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Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas

  • Authors:
    • Suely Nonogaki
    • Heloisa G.A. Campos
    • Ossamu Butugan
    • Fernando A. Soares
    • Flávia Regina Rotea Mangone
    • Humberto Torloni
    • M. Mitzi Brentani
  • View Affiliations / Copyright

    Affiliations: Instituto Adolfo Lutz, Central, Divisão de Patologia, Laboratório de Imuno-histoquímica, CEP 01246-902, São Paulo, Brasil, Hospital A.C. Camargo, CEP 01509-010, São Paulo, Brasil, Departamento de Oftalmologia e Otorrinolaringologia, Faculdade de Medicina da Universidade de São Paulo, Cerqueira César CEP 05403-010, São Paulo, Brasil, Departamento de Radiologia e Oncologia, Disciplina de Oncologia, Faculdade de Medicina da Universidade de São Paulo (LIM-24), CEP 01246-903, São Paulo, Brasil
  • Pages: 921-926
    |
    Published online on: August 26, 2010
       https://doi.org/10.3892/etm.2010.141
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Abstract

Juvenile nasopharingeal angiofibroma (JNA) is a histologically benign locally aggressive tumor characterized by irregular vessels embedded in a fibrous stroma. Excessive vascularity results in bleeding complications, and the inhibition of angiogenesis is a promising strategy for managing extensive JNA tumors. To better characterize the endothelial components of JNA, we aimed to evaluate markers of vascular differentiation and proliferation, such as friend leukemia integration-1 (FLI-1) and endoglin, lymphatic markers, including podoplanin and vascular endothelial growth factor receptor 3 (VEGFR3) and its cognate ligand VEGFC, GLUT-1, a diagnostic marker that discriminates between hemangiomas and vascular malformations, and two markers of tissue remodeling, stromelysin 3 (ST3) and secreted acid protein rich in cysteine (SPARC). Antigens were assessed immunohistochemically in vessels and stromal cells of JNA archival cases (n=22). JNA endothelial cells were positive for endoglin, VEGFC and FLI-1, whereas podoplanin and VEGFR3 were negative in all cases. Both endothelial cells and fibroblasts stained for ST3 and SPARC. GLUT-1 was investigated in JNA cases, in infantile hemangiomas (n=123) and in vascular malformations (n=135) as controls. JNAs and vascular malformations were GLUT-1-negative, while hemangiomas showed positive staining. The presence of markers of endothelial differentiation and proliferation highlighted the hyper-proliferative state of JNA vessels. The absence of podoplanin and VEGFR3 underscores their blood endothelial cell characteristic. The absence of GLUT-1 discriminates JNAs from hemangiomas. ST3 and SPARC up-regulation in endothelial cells and fibroblasts may contribute to a compensatory signaling for controlling angiogenesis. Some of these markers may eventually serve as therapeutic targets. Our results may aid in the understanding of JNA pathophysiology.
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Copy and paste a formatted citation
Spandidos Publications style
Nonogaki S, Campos HG, Butugan O, Soares FA, Rotea Mangone FR, Torloni H and Mitzi Brentani M: Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas. Exp Ther Med 1: 921-926, 2010.
APA
Nonogaki, S., Campos, H.G., Butugan, O., Soares, F.A., Rotea Mangone, F.R., Torloni, H., & Mitzi Brentani, M. (2010). Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas. Experimental and Therapeutic Medicine, 1, 921-926. https://doi.org/10.3892/etm.2010.141
MLA
Nonogaki, S., Campos, H. G., Butugan, O., Soares, F. A., Rotea Mangone, F. R., Torloni, H., Mitzi Brentani, M."Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas". Experimental and Therapeutic Medicine 1.6 (2010): 921-926.
Chicago
Nonogaki, S., Campos, H. G., Butugan, O., Soares, F. A., Rotea Mangone, F. R., Torloni, H., Mitzi Brentani, M."Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas". Experimental and Therapeutic Medicine 1, no. 6 (2010): 921-926. https://doi.org/10.3892/etm.2010.141
Copy and paste a formatted citation
x
Spandidos Publications style
Nonogaki S, Campos HG, Butugan O, Soares FA, Rotea Mangone FR, Torloni H and Mitzi Brentani M: Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas. Exp Ther Med 1: 921-926, 2010.
APA
Nonogaki, S., Campos, H.G., Butugan, O., Soares, F.A., Rotea Mangone, F.R., Torloni, H., & Mitzi Brentani, M. (2010). Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas. Experimental and Therapeutic Medicine, 1, 921-926. https://doi.org/10.3892/etm.2010.141
MLA
Nonogaki, S., Campos, H. G., Butugan, O., Soares, F. A., Rotea Mangone, F. R., Torloni, H., Mitzi Brentani, M."Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas". Experimental and Therapeutic Medicine 1.6 (2010): 921-926.
Chicago
Nonogaki, S., Campos, H. G., Butugan, O., Soares, F. A., Rotea Mangone, F. R., Torloni, H., Mitzi Brentani, M."Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas". Experimental and Therapeutic Medicine 1, no. 6 (2010): 921-926. https://doi.org/10.3892/etm.2010.141
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