Comparative proteomic analysis of the function and network mechanisms of MASPIN in human lung cells
- Authors:
- Yao Liu
- Yi Geng
- Kuanzhi Li
- Fang Wang
- Haiping Zhou
- Wanhu Wang
- Jie Hou
- Wenchao Liu
View Affiliations
Affiliations: Baoji Center Hospital of Shanxi Province, Baoji, Shanxi 721008, P.R. China, Department of Clinical Oncology, State Key Discipline of Cell Biology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, P.R. China
- Published online on: December 22, 2011 https://doi.org/10.3892/etm.2011.427
-
Pages:
470-474
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
MASPIN, which is also known as Serpin B5, is a novel tumor suppressor. Emerging evidence suggests that MASPIN acts as a multifaceted protein in various types of cancer, including prostate, breast and pancreatic cancer. It interacts with diverse groups of intercellular and extracellular proteins, regulating cell adhesion, motility, apoptosis and angiogenesis, and is involved in mammary gland development. As MASPIN is a multifunctional factor in cancer pathways, its function remains poorly illuminated. In this study, we compared the protein profiles of LC5 cell lines with MASPIN overexpression and knockdown using comparative two-dimensional gel electrophoresis. The differences in protein expression, visualized as differences in spots, were identified by time‑of‑flight (TOF)/TOF mass spectometry. Significant differences were observed between overexpressing and knocked down cells, including eight spots that were unique and sixteen spots that were up- or down-regulated by more than 4-fold. Six genes, including Sdccag8, Ldoc1, SCAI, SDCCAG3, CT62 and NEDD9 were unique in MASPIN‑expressing cell lines, but absent in knock-out cell lines, in which most of them play a significant role in the invasion of cancer cells. Moreover, the Brms1 and CAGE1 genes were identified as being uniquely expressed in knocked down cell lines, which were associated with the development and progression of tumors. The data from this study shed some light on the function, as well as the general network mechanisms of MASPIN in lung cancer.
View References
|
1.
|
Z ZouC GaoAK Nagaichp53 regulates the
expression of the tumor suppressor gene maspinJ Biol
Chem27560516054200010.1074/jbc.275.9.605110692390
|
|
2.
|
HY ShiW ZhangR LiangModeling human breast
cancer metastasis in mice: maspin as a paradigmHistol
Histopathol18201206200312507299
|
|
3.
|
S AbrahamW ZhangN GreenbergM ZhangMaspin
functions as tumor suppressor by increasing cell adhesion to
extracellular matrix in prostate tumor cellsJ
Urol16911571161200310.1097/01.ju.0000040245.70349.3712576872
|
|
4.
|
S MachtensJ SerthC BokemeyerExpression of
the p53 and Maspin protein in primary prostate cancer: correlation
with clinical featuresInt J
Cancer95337342200110.1002/1097-0215(20010920)95:5%3C337::AID-IJC1059%3E3.0.CO;2-111494236
|
|
5.
|
JS SchaeferM ZhangRole of maspin in tumor
metastasis and angiogenesisCurr Mol
Med3653658200310.2174/156652403347951914601639
|
|
6.
|
ML CherHR Biliran JrS BhagatMaspin
expression inhibits osteolysis, tumor growth, and angiogenesis in a
model of prostate cancer bone metastasisProc Natl Acad Sci
USA10078477852200310.1073/pnas.133136010012788977
|
|
7.
|
A DokrasJ CoffinL Field6116172006
|
|
8.
|
DT BrandtC BaarlinkTM KitzingSCAI acts as
a suppressor of cancer cell invasion through the transcriptional
control of beta1-integrinNat Cell
Biol11557568200910.1038/ncb186219350017
|
|
9.
|
N NeznanovL NeznanovaB AngresAV
GudkovSerologically defined colon cancer antigen 3 is necessary for
the presentation of TNF receptor 1 on cell surfaceDNA Cell
Biol24777785200510.1089/dna.2005.24.77716332174
|
|
10.
|
K NagasakiC SchemC von
KaisenbergLeucine-zipper protein, LDOC1, inhibits NF-kappaB
activation and sensitizes pancreatic cancer cells to apoptosisInt J
Cancer105454458200310.1002/ijc.1112212712434
|
|
11.
