Investigation of thrombospondin-1 and transforming growth factor-β expression in the heart of aging mice

Cai,Huabo; Yuan,Zhanggen; Fei,Qing; Zhao,Jiayao

doi:10.3892/etm.2011.426

March 2012 Volume 3 Issue 3

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March 2012 Volume 3 Issue 3

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Article

Investigation of thrombospondin-1 and transforming growth factor-β expression in the heart of aging mice

Authors:
- Huabo Cai
- Zhanggen Yuan
- Qing Fei
- Jiayao Zhao
View Affiliations / Copyright

Affiliations: Department of ICU, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, P.R. China, Department of Anatomy, School of Medicine, Zhejiang University City College, Hangzhou 310015, P.R. China
Pages: 433-436
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Published online on: December 20, 2011

https://doi.org/10.3892/etm.2011.426
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Abstract

Elderly patients face the problems of morbidity and mortality due to age-mediated disabilities. The purpose of the present study was to investigate the expression of thrombospondin-1 (TSP-1) and transforming growth factor-β (TGF-β) in aging mice, and its probable mechanism in the pathological changes of aging myocardium. The aging model group (AM) comprised 30-month-old mice, while the control group comprised 2-month-old mice. The pathological changes were explored by H&E staining, and the contents of superoxide dismulase (SOD) and malondialdehyde (MDA) in the hearts were determined by xanthine oxidation or TBA colorimetry. TSP-1 and TGF-β expression in the left ventricular myocardium was also measured by immunohistochemistry. The results showed that the activities of SOD decreased and the MDA content increased markedly in the hearts of the AM group compared to the control group. H&E staining showed that the control group myocardial cells lined up in order with clear structure and stained equably, while the AM group myocardial cells lined up in disorder with an augmented cell body and the appearance of many granules and interstitial fibrosis. Compared to the control group, in the hearts of the AM group, TSP-1 and TGF-β protein expression in myocardial cells showed a significant increase (P<0.01). TSP-1 and TGF-β expression increased in the myocardium, which may be related to pathological changes of age-related heart diseases, such as hypertrophy, fibrosis of myocardial cells and microvessel dissepiment thickening.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Investigation of thrombospondin-1 and transforming growth factor-β expression in the heart of aging mice

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