Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Lee,Hyun-Jin; Park,Chul-Hong; Son,Hyung-U; Heo,Jin-Chul; Nam,Sung-Hee; Lee,Kwang-Gil; Yeo,Joo-Hong; Yoon,Cheol-Sik; Kim,Jong-Myeung; Shin,Yong-Kyu; Kim,Si-Oh; Lee,Sang-Han

doi:10.3892/etm.2011.214

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Authors:
- Hyun-Jin Lee
- Chul-Hong Park
- Hyung-U Son
- Jin-Chul Heo
- Sung-Hee Nam
- Kwang-Gil Lee
- Joo-Hong Yeo
- Cheol-Sik Yoon
- Jong-Myeung Kim
- Yong-Kyu Shin
- Si-Oh Kim
- Sang-Han Lee
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Affiliations: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea, Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Republic of Korea, Department of Agricutural Biology, National Advanced Institute of Science and Technology, RDA, Suwon 441-707, Republic of Korea, Mycoplus Co., Ltd., Uiwang 437-820, Republic of Korea, Bion Co., Ltd., Daegu 702-701, Republic of Korea, Department of Anesthesiology, Kyungpook National University School of Medicine, Daegu 700-412, Republic of Korea
Published online on: January 20, 2011 https://doi.org/10.3892/etm.2011.214
Pages: 357-362

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Abstract

The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.

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