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Article Open Access

Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction

  • Authors:
    • Liyan Ye
    • Xiujuan Zheng
    • Yali Yang
    • Ying Lyu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Jinhua Maternal and Child Health Hospital, Jinhua, Zhejiang 321000, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 111
    |
    Published online on: May 6, 2025
       https://doi.org/10.3892/br.2025.1989
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Abstract

Fetal growth restriction (FGR) is associated with an increased risk of neonatal morbidity and mortality, as well as the development of metabolic syndrome in adulthood. The present study investigated the regulatory mechanisms of Notch signaling in FGR progression. The expression levels of Notch1 and Jagged1 were determined using reverse transcription‑quantitative PCR, western blotting, immunofluorescence staining and immunohistochemistry (IHC). ELISA was used to measure the concentrations of IL‑10, IL‑17 and IL‑35 in serum and placental samples. ELISA and western blotting determined the inflammation‑ and angiogenesis‑related cytokine levels. Th17, Treg and macrophage levels were determined using IHC and flow cytometry. Additionally, hematoxylin & eosin staining and TUNEL assay assessed placenta histology and trophoblast cell apoptosis. Significant trophoblast apoptosis was observed in the placenta of FGR pregnancies. The expression of Notch1 and Jagged1 in peripheral blood mononuclear cells and placental tissues of FGR pregnancies was significantly lower than in the control group. The FGR group exhibited a remarkable inflammation, anti‑angiogenesis and immune dysfunction. In conclusion, the Notch signaling pathway mediates immune balance to regulate the development of FGR. These findings offer the potential for advancing innovative predictive, diagnostic and therapeutic approaches for FGR.
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Copy and paste a formatted citation
Spandidos Publications style
Ye L, Zheng X, Yang Y and Lyu Y: Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction. Biomed Rep 23: 111, 2025.
APA
Ye, L., Zheng, X., Yang, Y., & Lyu, Y. (2025). Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction. Biomedical Reports, 23, 111. https://doi.org/10.3892/br.2025.1989
MLA
Ye, L., Zheng, X., Yang, Y., Lyu, Y."Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction". Biomedical Reports 23.1 (2025): 111.
Chicago
Ye, L., Zheng, X., Yang, Y., Lyu, Y."Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction". Biomedical Reports 23, no. 1 (2025): 111. https://doi.org/10.3892/br.2025.1989
Copy and paste a formatted citation
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Spandidos Publications style
Ye L, Zheng X, Yang Y and Lyu Y: Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction. Biomed Rep 23: 111, 2025.
APA
Ye, L., Zheng, X., Yang, Y., & Lyu, Y. (2025). Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction. Biomedical Reports, 23, 111. https://doi.org/10.3892/br.2025.1989
MLA
Ye, L., Zheng, X., Yang, Y., Lyu, Y."Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction". Biomedical Reports 23.1 (2025): 111.
Chicago
Ye, L., Zheng, X., Yang, Y., Lyu, Y."Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction". Biomedical Reports 23, no. 1 (2025): 111. https://doi.org/10.3892/br.2025.1989
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