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Article

Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro

  • Authors:
    • Zhenchuan Song
    • Chenjun Guo
    • Yong Li
    • Bibo Tan
    • Liqiao Fan
    • Jianwei Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
  • Pages: 319-323
    |
    Published online on: November 28, 2011
       https://doi.org/10.3892/etm.2011.394
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Abstract

T cell-mediated antitumor immunity is a cellular immune response that requires two signals. The dendritic cell (DC) has been considered as the most efficient antigen‑presenting cell (APC). It plays essential roles in the induction, regulation and maintenance of antitumor immunity in humans. The 4-1BB/4-1BB ligand (4-1BBL) pathway plays crucial roles in immune response, tumor immunity and autoimmune diseases through transduction of T cell co-stimulatory signals. The aim of this study was to generate the preparation protocol for a DC vaccine transfected with gastric cancer cell total ribonucleic acid (RNA) carrying the 4-1 BBL gene in vitro and to investigate its antitumor effects in murine forestomach carcinoma (MFC). The vaccine was prepared by transfecting MFC total RNAs carrying the 4-1BBL gene into the DCs that were isolated from 615 mouse bones. The T cell proliferation rate in the MFC/4‑1BBL/DC group was higher than that in the DC group. The tumor cell kill rate in the MFC/4-1BBL/DC group was higher than that in the DC group. ELISA analysis showed that IL-12 and IFN-γ in the MFC/4‑1BBL/DC group were more highly expressed compared to the other group. Collectively, our data demonstrate that the DC vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene has a stronger ability to kill gastric cancer cells through promoting T cell proliferation and enhancing the ability of cytotoxic T lymphocytes (CTLs) to kill gastric carcinoma cells and to secrete IL-12 and IFN-γ. Our results provide an effective therapeutic strategy for treating gastric cancer using a DC vaccine.
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1. 

SW LeeY ParkT SoBS KwonH CheroutreRS MittlerM CroftIdentification of regulatory functions for 4-1BB and 4-1BBL in myelopoiesis and the development of dendritic cellsNat Immunol9917926200818604213

2. 

C Reis e SousaDendritic cells in a mature ageNat Rev Immunol6476483200616691244

3. 

AK Thomas-KaskelH VeelkenActive immunotherapy of prostate cancer with a focus on dendritic cellsActas Urol Esp31668679200717896564

4. 

J BanchereauAK PaluckaDendritic cells as therapeutic vaccines against cancerNat Rev Immunol5296306200515803149

5. 

JS YuG LiuH YingWH YongKL BlackCJ WheelerVaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant gliomaCancer Res6449734979200410.1158/0008-5472.CAN-03-350515256471

6. 

T ChanA SamiA El-GayedX GuoJ XiangHER-2/ neu-gene engineered dendritic cell vaccine stimulates stronger HER-2/neu-specific immune responses compared to DNA vaccinationGene Ther1313911402200610.1038/sj.gt.330279716724093

7. 

E HuarteJR Cubillos-RuizYC NesbethUK ScarlettDG MartinezRJ BuckanovichF BenenciaRV StanT KelerP SarobeCL SentmanJR Conejo-GarciaDepletion of dendritic cells delays ovarian cancer progression by boosting antitumor immunityCancer Res6876767684200818768667

8. 

JE Moyron-QuirozJ Rangel-MorenoL HartsonK KusserMP TigheKD KlonowskiL LefrançoisLS CauleyAG HarmsenFE LundTD RandallPersistence and responsiveness of immunologic memory in the absence of secondary lymphoid organsImmunity25643654200617045819

9. 

GK KoskiPA CohenRE RosesS XuBJ CzernieckiReengineering dendritic cell-based anti-cancer vaccinesImmunol Rev222256276200818364007

10. 

KR JordanRH McMahanCB KemmlerJW KapplerJE SlanskyPeptide vaccines prevent tumor growth by activating T cells that respond to native tumor antigensProc Natl Acad Sci USA10747884789201020133772

11. 

P KrauseM BrucknerC UermösiE SingerM GroettrupDF LeglerProstaglandin E(2) enhances T-cell proliferation by inducing the costimulatory molecules OX40L, CD70, and 4-1BBL on dendritic cellsBlood11324512460200910.1182/blood-2008-05-15712319029446

12. 

KH YiH NechushtanWJ BowersGR WalkerY ZhangDG PhamER PodackHJ FederoffKA TolbaJD RosenblattAdoptively transferred tumor-specific T cells stimulated ex vivo using herpes simplex virus amplicons encoding 4-1BBL persist in the host and show antitumor activity in vivoCancer Res671002710037200710.1158/0008-5472.CAN-06-2391

13. 

