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Effect of Gracilaria fisheri sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury

  • Authors:
    • Tawut Rudtanatip
    • Jenjiralai Phanphak
    • Somsuda Somintara
    • Jamal El-Abid
    • Kanokpan Wongprasert
    • José Kovensky
    • Waraporn Sakaew
  • View Affiliations

    Affiliations: Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Mueang, Khon Kaen 40002, Thailand, Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Svante Arrhenius Väg 16C 10691, Stockholm, Sweden, Department of Anatomy, Faculty of Science, Mahidol University, Phaya Thai, Bangkok 10400, Thailand, Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources (LG2A) CNRS UMR 7378, Université de Picardie Jules Verne, Amiens 80039, France
  • Published online on: May 22, 2025     https://doi.org/10.3892/br.2025.2001
  • Article Number: 123
  • Copyright: © Rudtanatip et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exposure to oxalate crystals causes cellular injury and dysfunction in the renal tubular epithelium. Sulfated galactan with increased sulfation (SGS) from the red seaweed Gracilaria fisheri exhibits anti‑urolithiasis effects by inhibiting oxalate crystal formation and preventing sodium oxalate (NaOX)‑induced death of renal tubular (HK‑2) cells. However, the effects of SGS on wound healing and adhesion molecule expression in NaOX‑induced HK‑2 cell injury remain unexplored. The present study investigated the effects of SGS on wound healing and the regulation of adhesion molecule expression in NaOX‑induced HK‑2 cell damage. The findings showed that SGS promoted wound healing in HK‑2 cells following a scratch injury under NaOX‑induced conditions. NaOX exposure increased the expression of CD44 and vimentin while decreasing the expression of EpCAM, E‑cadherin, occludin and ZO‑1, as demonstrated by reverse transcription‑quantitative PCR, western blotting and immunofluorescence analysis. Treatment with SGS restored these adhesion molecule expression levels to near normal. Scanning electron microscopy revealed that SGS also reversed NaOX‑induced morphological changes in HK‑2 cells. Additionally, SGS reduced the expression of Akt and p38 while upregulating PI3K and Erk1/2 in NaOX‑treated HK‑2 cells. These results suggested that SGS enhances wound healing and regulates the expression of adhesion molecules, possibly through the PI3K/Akt and MAPK (p38 and Erk1/2) signaling pathways, highlighting the potential of SGS as a promising therapeutic compound for preventing and treating NaOX‑induced renal damage.
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Copy and paste a formatted citation
Spandidos Publications style
Rudtanatip T, Phanphak J, Somintara S, El-Abid J, Wongprasert K, Kovensky J and Sakaew W: Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury. Biomed Rep 23: 123, 2025.
APA
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., & Sakaew, W. (2025). Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury. Biomedical Reports, 23, 123. https://doi.org/10.3892/br.2025.2001
MLA
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., Sakaew, W."Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury". Biomedical Reports 23.2 (2025): 123.
Chicago
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., Sakaew, W."Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury". Biomedical Reports 23, no. 2 (2025): 123. https://doi.org/10.3892/br.2025.2001
Copy and paste a formatted citation
x
Spandidos Publications style
Rudtanatip T, Phanphak J, Somintara S, El-Abid J, Wongprasert K, Kovensky J and Sakaew W: Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury. Biomed Rep 23: 123, 2025.
APA
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., & Sakaew, W. (2025). Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury. Biomedical Reports, 23, 123. https://doi.org/10.3892/br.2025.2001
MLA
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., Sakaew, W."Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury". Biomedical Reports 23.2 (2025): 123.
Chicago
Rudtanatip, T., Phanphak, J., Somintara, S., El-Abid, J., Wongprasert, K., Kovensky, J., Sakaew, W."Effect of <em>Gracilaria fisheri</em> sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate‑induced HK‑2 cell injury". Biomedical Reports 23, no. 2 (2025): 123. https://doi.org/10.3892/br.2025.2001
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