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Exon 4 and intron 4 TP53 are both methylated in advanced‑stage ovarian carcinomas

  • Authors:
    • Wiktor Szewczuk
    • Oksana Szewczuk
    • Krzysztof Czajkowski
    • Robert Gromadka
    • Maciej Waledziak
    • Marek Semczuk
    • Andrzej Semczuk
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Military Institute of Medicine, 04‑141 Warsaw, Poland, IInd Department of Obstetrics and Gynecology, Warsaw Medical University, 00‑315 Warsaw, Poland, Laboratory of DNA Sequencing and Oligonucleotide Synthesis, Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02‑106 Warsaw, Poland, Department of Surgery, Military Institute of Medicine, 04‑141 Warsaw, Poland, IInd Department of Gynecological Surgery and Gynecological Oncology, Lublin Medical University, 20‑950 Lublin, Poland
    Copyright: © Szewczuk et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 176
    |
    Published online on: September 12, 2025
       https://doi.org/10.3892/br.2025.2054
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Abstract

Although intragenic CpG dinucleotides are conserved during evolution, they are also sensitive to methylation‑dependent mechanisms. Methylation status of the TP53 introns 1, 3 and 4 have been analysed in stage III ovarian carcinoma (OC). In the present study, the methylation of exon 4 (10 CpG pairs) was analysed in advanced‑stage OC to investigate TP53 methylation and compare exon and intron 4 methylation patterns. A total of 80 samples from patients with advanced‑stage OC and metastatic lesions were examined, along with 80 samples derived from healthy patients who had never been diagnosed with cancer. Methylation analysis of the human A2780 ovarian cancer cell line was also performed. Exon and intron 4 were methylated in OC, corresponding metastases and paired healthy tissue. The DNA from the human A2780 ovarian cancer cell line and the normal samples from healthy subjects was also methylated. The data indicate the existence of an intragenic mechanism of regulation of TP53 activity that involves demethylation/methylation processes. This mechanism provides the ability to alter the response from cell cycle arrest to apoptosis by manipulating only the expression of long or short p53 isoforms.
View Figures

Figure 1

Sequence of intron (yellow) and exon
4 of TP53. Red, CpG. >, forward primer; <, reverse
primer; + presence of cytosine methylation, : absence of cytosine
methylation; |, nucleotide reference.

Figure 2

TP53 exon 4 methylation in
sequencing sample of primary advanced-stage endometrioid-type
ovarian carcinoma.

Figure 3

Intragenic demethylation/methylation
mechanisms regulating TP53 activity.
View References

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Copy and paste a formatted citation
Spandidos Publications style
Szewczuk W, Szewczuk O, Czajkowski K, Gromadka R, Waledziak M, Semczuk M and Semczuk A: Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas. Biomed Rep 23: 176, 2025.
APA
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., & Semczuk, A. (2025). Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas. Biomedical Reports, 23, 176. https://doi.org/10.3892/br.2025.2054
MLA
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., Semczuk, A."Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas". Biomedical Reports 23.5 (2025): 176.
Chicago
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., Semczuk, A."Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas". Biomedical Reports 23, no. 5 (2025): 176. https://doi.org/10.3892/br.2025.2054
Copy and paste a formatted citation
x
Spandidos Publications style
Szewczuk W, Szewczuk O, Czajkowski K, Gromadka R, Waledziak M, Semczuk M and Semczuk A: Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas. Biomed Rep 23: 176, 2025.
APA
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., & Semczuk, A. (2025). Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas. Biomedical Reports, 23, 176. https://doi.org/10.3892/br.2025.2054
MLA
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., Semczuk, A."Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas". Biomedical Reports 23.5 (2025): 176.
Chicago
Szewczuk, W., Szewczuk, O., Czajkowski, K., Gromadka, R., Waledziak, M., Semczuk, M., Semczuk, A."Exon 4 and intron 4 <em>TP53</em> are both methylated in advanced‑stage ovarian carcinomas". Biomedical Reports 23, no. 5 (2025): 176. https://doi.org/10.3892/br.2025.2054
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