|
M CicekR FukuyamaDR WelchN SizemoreG
CaseyBreast cancer metastasis suppressor 1 inhibits gene expression
by targeting nuclear factor-kappaB activityCancer
Res6535863595200510.1158/0008-5472.CAN-04-313915867352
|
|
12.
|
S ParkY LimD LeeIdentification and
characterization of a novel cancer/testis antigen gene
CAGE-1Biochim Biophys
Acta1625173182200310.1016/S0167-4781(02)00620-612531476
|
|
13.
|
M KimJD GansC NogueiraComparative
oncogenomics identifies NEDD9 as a melanoma metastasis
geneCell12512691281200610.1016/j.cell.2006.06.00816814714
|
|
14.
|
RA MerrillAW SeeML WertheimM
Clagett-DameCrk-associated substrate (Cas) family member, NEDD9, is
regulated in human neuroblastoma cells and in the embryonic
hindbrain by all-trans retinoic acidDev
Dyn231564575200410.1002/dvdy.20159
|
|
15.
|
A BonnefoyK DaenensHB FeysThrombospondin-1
controls vascular platelet recruitment and thrombus adherence in
mice by protecting (sub)endothelial VWF from cleavage by
ADAMTS13Blood107955964200610.1182/blood-2004-12-485616204318
|
|
16.
|
RB LorsbachJ MooreSO AngW SunN LennyJR
DowningRole of RUNX1 in adult hematopoiesis: analysis of
RUNX1-IRES-GFP knock-in mice reveals differential lineage
expressionBlood10325222529200410.1182/blood-2003-07-243914630789
|
|
17.
|
H HiraiIM SamokhvalovT FujimotoS
NishikawaJ ImanishiS NishikawaInvolvement of Runx1 in the
down-regulation of fetal liver kinase-1 expression during
transition of endothelial cells to hematopoietic
cellsBlood10619481955200510.1182/blood-2004-12-487215928041
|
|
18.
|
DD SchlaepferCR HauckDJ SiegSignaling
through focal adhesion kinaseProg Biophys Mol
Biol71435478199910.1016/S0079-6107(98)00052-2
|
|
19.
|
JA Bernard-TrifiloST LimS HouDD
SchlaepferD IlicAnalyzing FAK and Pyk2 in early integrin signaling
eventsCurr Protoc Cell Biol Chapter 14: Unit 1472006
|
|
20.
|
N MaassM TeffnerF RoselDecline in the
expression of the serine proteinase inhibitor maspin is associated
with tumour progression in ductal carcinomas of the breastJ
Pathol195321326200110.1002/path.94811673829
|
|
21.
|
Y UmekitaY OhiY SagaraH YoshidaExpression
of maspin predicts poor prognosis in breast-cancer patientsInt J
Cancer100452455200210.1002/ijc.1050012115529
|
|
22.
|
BW FutscherMM OshiroRJ WozniakRole for DNA
methylation in the control of cell type specific maspin
expressionNat Genet31175179200210.1038/ng88612021783
|
|
23.
|
N MaassM BiallekF RoselHypermethylation
and histone deacetylation lead to silencing of the maspin gene in
human breast cancerBiochem Biophys Res
Commun297125128200210.1016/S0006-291X(02)02136-812220518
|
|
24.
|
R BassAM FernandezV EllisMaspin inhibits
cell migration in the absence of protease inhibitory activityJ Biol
Chem2774684546848200210.1074/jbc.C20053220012384513
|
|
25.
|
H Biliran JrS ShengPleiotrophic inhibition
of pericellular urokinase-type plasminogen activator system by
endogenous tumor suppressive maspinCancer
Res6186768682200111751384
|
|
26.
|
N JiangY MengS ZhangE Mensah-OsmanS
ShengMaspin sensitizes breast carcinoma cells to induced
apoptosisOncogene2140894098200210.1038/sj.onc.120550712037665
|
|
27.
|
HY ShiR LiangNS TempletonM ZhangInhibition
of breast tumor progression by systemic delivery of the maspin gene
in a syngeneic tumor modelMol
Ther5755761200210.1006/mthe.2002.060212027560
|
