Sorafenib suppresses the cell cycle and induces the apoptosis of hepatocellular carcinoma cell lines in serum-free media

Tomizawa,Minoru; Shinozaki,Fuminobu; Sugiyama,Takao; Yamamoto,Shigenori; Sueishi,Makoto; Yoshida,Takanobu

doi:10.3892/etm.2010.131

September-October 2010 Volume 1 Issue 5

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September-October 2010 Volume 1 Issue 5

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Article

Sorafenib suppresses the cell cycle and induces the apoptosis of hepatocellular carcinoma cell lines in serum-free media

Authors:
- Minoru Tomizawa
- Fuminobu Shinozaki
- Takao Sugiyama
- Shigenori Yamamoto
- Makoto Sueishi
- Takanobu Yoshida
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Affiliations: Department of Gastroenterology, National Hospital Organization Shimoshizu Hospital, Chiba 284-0003, Japan, Department of Radiology, National Hospital Organization Shimoshizu Hospital, Chiba 284-0003, Japan, Department of Rheumatology, National Hospital Organization Shimoshizu Hospital, Chiba 284-0003, Japan, Department of Pediatrics, National Hospital Organization Shimoshizu Hospital, Chiba 284-0003, Japan, Department of Internal Medicine, National Hospital Organization Shimoshizu Hospital, Yotsukaido, Chiba 284-0003, Japan
Pages: 863-866
|
Published online on: July 21, 2010

https://doi.org/10.3892/etm.2010.131
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Abstract

To suppress the invasion of hepatocellular carcinoma (HCC) cells into surrounding connective tissues during metastasis, we investigated the usefulness of sorafenib. In order to search for model cell lines, cell numbers were counted to reveal cell lines with the potential to proliferate in serum-free media. Cell proliferation and cell motility were analyzed with the MTS and wound assay, respectively. 5-Bromo-2'-deoxyuridine (BrdU) labeling and mitotic and apoptotic indices were analyzed to assess the cell cycle and apoptosis. The expression levels of cyclin D1 and the cleavage of caspase-3 were analyzed by Western blotting. HLF cells exhibited growth in the serum-free medium, while the other cell lines examined did not. Sorafenib suppressed the cell proliferation and motility of the HLF cells in the serum-free media. Both indices of BrdU and mitotic potential decreased and the apoptotic index was increased in the serum-free media with sorafenib, suggesting that the cell cycle was suppressed and apoptosis was induced. The expression levels of cyclin D1 decreased and the cleavage of caspase-3 was noted in the serum-free media with sorafenib. Sorafenib may be suitable for molecular therapy to suppress the metastasis of HCC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Sorafenib suppresses the cell cycle and induces the apoptosis of hepatocellular carcinoma cell lines in serum-free media

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