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Article

Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions

  • Authors:
    • Takehiro Ichijima
    • Kenichi Matsuzaka
    • Morio Tonogi
    • Gen-Yuki Yamane
    • Takashi Inoue
  • View Affiliations / Copyright

    Affiliations: Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental College, Ichikawa General Hospital, Ichikawa-shi, Chiba 272-8513, Japan, Oral Health Science Center hrc7, Tokyo Dental College, Chiba 261-8502, Japan
  • Pages: 165-170
    |
    Published online on: November 28, 2011
       https://doi.org/10.3892/etm.2011.393
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Abstract

The aim of the present study was to investigate the osteogenic capability of rat calvarial periosteal cells in hypoxic conditions in vitro. Periosteum was obtained from the calvarial bone of Sprague-Dawley rats. Following primary tissue culture, subcultured cells were used in hypoxic or normal conditions. On days 1, 2, 3 and 4 following the cell culture, cell proliferation and mRNA and protein expression levels were evaluated. No significant difference in the cell proliferation rate was found between the normal and hypoxic condition groups. The hypoxic condition group exhibited a stronger expression of hypoxia-inducible factor (HIF)1α, vascular endothelial growth factor (VEGF), Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN) and periostin at the mRNA level compared to that of the normal condition group. The hypoxic condition group also exhibited a stronger expression of HIF1α, VEGF, bone morphogenetic protein (BMP)2, Runx2, ALP and BSP at the protein level compared to that of the normal condition group. In conclusion, periosteal cells cultured in hypoxic conditions demonstrated activated osteogenic capability in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Ichijima T, Matsuzaka K, Tonogi M, Yamane G and Inoue T: Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions. Exp Ther Med 3: 165-170, 2012.
APA
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., & Inoue, T. (2012). Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions. Experimental and Therapeutic Medicine, 3, 165-170. https://doi.org/10.3892/etm.2011.393
MLA
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., Inoue, T."Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions". Experimental and Therapeutic Medicine 3.2 (2012): 165-170.
Chicago
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., Inoue, T."Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions". Experimental and Therapeutic Medicine 3, no. 2 (2012): 165-170. https://doi.org/10.3892/etm.2011.393
Copy and paste a formatted citation
x
Spandidos Publications style
Ichijima T, Matsuzaka K, Tonogi M, Yamane G and Inoue T: Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions. Exp Ther Med 3: 165-170, 2012.
APA
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., & Inoue, T. (2012). Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions. Experimental and Therapeutic Medicine, 3, 165-170. https://doi.org/10.3892/etm.2011.393
MLA
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., Inoue, T."Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions". Experimental and Therapeutic Medicine 3.2 (2012): 165-170.
Chicago
Ichijima, T., Matsuzaka, K., Tonogi, M., Yamane, G., Inoue, T."Osteogenic differences in cultured rat periosteal cells under hypoxic and normal conditions". Experimental and Therapeutic Medicine 3, no. 2 (2012): 165-170. https://doi.org/10.3892/etm.2011.393
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