Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Kenan Kinaci,M.; Erkasap,Nilufer; Kucuk,Aysegul; Koken,Tulay; Tosun,Murat

doi:10.3892/etm.2011.382

Effects of quercetin on apoptosis, NF-κB and NOS gene expression in renal ischemia/reperfusion injury

Authors:
- M. Kenan Kinaci
- Nilufer Erkasap
- Aysegul Kucuk
- Tulay Koken
- Murat Tosun
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Affiliations: Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Eskisehir 26480, Turkey, Department of Physiology, Kutahya Dumlupinar University Medical Faculty, Kutahya, Turkey, Department of Biochemistry, Afyon Kocatepe University Medical Faculty, Afyon, Turkey, Department of Histology and Embryology, Afyon Kocatepe University Medical Faculty, Afyon, Turkey
Published online on: November 16, 2011 https://doi.org/10.3892/etm.2011.382
Pages: 249-254

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Abstract

The aim of this study was to investigate the effects of quercetin on nitric oxide synthase (NOS), nuclear factor-κB (NF-κB) and apoptosis in renal ischemia/reperfusion (I/R) injury in rats. A total of 42 Sprague-Dawley rats were divided into three groups. The control, I/R and I/R+quercetin (I/R+Q) groups were treated with quercetin (50 mg/kg intraperitoneal) 1 h prior to the induction of ischemia. Tissue malondialdehyde (MDA) and glutathione (GSH) levels were determined by high‑performance liquid chromatography (HPLC). p53, endothelial NOS (eNOS) and NF-κB expression were assessed immunohistochemically, and apoptosis assesment was performed using terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay. The mRNA levels of inducible NOS (iNOS) in renal tissue were determined by real-time polymerase chain reaction (RT-PCR). MDA levels were significantly decreased in the quercetin group compared to the I/R group. However, GSH levels were significantly increased with quercetin treatment in the I/R group. Histological results, the number of apoptotic and p53-positive cells, NF-κB and eNOS expression levels were significantly decreased in the quercetin treatment group compared to the I/R group. iNOS gene expression increased in the I/R group, but no significant difference was found between the I/R and quercetin treatment groups. Therefore, quercetin not only has antioxidant and anti-apoptotic activities, but also has an inhibitory effect on eNOS and NF-κB for renal tissue protection during I/R injury in rats. Therefore, quercetin may be a promising renoprotective therapeutic agent.

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