Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells

Sugaya-Fukasawa,Maki; Watanabe,Toru; Tamura, Michiko; Egashira,Satsuki; Hisatomi,Hisashi

doi:10.3892/etm.2010.162

January-February 2011 Volume 2 Issue 1

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January-February 2011 Volume 2 Issue 1

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Article

Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells

Authors:
- Maki Sugaya-Fukasawa
- Toru Watanabe
- Michiko Tamura
- Satsuki Egashira
- Hisashi Hisatomi
View Affiliations / Copyright

Affiliations: Laboratory of Cellular and Molecular Biochemistry, Department of Materials and Life Science, Seikei Universty, Musashino-shi, Tokyo 180-8633, Japan
Pages: 85-87
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Published online on: November 5, 2010

https://doi.org/10.3892/etm.2010.162
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Abstract

Nerve growth factor (NGF) normally induces the differentiation of PC12 cells into a neuron-like phenotype. In this study, we found that exposure of PC12 cells to a temperature of 42˚C for 24 h significantly decreased NGF-induced neurite elongation. Glial fibrillary acidic protein (GFAP) levels were decreased when PC12 cells were exposed to the heat stress, while PC12 cells overexpressing the gene encoding GFAP showed resistance. Mock-transfected PC12 cells exposed to NGF could be cultured for 72 h at 37˚C, whereas GFAP-transfected PC12 cells exposed to NGF could be cultured for over 100 h. Our results suggest that GFAP is necessary for the long-term maintenance of cells with a neuron-like phenotype.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Glial fibrillary acidic protein is one of the key factors underlying neuron-like elongation in PC12 cells

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