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Article Open Access

Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation

  • Authors:
    • Barsha Saha
    • Shouvik Chakravarty
    • Sukanta Ray
    • Hemabha Saha
    • Kshaunish Das
    • Indranil Ghosh
    • Bibekanand Mallick
    • Nidhan K. Biswas
    • Srikanta Goswami
  • View Affiliations / Copyright

    Affiliations: Biotechnology Research and Innovation Council-National Institute of Biomedical Genomics, Kalyani, Nadia, West Bengal 741251, India, Institute of Postgraduate Medical Education & Research, Kolkata 700020, India, Chittaranjan National Cancer Institute, Kolkata 700026, India, National Institute of Technology, Rourkela 769008, India
    Copyright: © Saha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 186
    |
    Published online on: October 7, 2024
       https://doi.org/10.3892/br.2024.1874
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Abstract

The aggressiveness of pancreatic ductal adenocarcinoma is primarily due to lack of effective early detection biomarkers. Circulating non‑coding RNAs serve as diagnostic or prognostic biomarkers in multiple types of cancer. Comparison of their expression between diseased tissue and relevant body fluids such as saliva, urine, bile, pancreatic juice, blood etc. may reveal mechanistic involvement of common non‑coding RNAs. piwi‑interacting RNAs (piRNAs) are a class of non‑coding RNAs. The aim of the present study was to investigate plasma and tumour tissue piRNA changes in patients with pancreatic cancer (PC) and explore the possible role in tumorigenesis and pancreatic inflammation. Sequencing of circulating plasma small RNAs from patients with PC and chronic pancreatitis (CP) was performed and differentially expressed piRNAs were compared with those in tissues. Subsequent search for target genes for those piRNAs was performed followed by pathway and cluster analysis. A total of 36 piRNAs were shown to be deregulated in pancreatic tumour tissue and alteration of 11 piRNAs was detected in plasma of patients with PC. piRNAs hsa‑piR‑23246, hsa‑piR‑32858 and hsa‑piR‑9137 may serve a key role in PC development as their expression was correlated in both plasma and tumour tissue. Key piRNA‑target interactions interfering with key biological pathways were also characterized. A total of 19 deregulated piRNAs in plasma samples of patients with CP was identified; these targeted genes responsible for chronic inflammation. Therefore, the present study provides a comprehensive description of piRNA alteration in pancreatic malignancy and inflammation; these may be explored for biomarker potential in future.
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Copy and paste a formatted citation
Spandidos Publications style
Saha B, Chakravarty S, Ray S, Saha H, Das K, Ghosh I, Mallick B, Biswas NK and Goswami S: Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation. Biomed Rep 21: 186, 2024.
APA
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I. ... Goswami, S. (2024). Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation. Biomedical Reports, 21, 186. https://doi.org/10.3892/br.2024.1874
MLA
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I., Mallick, B., Biswas, N. K., Goswami, S."Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation". Biomedical Reports 21.6 (2024): 186.
Chicago
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I., Mallick, B., Biswas, N. K., Goswami, S."Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation". Biomedical Reports 21, no. 6 (2024): 186. https://doi.org/10.3892/br.2024.1874
Copy and paste a formatted citation
x
Spandidos Publications style
Saha B, Chakravarty S, Ray S, Saha H, Das K, Ghosh I, Mallick B, Biswas NK and Goswami S: Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation. Biomed Rep 21: 186, 2024.
APA
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I. ... Goswami, S. (2024). Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation. Biomedical Reports, 21, 186. https://doi.org/10.3892/br.2024.1874
MLA
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I., Mallick, B., Biswas, N. K., Goswami, S."Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation". Biomedical Reports 21.6 (2024): 186.
Chicago
Saha, B., Chakravarty, S., Ray, S., Saha, H., Das, K., Ghosh, I., Mallick, B., Biswas, N. K., Goswami, S."Correlating tissue and plasma‑specific piRNA changes to predict their possible role in pancreatic malignancy and chronic inflammation". Biomedical Reports 21, no. 6 (2024): 186. https://doi.org/10.3892/br.2024.1874
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