Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells

  • Authors:
    • Maki Sugaya-Fukasawa
    • Toru Watanabe
    • Michiko Tamura
    • Satsuki Egashira
    • Hisashi Hisatomi
  • View Affiliations

  • Published online on: November 5, 2010     https://doi.org/10.3892/etm.2010.162
  • Pages: 85-87
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Abstract

Nerve growth factor (NGF) normally induces the differentiation of PC12 cells into a neuron-like phenotype. In this study, we found that exposure of PC12 cells to a temperature of 42˚C for 24 h significantly decreased NGF-induced neurite elongation. Glial fibrillary acidic protein (GFAP) levels were decreased when PC12 cells were exposed to the heat stress, while PC12 cells overexpressing the gene encoding GFAP showed resistance. Mock-transfected PC12 cells exposed to NGF could be cultured for 72 h at 37˚C, whereas GFAP-transfected PC12 cells exposed to NGF could be cultured for over 100 h. Our results suggest that GFAP is necessary for the long-term maintenance of cells with a neuron-like phenotype.
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January-February 2011
Volume 2 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sugaya-Fukasawa M, Watanabe T, Tamura , Egashira S and Hisatomi H: Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells. Exp Ther Med 2: 85-87, 2011.
APA
Sugaya-Fukasawa, M., Watanabe, T., Tamura, ., Egashira, S., & Hisatomi, H. (2011). Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells. Experimental and Therapeutic Medicine, 2, 85-87. https://doi.org/10.3892/etm.2010.162
MLA
Sugaya-Fukasawa, M., Watanabe, T., Tamura, ., Egashira, S., Hisatomi, H."Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells". Experimental and Therapeutic Medicine 2.1 (2011): 85-87.
Chicago
Sugaya-Fukasawa, M., Watanabe, T., Tamura, ., Egashira, S., Hisatomi, H."Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells". Experimental and Therapeutic Medicine 2, no. 1 (2011): 85-87. https://doi.org/10.3892/etm.2010.162