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Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia

  • Authors:
    • Jorge Organista‑Nava
    • Yazmín Gómez‑Gómez
    • Corayma Yazmín Gómez‑Sánchez
    • Eric Genaro Salmerón‑Bárcenas
    • Ma Isabel Zubillaga‑Guerrero
    • Ana Bertha Rivera‑Ramírez
    • Mónica Virginia Saavedra‑Herrera
    • Oscar Alvarez‑Palacios
    • Francisco Israel Torres‑Rojas
    • Luz Del Carmen Alarcón‑Romero
    • Berenice Illades‑Aguiar
    • Marco Antonio Leyva‑Vázquez
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Biomedicine, Faculty of Chemical and Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero 39090, Mexico, Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV‑IPN) 07360, Mexico City, Mexico, Laboratory of Cytopathology and Immunohistochemistry, Faculty of Chemical Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero 39090, Mexico, Research department, ‘Arturo Beltrán Ortega’ Cancer Institute, Acapulco, Guerrero 39570, Mexico
    Copyright: © Organista‑Nava et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 164
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    Published online on: August 12, 2025
       https://doi.org/10.3892/br.2025.2042
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Abstract

Oct3/4 is a transcription factor that maintains the stemness of both embryonic and adult stem cells. The Oct3/4 gene produces three isoforms: namely, Oct3/4A, Oct3/4B and Oct3/4B1. Increased Oct3/4 expression is associated with lower survival rates and chemoresistance in patients with cancer. The present study examined the association between Oct3/4 isoforms expression and the risk of relapse to acute lymphoblastic leukemia (ALL). The mRNA expression levels of Oct3/4A, Oct3/4B and Oct3/4B1 were analyzed using reverse transcription‑quantitative PCR in 51 patients with ALL and 12 children without ALL. The results of the present study indicated that the mRNA expression levels of Oct3/4A (P=0.008), Oct3/4B (P=0.019) and Oct3/4B1 (P=0.025) were significantly elevated in patients with ALL. Moreover, elevated mRNA expression levels of Oct3/4A [odds ratio (OR)=13.33; 95% confidence interval (CI): 1.57‑112.99; P=0.018), Oct3/4B (OR=20.00; 95% CI: 2.37‑168.64; P=0.006) and Oct3/4B1 (OR=7.26, 95% CI: 1.43‑36.93; P=0.017) isoforms were associated with a heightened risk of relapse in patients with ALL. The multivariate analysis, stratified using established prognostic factors, identified Oct3/4A, Oct3/4B and Oct3/4B1 mRNA expression levels as independent prognostic markers of ALL. The results of the present study also indicated that the expression levels of Oct3/4A (P=0.0112), Oct3/4B (P=0.0044) and Oct3/4B1 (P=0.0071) were significantly elevated in CD34+/CD38‑ patients with ALL compared with those in CD34+/CD38+ patients with ALL. Thus, increased mRNA expression levels of Oct3/4A, Oct3/4B and Oct3/4B1 may be associated with a poor prognosis in patients with ALL.
View Figures

Figure 1

Oct3/4 expression is increased in
ALL. (A) and (B) Analysis of the cBioPortal database indicated that
Oct3/4 levels were elevated in patients with ALL. The boxplot
represents log2 values on a logarithmic scale. Results obtained
from the GEPIA database confirmed that Oct3/4 expression was
elevated in patients with ALL. All samples used in this analysis
were sourced from the Cancer Genome Atlas dataset. ALL, acute
lymphoblastic leukemia; GEPIA, Gene Expression Profiling
Interactive Analysis.

Figure 2

Expression patterns of Oct3/4A,
Oct3/4B and Oct3/4B1 in patients with ALL. The mRNA expression
levels of Oct3/4 isoforms were significantly higher in patients
with ALL compared with healthy individuals. (A) Oct3/4A, median
(25-75th percentiles) at 8.55 (0.32-46.86; P=0.008). (B) Oct3/4B,
6.62 (0.52-40.83; P=0.019). C) Oct3/4B1, 14.42 (0.71-81.09;
P=0.025). ALL, acute lymphoblastic leukemia.

Figure 3

Expression patterns of Oct3/4A,
Oct3/4B and Oct3/4B1 in patients with ALL, both with and without
relapse. mRNA expression levels of Oct3/4 isoforms were
significantly elevated in patients with ALL experiencing relapse.
Results are displayed as the median (25-75th percentiles). (A)
Oct3/4A, 25.02 (3.49-716.60; P=0.0004). (B) Oct3/4B, 20.72
(3.52-245.80; P=0.0004). (C) Oct3/4B1, 35.64 (8.12-121.40;
P=0.0005). ALL, acute lymphoblastic leukemia.

