Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice

Chaolu,Huhe; Asakawa,Akihiro; Ushikai,Miharu; Li,Ying-Xiao; Cheng,Kai-Chun; Li,Jiang-Bo; Zoshiki,Takahiro; Terashi,Mutsumi; Tanaka,Chie; Atsuchi,Kaori; Sakoguchi,Takeo; Tsai,Minglun; Amitani,Haruka; Horiuchi,Masahisa; Takeuchi,Toru; Inui,Akio

doi:10.3892/etm.2011.199

Effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels in mice

Authors:
- Huhe Chaolu
- Akihiro Asakawa
- Miharu Ushikai
- Ying-Xiao Li
- Kai-Chun Cheng
- Jiang-Bo Li
- Takahiro Zoshiki
- Mutsumi Terashi
- Chie Tanaka
- Kaori Atsuchi
- Takeo Sakoguchi
- Minglun Tsai
- Haruka Amitani
- Masahisa Horiuchi
- Toru Takeuchi
- Akio Inui
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Affiliations: Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan, Division of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan, Division of Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan
Published online on: January 19, 2011 https://doi.org/10.3892/etm.2011.199
Pages: 369-373

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Abstract

Lifestyle-related diseases are associated with overeating and lack of exercise. The purpose of this study was to investigate the effect of exercise and high-fat diet on plasma adiponectin and nesfatin levels. Mice were housed for 4 weeks in 4 groups, which included the non-exercise and normal diet (SN), exercise and normal diet (EN), non-exercise and high-fat diet (SF) and the exercise and high-fat diet (EF) group. The mice in the exercise groups were housed in cages with a running wheel and were subjected to voluntary exercise. The food intake (Kcal) of the mice in the exercise groups increased compared to that of the mice in the non-exercise groups (P<0.01). Body weight and visceral fat decreased in the mice in the EF group compared to the mice in the SF group (P<0.01 and P<0.05). The temperature of the mice in the EF group increased compared to that of the mice in the SN group (P<0.05). Blood glucose, insulin (P<0.01), cholesterol (P<0.01) and triglyceride concentrations (P<0.01) increased in the SF group compared to the normal diet groups. Furthermore, plasma insulin and cholesterol concentrations increased in the SF group compared to the exercise groups (P<0.01). Plasma adiponectin and nesfatin-1 levels in the SF group decreased compared to the SN group (P<0.05). Exercise under a high-fat diet antagonized the significant decrease in the nesfatin-1 level. Exercise together with a high-fat diet affected the plasma levels of adiponectin and nesfatin. It is therefore suggested that exercise together with a high-fat diet can affect various diseases via adiponectin and nesfatin.

