Association of tumor necrosis factor β genetic polymorphism and sepsis susceptibility

Delongui,Francieli; Carvalho Grion,Cíntia    Magalhães; Ehara Watanabe,Maria   Angelica; Kaminami Morimoto,Helena; Bonametti,Ana   Maria; Maeda Oda,Julie   Massayo; Kallaur,Ana   Paula; Matsuo,Tiemi; Vissoci Reiche,Edna   Maria

doi:10.3892/etm.2011.213

Association of tumor necrosis factor β genetic polymorphism and sepsis susceptibility

Authors:
- Francieli Delongui
- Cíntia Magalhães Carvalho Grion
- Maria Angelica Ehara Watanabe
- Helena Kaminami Morimoto
- Ana Maria Bonametti
- Julie Massayo Maeda Oda
- Ana Paula Kallaur
- Tiemi Matsuo
- Edna Maria Vissoci Reiche
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Affiliations: Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, Londrina State University, CEP 86.038-440, Londrina, Paraná, Brazil, Department of Clinical Medicine, Health Sciences Center, University Hospital, Londrina State University, CEP 86.038-440, Londrina, Paraná, Brazil, Molecular Genetic Laboratory, Biological Sciences Center, Londrina State University, CEP 86.038-440, Londrina, Paraná, Brazil, Department of Biostatistics, Exacts Sciences Center, Londrina State University, Londrina, Paraná, Brazil
Published online on: January 20, 2011 https://doi.org/10.3892/etm.2011.213
Pages: 349-356

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Abstract

The association of the tumor necrosis factor β (TNF-β) Nco1 genetic polymorphism with susceptibility to sepsis was evaluated in 60 consecutive patients diagnosed with sepsis and in 148 healthy blood donors. Genomic DNA was extracted from peripheral blood cells and a 782 base-pair fragment of the TNF-β gene was amplified by PCR. The PCR products were subjected to Nco1 restriction digestion and analysed by restriction fragment length polymorphism analysis. Tumor necrosis factor α (TNF-α) and the C-reactive protein (CRP) serum levels were also determined by ELISA and nephelometry, respectively. Among the septic patients, the allelic frequencies of TNFB1 and TNFB2 were 0.2833 and 0.7166, respectively, and they differed from those observed in the blood donors (p=0.0282). The TNFB2 allele frequency was higher in the septic patients than in the blood donors [odds ratio=1.65 (CI 95% 1.02-2.69), p=0.0315]. The TNF-α and CRP serum levels and the APACHE II and SOFA clinical scores did not differ in the patients with the TNFB1 or TNFB2 alleles (p>0.05). The results suggest that the TNFB2 allele is associated with susceptibility to sepsis, but it was not found to be associated with the immunological and clinical biomarkers of the disease.

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