γ-secretase inhibition combined with cisplatin enhances apoptosis of nasopharyngeal carcinoma cells

Zhou,Jun-Xu; Han,Ji-Bo; Chen,Shi-Ming; Xu,Yu; Kong,Yong-Gang; Xiao,Bo-Kui; Tao,Ze-Zhang

doi:10.3892/etm.2011.410

γ-secretase inhibition combined with cisplatin enhances apoptosis of nasopharyngeal carcinoma cells

Authors:
- Jun-Xu Zhou
- Ji-Bo Han
- Shi-Ming Chen
- Yu Xu
- Yong-Gang Kong
- Bo-Kui Xiao
- Ze-Zhang Tao
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Affiliations: Department of Otolaryngology – Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, P.R. China
Published online on: December 6, 2011 https://doi.org/10.3892/etm.2011.410
Pages: 357-361

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Abstract

The Notch signaling pathway plays an important role in the proliferation and differentiation of cells. Although recent studies have shown that Notch plays a role in the mechanisms of cisplatin resistance, the mechanism by which Notch plays roles in intrinsic or acquired cisplatin resistance remains unclear. In the present study, poorly differentiated nasopharyngeal carcinoma cells were treated with a γ-secretase inhibitor (DAPT), which led to a decrease in the Notch intracellular domain and inhibition of Notch signaling. Treatment was not sufficient to induce pronounced apoptosis of CNE-2 cells, but did result in the down-regulation of the P-glycoprotein and ERCC1 protein. In contrast, the combined treatment of DAPT and cisplatin induced substantial cell apoptosis compared to cisplatin treatment alone.

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