Clinical significance of radiation-induced CD133 expression in residual rectal cancer cells after chemoradiotherapy

Kawamoto,Aya; Tanaka,Koji; Saigusa,Susumu; Toiyama,Yuji; Morimoto,Yuhki; Fujikawa,Hiroyuki; Iwata,Takashi; Matsushita,Kohei; Yokoe,Takeshi; Yasuda,Hiromi; Inoue,Yasuhiro; Miki,Chikao; Kusunoki,Masato

doi:10.3892/etm.2011.438

March 2012 Volume 3 Issue 3

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March 2012 Volume 3 Issue 3

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Article

Clinical significance of radiation-induced CD133 expression in residual rectal cancer cells after chemoradiotherapy

Authors:
- Aya Kawamoto
- Koji Tanaka
- Susumu Saigusa
- Yuji Toiyama
- Yuhki Morimoto
- Hiroyuki Fujikawa
- Takashi Iwata
- Kohei Matsushita
- Takeshi Yokoe
- Hiromi Yasuda
- Yasuhiro Inoue
- Chikao Miki
- Masato Kusunoki
View Affiliations / Copyright

Affiliations: Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Mie 514-8507, Japan
Pages: 403-409
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Published online on: December 28, 2011

https://doi.org/10.3892/etm.2011.438
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Abstract

CD133 and CD44 have been considered as markers for colorectal cancer stem cells (CSCs). The association of CD133 and CD44 expression with radiation has not been fully examined in rectal cancer. Both CD133 (PROM) and CD44 mRNA levels were measured in post-chemoradiotherapy (CRT) specimens of 52 rectal cancer patients using real-time RT-PCR and compared to clinicopathological variables and clinical outcome. Their protein levels were examined in the radiation-treated HT29 human colon cancer cell line. Post-CRT CD133 in residual cancer cells was significantly higher than matched pre-CRT CD133 in biopsy specimens (n=30). By contrast, CD44 was significantly lower in post-CRT specimens (P<0.01). CD133 was associated with distant recurrence after CRT followed by surgery (P<0.05). Patients with elevated CD133 in residual cancer cells showed poor disease-free survival (P<0.05). No significant association between post-CRT CD44 and clinical outcome was found. The in vitro study showed that CD133 protein was increased in a radiation dose-dependent manner, despite of the decreased number of clonogenic radiation-surviving cells. CD44 protein was decreased after irradiation. CD133, but not CD44, was increased in radiation-resistant surviving colon cancer cells. Post-CRT CD133 in residual cancer cells may predict metachronous distant recurrence and poor survival of rectal cancer patients after CRT.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Clinical significance of radiation-induced CD133 expression in residual rectal cancer cells after chemoradiotherapy

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