Decreased type III collagen expression in human uterine cervix of prolapse uteri

Iwahashi,Masaaki; Muragaki,Yasuteru

doi:10.3892/etm.2011.204

Decreased type III collagen expression in human uterine cervix of prolapse uteri

Authors:
- Masaaki Iwahashi
- Yasuteru Muragaki
View Affiliations

Affiliations: Department of Obstetrics and Gynecology, Wakayama Medical University, Wakayama 641-0012, Japan, Department of Pathology, Wakayama Medical University, Wakayama 641-0012, Japan
Published online on: January 20, 2011 https://doi.org/10.3892/etm.2011.204
Pages: 271-274

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The precise mechanism of prolapse uteri is not fully understood. There is evidence to suggest that abnormalities of collagen, the main component of extracellular matrix, or its repair mechanism, may predispose women to prolapse. To investigate the characteristic structure of human uterine cervix of patients with prolapse uteri, various types of collagen expression in the uterine cervix tissues of the prolapse uteri were compared to those of normal uterine cervix. After informed consent, 36 specimens of uterine cervical tissues were obtained at the time of surgery from 16 postmenopausal women with prolapse uteri (stage III-IV by the Pelvic Organ Prolapse Quantification examination) and 20 postmenopausal women without prolapse uteri (control group). Collagens were extracted from the uterine cervix tissues by salt precipitation methods. The relative levels of various collagens were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The uterine cervix was longer in the patients with prolapse uteri than those of postmenopausal controls without prolapse uteri. The ratios of type III to type I collagen in the uterine cervical tissues were significantly decreased in the prolapse uteri, as compared to those of the postmenopausal uterine cervix without prolapse. These results suggest that decreased type III collagen expression may play an important role in determing the physiology and structure of the uterine cervix tissues of prolapse uteri.

