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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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May-June 2011 Volume 2 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies

  • Authors:
    • Jun Dai
    • Cai Zhang
    • Zhigang Tian
    • Jian Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 487-490
    |
    Published online on: March 21, 2011
       https://doi.org/10.3892/etm.2011.240
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Abstract

Homeobox containing 1 (HMBOX1) is a novel transcription factor. However, the expression of HMBOX1 and its functions in human cancer tissues and cell lines have not been fully defined. We generated two specific monoclonal antibodies, 2A5F4 and 4A4F2, against human HMBOX1. In the present study, these two anti-HMBOX1 antibodies were used to investigate the protein expression profile of HMBOX1 in various human cancer tissues and cell lines. The results showed that HMBOX1 in kidney tissue was mainly expressed in the renal tubule; the expression level of HMBOX1 was much higher in clear-cell carcinoma of the kidney originating from the renal tubule. Additionally, high levels of HMBOX1 protein were detected not only in pancreatic cancer tissue but also in the adjacent normal tissue. Notably, the expression level of HMBOX1 in liver cancer was dramatically decreased compared with that in the adjacent normal tissue. Abnormal expression of HMBOX1 in different types of carcinoma tissues suggests that HMBOX1 may be involved in the pathobiology of tumors.
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1. 

Lawrence PA and Morata G: Homeobox genes: their function in Drosophila segmentation and pattern formation. Cell Mol Immunol. 78:181–189. 1994.PubMed/NCBI

2. 

Chen S, Saiyin H, Zeng X, et al: Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity. Cytogenet Genome Res. 114:131–136. 2006. View Article : Google Scholar : PubMed/NCBI

3. 

Zhang M, Chen S, Li Q, Ling Y, Zhang J and Yu L: Characterization of a novel human HMBOX1 splicing variant lacking the homeodomain and with attenuated transcription repressor activity. Mol Biol Rep. 37:2767–2772. 2010. View Article : Google Scholar

4. 

Dai J, Wu L, Zhang C, Zheng X, Tian Z and Zhang J: Recombinant expression of a novel human transcriptional repressor HMBOX1 and preparation of anti-HMBOX1 monoclonal antibody. Cell Mol Immunol. 6:261–268. 2009. View Article : Google Scholar : PubMed/NCBI

5. 

Tsangaridou E, Polioudaki H, Sfakianaki R, et al: Differential detection of nuclear envelope autoantibodies in primary biliary cirrhosis using routine and alternative methods. BMC Gastroenterol. 10:282010. View Article : Google Scholar

6. 

Balint JP Jr, Ikeda Y, Nagai T and Terman DS: Isolation of human and canine IgM utilizing protein A affinity chromatography. Immunol Commun. 10:533–540. 1981.PubMed/NCBI

7. 

Armendariz AD and Krauss RM: Hepatic nuclear factor 1-alpha: inflammation, genetics, and atherosclerosis. Curr Opin Lipidol. 20:106–111. 2009. View Article : Google Scholar : PubMed/NCBI

8. 

Van Wering HM, Huibregtse IL, van der Zwan SM, et al: Physical interaction between GATA-5 and hepatocyte nuclear factor-1alpha results in synergistic activation of the human lactasephlorizin hydrolase promoter. J Biol Chem. 277:27659–27667. 2002.

9. 

Sourdive DJ, Transy C, Garbay S and Yaniv M: The bifunctional DCOH protein binds to HNF1 independently of its 4-alpha-carbinolamine dehydratase activity. Nucleic Acids Res. 25:1476–1484. 1997. View Article : Google Scholar : PubMed/NCBI

10. 

Soutoglou E, Papafotiou G, Katrakili N and Talianidis I: Transcriptional activation by hepatocyte nuclear factor-1 requires synergism between multiple coactivator proteins. J Biol Chem. 275:12515–12520. 2000. View Article : Google Scholar

11. 

Dohda T, Kaneoka H, Inayoshi Y, Kamihira M, Miyake K and Iijima S: Transcriptional coactivators CBP and p300 cooperatively enhance HNF-1alpa-mediated expression of the albumin gene in hepatocytes. J Biochem. 136:313–319. 2004. View Article : Google Scholar : PubMed/NCBI

12. 

Smith SB, Gasa R, Watada H, Wang J, Grimen SC and German MS: Neurogenin3 and hepatic nuclear factor 1 cooperate in activating pancreatic expression of Pax4. J Biol Chem. 278:28254–28259. 2003. View Article : Google Scholar : PubMed/NCBI

13. 

Owen K and Hattersley AT: Maturity-onset diabetes of the young: from clinical description to molecular genetic characterization. Best Pract Res Clin Endocrinol Metab. 15:309–323. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dai J, Zhang C, Tian Z and Zhang J: Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies. Exp Ther Med 2: 487-490, 2011.
APA
Dai, J., Zhang, C., Tian, Z., & Zhang, J. (2011). Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies. Experimental and Therapeutic Medicine, 2, 487-490. https://doi.org/10.3892/etm.2011.240
MLA
Dai, J., Zhang, C., Tian, Z., Zhang, J."Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies". Experimental and Therapeutic Medicine 2.3 (2011): 487-490.
Chicago
Dai, J., Zhang, C., Tian, Z., Zhang, J."Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies". Experimental and Therapeutic Medicine 2, no. 3 (2011): 487-490. https://doi.org/10.3892/etm.2011.240
Copy and paste a formatted citation
x
Spandidos Publications style
Dai J, Zhang C, Tian Z and Zhang J: Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies. Exp Ther Med 2: 487-490, 2011.
APA
Dai, J., Zhang, C., Tian, Z., & Zhang, J. (2011). Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies. Experimental and Therapeutic Medicine, 2, 487-490. https://doi.org/10.3892/etm.2011.240
MLA
Dai, J., Zhang, C., Tian, Z., Zhang, J."Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies". Experimental and Therapeutic Medicine 2.3 (2011): 487-490.
Chicago
Dai, J., Zhang, C., Tian, Z., Zhang, J."Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies". Experimental and Therapeutic Medicine 2, no. 3 (2011): 487-490. https://doi.org/10.3892/etm.2011.240
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