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Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model

  • Authors:
    • Yanglin Gu
    • Wenhui Zhu
    • Yuedong Hao
    • Liangyu Lu
    • Yang Chen
    • Yubin Wang

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  • Published online on: December 1, 2011     https://doi.org/10.3892/etm.2011.403
  • Pages: 293-298
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Abstract

Defects of the meniscus greatly alter knee function and predispose the joint to degenerative changes. The purpose of this study was to test a recently developed cell-scaffold combination for the repair of a critical-size defect in the canine medial meniscus. A bilateral, complete resection of the anterior horn of the medial meniscus was performed in 18 Beagle canines. A PLGA scaffold was implanted into the defect of one knee of 6 canines and the contralateral defect was left untreated. Scaffolds loaded with autologous myoblasts and cultured in a chondrogenic medium for 14 days were implanted in a second series of 12 canines. Empty scaffolds were implanted in the contralateral knees. Menisci were harvested at 12 weeks. Untreated defects had a muted fibrous healing response. Defects treated with cell-free implants also showed predominantly fibrous tissue, whereas fibrocartilage was present in several scaffolds. The thickness of the repair tissue after treatment with cell-free scaffolds was significantly greater compared to the controls (p<0.05). Pre-cultured implants integrated with the host tissue, and 9 of 12 contained meniscus-like fibrocartilage when compared to 2 of the 12 controls (p<0.05). The thickness of the pre-cultured implant repair tissue was greater compared to the controls (p<0.05). This study demonstrates the repair of a critical size meniscal defect using a stem cell and scaffold-based tissue engineering approach.
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February 2012
Volume 3 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gu Y, Zhu W, Hao Y, Lu L, Chen Y and Wang Y: Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model. Exp Ther Med 3: 293-298, 2012
APA
Gu, Y., Zhu, W., Hao, Y., Lu, L., Chen, Y., & Wang, Y. (2012). Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model. Experimental and Therapeutic Medicine, 3, 293-298. https://doi.org/10.3892/etm.2011.403
MLA
Gu, Y., Zhu, W., Hao, Y., Lu, L., Chen, Y., Wang, Y."Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model". Experimental and Therapeutic Medicine 3.2 (2012): 293-298.
Chicago
Gu, Y., Zhu, W., Hao, Y., Lu, L., Chen, Y., Wang, Y."Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model". Experimental and Therapeutic Medicine 3, no. 2 (2012): 293-298. https://doi.org/10.3892/etm.2011.403