Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma

Inoue,Masahisa; Roan,Cheng-Hsiung; Abe,Tomomi; Buery,Rosario  R.; Nagatsuka,Hitoshi; Katase,Naoki; Nagai,Noriyuki; Setsu,Kojun

doi:10.3892/etm.2011.277

September-October 2011 Volume 2 Issue 5

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September-October 2011 Volume 2 Issue 5

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Article

Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma

Authors:
- Masahisa Inoue
- Cheng-Hsiung Roan
- Tomomi Abe
- Rosario R. Buery
- Hitoshi Nagatsuka
- Naoki Katase
- Noriyuki Nagai
- Kojun Setsu
View Affiliations / Copyright

Affiliations: Laboratories for Structure and Function Research, Faculty of Pharmaceutical Science, Tokushima Bunri University, Tokushima 770-8514, Japan, Tung's Taichung Metro Harbor Hospital, Taiwan, R.O.C., Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan
Pages: 793-797
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Published online on: May 31, 2011

https://doi.org/10.3892/etm.2011.277
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Abstract

Lymph node metastasis is considered a factor in determining the prognosis of squamous cell carcinoma (SCC). Both oral and cervical SCC tumor cells prefer lymph vessels as the route of metastasis. D2-40 is a specific marker of lymphatic endothelial cells. This study clarifies the distribution and characteristics of lymphatic vessels in oral and cervical SCCs. Immunohistochemistry was performed in 20 oral and 20 cervical SCCs (10 non-metastatic and 10 metastatic to lymph nodes) using D2-40, CD31, CD34, CD105 and double staining with D2-40 and keratin. Lymphatic vessel density (LVD) was also determined morphologically. Results showed that lymphatic vessels in both types of SCCs were distributed mainly at the superficial region beneath the epithelium. The LVD in each tumor was significantly higher compared to the corresponding normal mucosa. Moreover, the LVD in lymph node metastasis in each tumor was significantly higher compared to their non-metastatic counterparts. Cancer cell invasion was observed in the lymphatic vessels suggesting the existence of lymph node involvement during metastasis. The new lymphatic vessels that proliferated around the cancer nests in both SCCs have endothelial cell characteristics inferred to be associated with early lymphatic development and initial dissemination of cancer cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Localization and characterization of lymphatic vessels in oral and cervical squamous cell carcinoma

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Abstract

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