y and Shen DY: Upregulation of retinoic acid receptor-β reverses drug resistance in cholangiocarcinoma cells by enhancing susceptibility to apoptosis. Mol Med Rep. 14:3602–3608. 2016.PubMed/NCBI View Article : Google Scholar

35 

Li Y, Lu DG, Ma YM and Liu H: Association between Retinoic acid receptor-β hypermethylation and NSCLC risk: A meta-analysis and literature review. Oncotarget. 8:5814–5822. 2017.PubMed/NCBI View Article : Google Scholar

36 

Demchenko YN, Glebov OK, Zingone A, Keats JJ, Bergsagel PL and Kuehl WM: Classical and/or alternative NF-kappaB pathway activation in multiple myeloma. Blood. 115:3541–3552. 2010.PubMed/NCBI View Article : Google Scholar

37 

Qin H, Zhou J, Zhou P, Xu J, Tang Z, Ma H and Guo F: Prognostic significance of RelB overexpression in non-small cell lung cancer patients. Thorac. Cancer. 7:415–421. 2016.PubMed/NCBI View Article : Google Scholar

38 

Adam AP, George A, Schewe D, Bragado P, Iglesias BV, Ranganathan AC, Kourtidis A, Conklin DS and Aguirre-Ghiso JA: Computational identification of a p38SAPK-regulated transcription factor network required for tumor cell quiescence. Cancer Res. 69:5664–5672. 2009.PubMed/NCBI View Article : Google Scholar

39 

Zhao Y, Lu H, Yan A, Yang Y, Meng Q, Sun L, Pang H, Li C, Dong X and Cai L: ABCC3 as a marker for multidrug resistance in non-small cell lung cancer. Sci Rep. 3(3120)2013.PubMed/NCBI View Article : Google Scholar

40 

Fukuda Y and Schuetz JD: ABC transporters and their role in nucleoside and nucleotide drug resistance. Biochem Pharmacol. 83:1073–1083. 2012.PubMed/NCBI View Article : Google Scholar

41 

Kirovski G, Stevens AP, Czech B, Dettmer K, Weiss TS, Wild P, Hartmann A, Bosserhoff AK, Oefner PJ and Hellerbrand C: Down-regulation of methylthioadenosine phosphorylase (MTAP) induces progression of hepatocellular carcinoma via accumulation of 5'-deoxy-5'-methylthioadenosine (MTA). Am J Pathol. 178:1145–1152. 2011.PubMed/NCBI View Article : Google Scholar

42 

Chu IM, Hengst L and Slingerland JM: The Cdk inhibitor p27 in human cancer: Prognostic potential and relevance to anticancer therapy. Nat Rev Cancer. 8:253–267. 2008.PubMed/NCBI View Article : Google Scholar

43 

Semenza GL: Intratumoral hypoxia and mechanisms ofimmune evasion mediated by hypoxia-inducible factors. Physiology (Bethesda). 36:73–83. 2021.PubMed/NCBI View Article : Google Scholar

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Spandidos Publications style
Abualnadi R, Tarboush NA, Shhab M and Zihlif M: Gene expression alterations in hypoxic A549 lung cancer cell line. Biomed Rep 21: 183, 2024.
APA
Abualnadi, R., Tarboush, N.A., Shhab, M., & Zihlif, M. (2024). Gene expression alterations in hypoxic A549 lung cancer cell line. Biomedical Reports, 21, 183. https://doi.org/10.3892/br.2024.1871
MLA
Abualnadi, R., Tarboush, N. A., Shhab, M., Zihlif, M."Gene expression alterations in hypoxic A549 lung cancer cell line". Biomedical Reports 21.6 (2024): 183.
Chicago
Abualnadi, R., Tarboush, N. A., Shhab, M., Zihlif, M."Gene expression alterations in hypoxic A549 lung cancer cell line". Biomedical Reports 21, no. 6 (2024): 183. https://doi.org/10.3892/br.2024.1871
Copy and paste a formatted citation
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Spandidos Publications style
Abualnadi R, Tarboush NA, Shhab M and Zihlif M: Gene expression alterations in hypoxic A549 lung cancer cell line. Biomed Rep 21: 183, 2024.
APA
Abualnadi, R., Tarboush, N.A., Shhab, M., & Zihlif, M. (2024). Gene expression alterations in hypoxic A549 lung cancer cell line. Biomedical Reports, 21, 183. https://doi.org/10.3892/br.2024.1871
MLA
Abualnadi, R., Tarboush, N. A., Shhab, M., Zihlif, M."Gene expression alterations in hypoxic A549 lung cancer cell line". Biomedical Reports 21.6 (2024): 183.
Chicago
Abualnadi, R., Tarboush, N. A., Shhab, M., Zihlif, M."Gene expression alterations in hypoxic A549 lung cancer cell line". Biomedical Reports 21, no. 6 (2024): 183. https://doi.org/10.3892/br.2024.1871
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