Y KuangX WengX LiuH ZhuZ ChenB JiangH ChenAnti-tumor immune response induced by dendritic cells transduced with truncated PSMA IRES 4-1BBL recombinant adenovirusesCancer Lett293254262201010.1016/j.canlet.2010.01.01120149524

14. 

KA Shafer-WeaverSK WatkinsMJ AndersonLJ DraperA MalyguineWG AlvordNM GreenbergAA HurwitzImmunity to murine prostatic tumors: continuous provision of T-cell help prevents CD8 T-cell tolerance and activates tumor-infiltrating dendritic cellsCancer Res6962576264200919622771

15. 

M Habib-AgahiTT PhanPF SearleCo-stimulation with 4-1BB ligand allows extended T-cell proliferation, synergizes with CD80/CD86 and can reactivate anergic T cellsInt Immunol1913831394200710.1093/intimm/dxm10617977894

16. 

S RadhakrishnanKR WiehagenV PulkoV Van KeulenWA FaubionKL KnutsonLR PeaseInduction of a Th1 response from Th2-polarized T cells by activated dendritic cells: dependence on TCR:peptide-MHC interaction, ICAM-1, IL-12, and IFN-gammaJ Immunol17835833592200710.4049/jimmunol.178.6.3583

17. 

EL GautierT HubyF Saint-CharlesB OuzilleauJ PiraultV DeswaerteF GinhouxER MillerJL WitztumMJ ChapmanP LesnikConventional dendritic cells at the crossroads between immunity and cholesterol homeostasis in atherosclerosisCirculation11923672375200910.1161/CIRCULATIONAHA.108.80753719380622

18. 

ML DrakesSJ CzinnTG BlanchardRegulation of murine dendritic cell immune responses by Helicobacter felis antigenInfect Immun7446244633200610.1128/IAI.00289-0616861650

19. 

MR JenkinsJ MinternNL La GrutaK KedzierskaPC DohertySJ TurnerCell cycle-related a quisition of cytotoxic mediators defines the progressive differentiation to effector status for virus specific CD8+ T cellsJ Immunol18138183822200810.4049/jimmunol.181.6.381818768835

20. 

RK SharmaKG ElpekES YolcuRH SchabowskyH ZhaoL Bandura-MorganH ShirwanCostimulation as a platform for the development of vaccines: a peptide-based vaccine containing a novel form of 4-1BB ligand eradicates established tumorsCancer Res6943194326200910.1158/0008-5472.CAN-08-314119435920

21. 

T KurodaY KitadaiS TanakaX YangN MukaidaM YoshiharaK ChayamaMonocyte chemoattractant protein-1 transfection induces angiogenesis and tumorigenesis of gastric carcinoma in nude mice via macrophage recruitmentClin Cancer Res1176297636200510.1158/1078-0432.CCR-05-0798

22. 

SJ XieQH YanBE ShanZX FuFJ MengBD LiJH CaiIn vitro experimental study individualised immunotherapy induced by dendritic cells transfected with total RNA of autologous gastric cancer cellsXi Bao Yu Fen Zi Mian Yi Xue Za Zhi2292952006

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Copy and paste a formatted citation
Spandidos Publications style
Song Z, Guo C, Li Y, Tan B, Fan L and Xiao J: Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro. Exp Ther Med 3: 319-323, 2012.
APA
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., & Xiao, J. (2012). Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro. Experimental and Therapeutic Medicine, 3, 319-323. https://doi.org/10.3892/etm.2011.394
MLA
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., Xiao, J."Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro". Experimental and Therapeutic Medicine 3.2 (2012): 319-323.
Chicago
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., Xiao, J."Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro". Experimental and Therapeutic Medicine 3, no. 2 (2012): 319-323. https://doi.org/10.3892/etm.2011.394
Copy and paste a formatted citation
x
Spandidos Publications style
Song Z, Guo C, Li Y, Tan B, Fan L and Xiao J: Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro. Exp Ther Med 3: 319-323, 2012.
APA
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., & Xiao, J. (2012). Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro. Experimental and Therapeutic Medicine, 3, 319-323. https://doi.org/10.3892/etm.2011.394
MLA
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., Xiao, J."Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro". Experimental and Therapeutic Medicine 3.2 (2012): 319-323.
Chicago
Song, Z., Guo, C., Li, Y., Tan, B., Fan, L., Xiao, J."Enhanced antitumor effects of a dendritic cell vaccine transfected with gastric cancer cell total RNA carrying the 4-1BBL gene in vitro". Experimental and Therapeutic Medicine 3, no. 2 (2012): 319-323. https://doi.org/10.3892/etm.2011.394
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