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1.	Yamauchi T, Oike Y, Kamon J, et al: Increased insulin sensitivity despite lipodystrophy in Crebbp heterozygous mice. Nat Genet. 30:221–226. 2002. View Article : Google Scholar : PubMed/NCBI
2.	Hara K, Boutin P, Mori Y, et al: Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes. 51:536–540. 2002. View Article : Google Scholar : PubMed/NCBI
3.	Okamoto Y, Arita Y, Nishida M, et al: An adipocyte-derived plasma protein, adiponectin, adheres to injured vascular walls. Horm Metab Res. 32:47–50. 2000. View Article : Google Scholar : PubMed/NCBI
4.	Arita Y, Kihara S, Ouchi N, et al: Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity. Biochem Biophys Res Commun. 257:79–83. 1999. View Article : Google Scholar : PubMed/NCBI
5.	Hotta K, Funahashi T, Arita Y, et al: Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol. 20:1595–1599. 2000. View Article : Google Scholar : PubMed/NCBI
6.	Hulver MW, Zheng D, Tanner CJ, et al: Adiponectin is not altered with exercise training despite enhanced insulin action. Am J Physiol Endocrinol Metab. 283:E861–E865. 2002. View Article : Google Scholar : PubMed/NCBI
7.	Boudou P, Sobngwi E, Mauvais-Jarvis F, Vexiau P and Gautier JF: Absence of exercise-induced variations in adiponectin levels despite decreased abdominal adiposity and improved insulin sensitivity in type 2 diabetic men. Eur J Endocrinol. 149:421–424. 2003. View Article : Google Scholar
8.	Yatagai T, Nishida Y, Nagasaka S, et al: Relationship between exercise training-induced increase in insulin sensitivity and adiponectinemia in healthy men. Endocr J. 50:233–238. 2003. View Article : Google Scholar : PubMed/NCBI
9.	Oh-I S, Shimizu H, Satoh T, et al: Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature. 443:709–712. 2006. View Article : Google Scholar : PubMed/NCBI
10.	Brailoiu GC, Dun SL, Brailoiu E, Inan S, Yang J, Chang JK and Dun NJ: Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain. Endocrinology. 148:5088–5094. 2007. View Article : Google Scholar : PubMed/NCBI
11.	Kohno D, Nakata M, Maejima Y, et al: Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding. Endocrinology. 149:1295–1301. 2008. View Article : Google Scholar : PubMed/NCBI
12.	Stengel A, Goebel M, Yakubov I, et al: Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa. Endocrinology. 150:232–238. 2009. View Article : Google Scholar : PubMed/NCBI
13.	Shimizu H, Oh-I S, Hashimoto K, et al: Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism. Endocrinology. 150:662–671. 2009. View Article : Google Scholar : PubMed/NCBI
14.	Price TO, Samson WK, Niehoff ML and Banks WA: Permeability of the blood-brain barrier to a novel satiety molecule nesfatin-1. Peptides. 28:2372–2381. 2007. View Article : Google Scholar : PubMed/NCBI
15.	Cowley MA and Grove KL: To be or NUCB2, is nesfatin the answer? Cell Metab. 4:421–422. 2006. View Article : Google Scholar : PubMed/NCBI
16.	Schwartz MW, Woods SC, Porte D Jr, Seeley RJ and Baskin DG: Central nervous system control of food intake. Nature. 404:661–671. 2000.PubMed/NCBI
17.	Cox KL, Burke V, Beilin LJ and Puddey IB: A comparison of the effects of swimming and walking on body weight, fat distribution, lipids, glucose, and insulin in older women-the Sedentary Women Exercise Adherence Trial 2. Metabolism. 59:1562–1573. 2010. View Article : Google Scholar : PubMed/NCBI
18.	MacLean PS, Higgins JA, Wyatt HR, et al: Regular exercise attenuates the metabolic drive to regain weight after long-term weight loss. Am J Physiol Regul Integr Comp Physiol. 297:793–802. 2009. View Article : Google Scholar : PubMed/NCBI
19.	Michailidou Z, Carter RN, Marshall E, et al: Glucocorticoid receptor haploinsufficiency causes hypertension and attenuates hypothalamic-pituitaryadrenal axis and blood pressure adaptions to high-fat diet. FASEB J. 22:3896–3907. 2008. View Article : Google Scholar
20.	Qi Y, Takahashi N, Hileman SM, et al: Adiponectin acts in the brain to decrease body weight. Nat Med. 10:524–529. 2004. View Article : Google Scholar : PubMed/NCBI
21.	Hattori Y, Nakano Y, Hattori S, Tomizawa A, Inukai K and Kasai K: High molecular weight adiponectin activates AMPK and suppresses cytokine-induced NF-kappaB activation in vascular endothelial cells. FEBS Lett. 582:1719–1724. 2008. View Article : Google Scholar : PubMed/NCBI
22.	Li QC, Wang HY, Chen X, Guan HZ and Jiang ZY: Fasting plasma levels of nesfatin-1 in patients with type 1 and type 2 diabetes mellitus and the nutrient-related fluctuation of nesfatin-1 level in normal humans. Regul Pept. 159:72–77. 2010. View Article : Google Scholar : PubMed/NCBI
23.	Su Y, Zhang J, Tang Y, Bi F and Liu JN: The novel function of nesfatin-1: anti-hyperglycemia. Biochem Biophys Res Commun. 391:1039–1042. 2010. View Article : Google Scholar : PubMed/NCBI
24.	Foo KS, Brauner H, Ostenson CG and Broberger C: Nucleobindin-2/nesfatin in the endocrine pancreas: distribution and relationship to glycaemic state. J Endocrinol. 204:255–263. 2010. View Article : Google Scholar : PubMed/NCBI