Figure 4

Kaplan-Meier curves were used to
demonstrate the potential association between mRNA expression
levels of Oct3/4 isoforms and the OS of patients with ALL. (A) OS
in patients with ALL with high or low Oct3/4A expression (P=0.398).
(B) OS in patients with ALL with high or low Oct3/4B expression
(P=0.983). (C) OS in patients with ALL with high or low Oct3/4B1
expression (P=0.924). OS, overall survival; ALL, acute
lymphoblastic leukemia.

Figure 5

Expression patterns of Oct3/4A,
Oct3/4B and Oct3/4B1 in CD34+ and CD34-
patients with ALL. The mRNA expression levels of Oct3/4 isoforms
were significantly elevated in CD34+ patients with ALL
(P<0.05). Results are displayed as the median (25-75th
percentiles). (A) Oct3/4A, 13.41 (1.55-83.40; P=0.0368). (C)
Oct3/4B, 9.76 (1.18-245.80; P=0.0484). (E) Oct3/4B1, 21.79
(2.89-122.40; P=0.0389). In CD34+/CD38-
patients with ALL, the expression levels of Oct3/4 isoforms were
significantly elevated (P<0.05). (B) Oct3/4A, 50.25
(10.90-166.50; P=0.0112). (D) Oct3/4B, 48.49 (4.97-133.40;
P=0.0044). (F) Oct3/4B1, 87.33 (16.64-337.50; P=0.071). Statistical
significance was determined at P<0.05. A Mann-Whitney test was
utilized to evaluate the differences in mRNA levels. ALL, acute
lymphoblastic leukemia.
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Copy and paste a formatted citation
Spandidos Publications style
Organista‑Nava J, Gómez‑Gómez Y, Gómez‑Sánchez CY, Salmerón‑Bárcenas EG, Zubillaga‑Guerrero M, Rivera‑Ramírez A, Saavedra‑Herrera M, Alvarez‑Palacios O, Torres‑Rojas F, Alarcón‑Romero L, Alarcón‑Romero L, et al: Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia. Biomed Rep 23: 164, 2025.
APA
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C.Y., Salmerón‑Bárcenas, E.G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A. ... Leyva‑Vázquez, M. (2025). Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia. Biomedical Reports, 23, 164. https://doi.org/10.3892/br.2025.2042
MLA
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C. Y., Salmerón‑Bárcenas, E. G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A., Saavedra‑Herrera, M., Alvarez‑Palacios, O., Torres‑Rojas, F., Alarcón‑Romero, L., Illades‑Aguiar, B., Leyva‑Vázquez, M."Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia". Biomedical Reports 23.4 (2025): 164.
Chicago
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C. Y., Salmerón‑Bárcenas, E. G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A., Saavedra‑Herrera, M., Alvarez‑Palacios, O., Torres‑Rojas, F., Alarcón‑Romero, L., Illades‑Aguiar, B., Leyva‑Vázquez, M."Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia". Biomedical Reports 23, no. 4 (2025): 164. https://doi.org/10.3892/br.2025.2042
Copy and paste a formatted citation
x
Spandidos Publications style
Organista‑Nava J, Gómez‑Gómez Y, Gómez‑Sánchez CY, Salmerón‑Bárcenas EG, Zubillaga‑Guerrero M, Rivera‑Ramírez A, Saavedra‑Herrera M, Alvarez‑Palacios O, Torres‑Rojas F, Alarcón‑Romero L, Alarcón‑Romero L, et al: Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia. Biomed Rep 23: 164, 2025.
APA
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C.Y., Salmerón‑Bárcenas, E.G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A. ... Leyva‑Vázquez, M. (2025). Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia. Biomedical Reports, 23, 164. https://doi.org/10.3892/br.2025.2042
MLA
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C. Y., Salmerón‑Bárcenas, E. G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A., Saavedra‑Herrera, M., Alvarez‑Palacios, O., Torres‑Rojas, F., Alarcón‑Romero, L., Illades‑Aguiar, B., Leyva‑Vázquez, M."Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia". Biomedical Reports 23.4 (2025): 164.
Chicago
Organista‑Nava, J., Gómez‑Gómez, Y., Gómez‑Sánchez, C. Y., Salmerón‑Bárcenas, E. G., Zubillaga‑Guerrero, M., Rivera‑Ramírez, A., Saavedra‑Herrera, M., Alvarez‑Palacios, O., Torres‑Rojas, F., Alarcón‑Romero, L., Illades‑Aguiar, B., Leyva‑Vázquez, M."Expression of Oct3/4 isoforms and its association with the risk of relapse in acute lymphoblastic leukemia". Biomedical Reports 23, no. 4 (2025): 164. https://doi.org/10.3892/br.2025.2042
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