View Figures

View References

1.	Lin CQ and Bissell MJ: Multi-faceted regulation of cell differentiation by extracellular matrix. FASEB J. 7:737–743. 1993.PubMed/NCBI
2.	Madri JA and Basson MD: Extracellular matrix-cell interactions: dynamic modulators of cell, tissue and organism structure and function. Lab Invest. 66:519–521. 1992.PubMed/NCBI
3.	Haralson MA: Extracellular matrix and growth factors: an integrated interplay controlling tissue repair and progression to disease. Lab Invest. 69:369–372. 1993.PubMed/NCBI
4.	Goepel C, Heller L, Methfessel HD and Koelbl H: Periurethral connective tissue stastus of postmenopausal women with genital prolapse with and without stress incontinence. Acta Obstet Gynecol Scand. 82:659–664. 2003. View Article : Google Scholar : PubMed/NCBI
5.	Soderberg MW, Falconer C, Bystrom B, Malmstrom A and Ekman G: Young women with genital prolapse have a low collagen concentration. Acta Obstet Gynecol Scand. 83:1193–1198. 2004. View Article : Google Scholar : PubMed/NCBI
6.	Jackson SR, Avery NC, Tarlton JF, Eckford SD, Abrams P and Bailey AJ: Changes in the metabolism of collagen in genitourinary prolapse. Lancet. 347:1658–1661. 1996. View Article : Google Scholar : PubMed/NCBI
7.	Liapis A, Bakas P, Pafiti A, Frangos-Plemenos M, Arnoyannaki N and Creatsas G: Changes of collagen type III in female patients with genuine stress incontinence and pelvic floor prolapse. Eur J Obstet Gynecol Reprod Biol. 97:76–79. 2001. View Article : Google Scholar : PubMed/NCBI
8.	Makinen J, Kahari VM, Soderstrom KO, Vuorio E and Hirvonen T: Collagen synthesis in the vaginal connective tissue of patients with and without uterine prolapse. Eur J Obstet Gynecol Reprod Biol. 24:319–325. 1987. View Article : Google Scholar : PubMed/NCBI
9.	Kokcu A, Yanik F, Cetinkaya M, Alper T, Kandemir B and Malatyalioglu E: Histopathological evaluation of the connective tissue of the vaginal fascia and the uterine ligaments in women with and without pelvic relaxation. Arch Gynecol Obstet. 226:75–78. 2002. View Article : Google Scholar
10.	Chen BH, Wen Y, Li H and Polan ML: Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 13:80–87. 2002. View Article : Google Scholar : PubMed/NCBI
11.	Takano CC, Girao MJ, Sartori MG, et al: Analysis of collagen in parametrium and vaginal apex of women with and without uterine prolapase. Int Urogynecol J Floor Dysfunct. 13:342–345. 2002. View Article : Google Scholar : PubMed/NCBI
12.	Ewies A, Al-Azzawi F and Thompson J: Changes in extracellular matrix proteins in the cardinal ligaments of post-menopausal women with or without prolapse: a computerized immunohistomorphometric analysis. Hum Reprod. 18:2189–2195. 2003. View Article : Google Scholar
13.	Moalli PA, Shand SH, Zyczynski HM, Gordy SC and Meyn LA: Remodeling of vaginal connective tissue in patients with prolapse. Obstet Gynecol. 106:953–963. 2005. View Article : Google Scholar : PubMed/NCBI
14.	Friedman D, Boyd C, Norton P and Deak S: Altered type III collagen synthesis in patients with inguinal hernia. Am Surg. 218:754–760. 1993.PubMed/NCBI
15.	Ulmsten U, Ekman G, Giertz G and Malmstrom A: Different biochemical composition of connective tissue in continent and stress incontinent women. Acta Obstet Gynecol Scand. 66:455–457. 1987. View Article : Google Scholar : PubMed/NCBI
16.	Versi E, Cardozo L, Bribcat M, Cooper D, Montgomery J and Studd J: Corelation of urethral physiology and skin collagen in postmenopausal women. Br J Obstet Gynecol. 95:147–152. 1988. View Article : Google Scholar : PubMed/NCBI
17.	Bump RC, Mattiasson A, Bo K, et al: The standardization of terminology of female pelvic organ prolapse and pelvic floor dysfunction. Am J Obstet Gynecol. 175:10–17. 1996. View Article : Google Scholar : PubMed/NCBI
18.	Kivirikko KI and Prockop DJ: Hydroxylation of proline in synthetic polypeptides with purified protocollagen hydroxylase. J Biol Chem. 242:4009–4012. 1967.PubMed/NCBI
19.	Furuto DK and Miller EJ: Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. J Biol Chem. 255:290–295. 1980.PubMed/NCBI
20.	Miller EJ and Rhodes RK: Preparation and characterization of the different types of collagen. Methods Enzymol. 82:33–64. 1982. View Article : Google Scholar
21.	Sykes B, Puddle B, Francis M and Smith R: The estimation of two collagen from human dermis by interrupted gel electrophoresis. Biochem Biophys Res Commun. 72:1472–1480. 1976. View Article : Google Scholar : PubMed/NCBI
22.	Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI
23.	Danforth DN: The fibrous nature of the human cervix, and its relation to isthmic segment in gravid and non gravid uteri. Am J Obstet Gynecol. 53:541–560. 1974.PubMed/NCBI
24.	Kitamura K, Ito A, Mori Y and Hirakawa S: Glycosaminoglycans of human uterine cervix: heparan sulfate increase with reference to cervical ripening. Biochem Med. 23:159–167. 1980. View Article : Google Scholar : PubMed/NCBI
