Lactate dehydrogenase production in hepatocytes is increased at an early stage of acute liver failure

Kotoh,Kazuhiro; Kato,Masaki; Kohjima,Motoyuki; Tanaka,Masatake; Miyazaki,Masayuki; Nakamura,Kazuhiko; Enjoji,Munechika; Nakamuta,Makoto; Takayanagi,Ryoichi

doi:10.3892/etm.2011.197

March-April 2011 Volume 2 Issue 2

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March-April 2011 Volume 2 Issue 2

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Lactate dehydrogenase production in hepatocytes is increased at an early stage of acute liver failure

Authors:
- Kazuhiro Kotoh
- Masaki Kato
- Motoyuki Kohjima
- Masatake Tanaka
- Masayuki Miyazaki
- Kazuhiko Nakamura
- Munechika Enjoji
- Makoto Nakamuta
- Ryoichi Takayanagi
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Affiliations: Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan, Department of Gastroenterology, Kyushu Medical Center, National Hospital Organization, Fukuoka 810-8563, Japan, Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan
Pages: 195-199
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Published online on: January 19, 2011

https://doi.org/10.3892/etm.2011.197
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Abstract

Although the mechanism involved in acute liver failure (ALF) has not yet been clarified, microcirculatory disturbance in the liver appears to play a pivotal role in the progression of this disease. To confirm the existence of hepatic hypoxic conditions, we evaluated the amounts of lactate dehydrogenase (LDH) in hepatocytes, since its production increases under low oxygen concentrations. Histological examination was performed in 7 patients with ALF. All 7 patients underwent a liver biopsy during the acute phase of ALF, and 4 of them underwent a second biopsy during the recovery phase. The obtained samples were immunohistochemically stained with anti-LDH5 and anti-CD-68 antibodies. As controls, we examined samples from patients with acute hepatitis, chronic hepatitis and liver cirrhosis. The production of LDH by hepatocytes and the number of CD-68 positive macrophages were markedly increased at the acute phase of ALF, and both of these effects abruptly decreased during the recovery phase. By contrast, most of the samples from the patients with chronic hepatitis and acute hepatitis showed slightly any increase in LDH staining. In cirrhotic patients, partially elevated LDH production was observed mainly around the central vein, but the staining intensity was less compared to that in ALF patients. Our findings indicate that hepatic hypoxic conditions exist in ALF at the acute phase and seem to closely correlate with macrophage overactivation in the liver. We speculate that microcirculatory disturbance may be a key process in the development and progression of ALF.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Lactate dehydrogenase production in hepatocytes is increased at an early stage of acute liver failure

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