Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model

Gu,Yanglin; Zhu,Wenhui; Hao,Yuedong; Lu,Liangyu; Chen,Yang; Wang,Yubin

doi:10.3892/etm.2011.403

February 2012 Volume 3 Issue 2

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February 2012 Volume 3 Issue 2

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Article

Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model

Authors:
- Yanglin Gu
- Wenhui Zhu
- Yuedong Hao
- Liangyu Lu
- Yang Chen
- Yubin Wang
View Affiliations / Copyright

Affiliations: Department of Sports Medicine, Dongfang Hospital Affiliated to Tongji University, Shanghai 200120, P.R. China
Pages: 293-298
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Published online on: December 1, 2011

https://doi.org/10.3892/etm.2011.403
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Abstract

Defects of the meniscus greatly alter knee function and predispose the joint to degenerative changes. The purpose of this study was to test a recently developed cell-scaffold combination for the repair of a critical-size defect in the canine medial meniscus. A bilateral, complete resection of the anterior horn of the medial meniscus was performed in 18 Beagle canines. A PLGA scaffold was implanted into the defect of one knee of 6 canines and the contralateral defect was left untreated. Scaffolds loaded with autologous myoblasts and cultured in a chondrogenic medium for 14 days were implanted in a second series of 12 canines. Empty scaffolds were implanted in the contralateral knees. Menisci were harvested at 12 weeks. Untreated defects had a muted fibrous healing response. Defects treated with cell-free implants also showed predominantly fibrous tissue, whereas fibrocartilage was present in several scaffolds. The thickness of the repair tissue after treatment with cell-free scaffolds was significantly greater compared to the controls (p<0.05). Pre-cultured implants integrated with the host tissue, and 9 of 12 contained meniscus-like fibrocartilage when compared to 2 of the 12 controls (p<0.05). The thickness of the pre-cultured implant repair tissue was greater compared to the controls (p<0.05). This study demonstrates the repair of a critical size meniscal defect using a stem cell and scaffold-based tissue engineering approach.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Repair of meniscal defect using an induced myoblast-loaded polyglycolic acid mesh in a canine model

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