25.	Wallis RM and Hiller K: Regulation of collagen dissolution in human cervix by oestradiol-17b and progesterone. J Reprod Fertil. 62:55–61. 1981. View Article : Google Scholar : PubMed/NCBI
26.	Sato T, Ito A, Mori Y, Yamashita K, Hayakawa T and Nagase H: Hormonal regulation of collagenolysis in uterine cervical fibroblasts. Modulation of synthesis of procollagenase, prostromelysin and tissue inhibitor metalloproteinases (TIMP) by progesterone and oestradiol 17 b Biochem J. 275:645–650. 1991.PubMed/NCBI
27.	Rajabi MR, Dodge GR, Solomon S and Poole AR: Immunochemical and immunohistochemical evidence of estrogen-mediated collagenolysis as a mechanism of cervical dilatation in the guinea pig at partrition. Endocrinology. 128:371–378. 1991. View Article : Google Scholar : PubMed/NCBI
28.	Mochizuki M, Honda T, Deguchi M, Morikawa H and Tojo S: A study of the effect of dehydroepiandrosterone sulfate on so-called cervical ripening. Acta Obstet Gynecol Scand. 57:397–401. 1978. View Article : Google Scholar : PubMed/NCBI
29.	Uldbjerg N, Forman A, Petersen L, et al: Biochemical changes of the uterus and cervix during pregnancy. Medicine of the Fetus and Mother. Reece EA, Hobbins JC, Mahoney MJ and Petrie RH: JB Lippincott Co; Philadelphia: pp. 849–868. 1992
30.	Kelly RW: Pregnancy maintenance and partrition: the role of prostaglandin in manipulating the immune and inflammatory response. Endocr Rev. 15:684–706. 1994. View Article : Google Scholar : PubMed/NCBI
31.	El Maradney E, Kanayama N, Halim A, Maehara K, Sumimoto K and Terao T: The effect of interleukin-1 in rabbit cervical ripening. Eur J Obstet Gynecol Reprod Biol. 60:75–80. 1995.PubMed/NCBI
32.	Barclay CG, Brennad JE, Kelly RW and Calder AA: Interleukin-8 production by human cervix. Am J Obstet Gynecol. 169:625–632. 1993. View Article : Google Scholar : PubMed/NCBI
33.	El Maradney E, Kanayama N, Kobayashi H, et al: The role of hyaluronic acid as a mediator and regulator of cervical ripening. Hum Reprod. 12:1080–1088. 1997.PubMed/NCBI
34.	Osmers R, Blaser J, Kuhn W and Tshesche H: Interleukin-8 synthesis and the onset of labor. Obstet Gynecol. 86:223–229. 1995. View Article : Google Scholar : PubMed/NCBI
35.	Chwalisz K, Benson M, Scholz P, Daum J, Beier HM and Hegele-Hartung C: Cervical ripening with cytokine interleukin-8, interleukin-1 beta and tumour necrosis factor alpha in guinea-pigs. Hum Reprod. 9:2173–2181. 1994.PubMed/NCBI
36.	El Maradney E, Kanayama N, Halim A, Maehara K, Sumimoto K and Terao T: Interleukin-8 induces cervical ripening in rabbits. Am J Obstet Gynecol. 171:77–83. 1994.PubMed/NCBI
37.	Garfield RE, Saade G, Buhimschi C, et al: Control and assessment of the uterus and cervix during pregnancy and labour. Hum Reprod Update. 4:673–695. 1998. View Article : Google Scholar : PubMed/NCBI
38.	Tschugguel W, Schneeberger C, Lass H, et al: Human cervical ripening is associated with an increase in cervical inducible nitric oxide synthase expression. Biol Reprod. 60:1367–1372. 1999. View Article : Google Scholar : PubMed/NCBI
39.	Buhimschi I, Ali M, Jain V, Chwalisz K and Garfield RE: Differential regulation of nitric oxide in the rat uterus and cervix during pregnancy and labour. Hum Reprod. 11:1755–1766. 1996. View Article : Google Scholar : PubMed/NCBI
40.	Chwalisz K, Shao-Qing S, Garfield RE and Beier HM: Cervical ripening in guinea pigs after a local application of nitric oxide. Hum Reprod. 12:2093–2101. 1997. View Article : Google Scholar : PubMed/NCBI
41.	Ali M, Buhimschi I, Chwalisz K and Garfield RE: Changes in expression of the nitric oxide synthase isoforms in rat uterus and cervix during pregnancy and parturition. Mol Hum Reprod. 3:995–1003. 1997. View Article : Google Scholar : PubMed/NCBI
42.	Thomson AJ, Lunan CB, Cameron AD, Cameron IT, Greer IA and Norman JE: Nitric oxide donors induce ripening of the human uterine cervix: a randomised controlled trial. Br J Obstet Gynaecol. 104:1054–1057. 1997. View Article : Google Scholar : PubMed/NCBI
43.	Drapier JC and Bouton C: Modulation by nitric oxide of metalloproteinase regulatory activities. Bioessays. 18:549–556. 1996. View Article : Google Scholar : PubMed/NCBI
44.	Aspeden RM: Collagen organization in the cervix and its relation to mechanical functions. Collagen Relat Res. 8:103–112. 1988. View Article : Google Scholar : PubMed/NCBI
45.	Kleissel HP, van der Rest M, Naftolin F, Glorieux FH and de Leon A: Collagen changes in the human uterine cervix at partruition. Am J Obstet Gynecol. 130:748–753. 1978.PubMed/NCBI
46.	Ottani V, Martini D, Franchi M, Ruggeri A and Raspanti M: Hierarchical structures in fibrillar collagen. Micron. 33:587–596. 2002. View Article : Google Scholar : PubMed/NCBI
47.	Oxlund H: Relationships between the biomechanical properties, composition and molecular structure of connective tissues. Connect Tissue Res. 15:65–72. 1986. View Article : Google Scholar : PubMed/NCBI