LNA real-time PCR probe quantification of hepatitis B virus DNA
- Authors:
- Qing Wang
- Xueqian Wang
- Junhua Zhang
- Guanghui Song
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Affiliations: Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, P.R. China, Qingdao Research Institute for Population and Family Planning, Qingdao 266003, P.R. China, AI DE Diagnostic Co., Ltd., Qingdao 266003, P.R. China
- Published online on: December 28, 2011 https://doi.org/10.3892/etm.2011.442
-
Pages:
503-508
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Abstract
In the present study, we standardized a TaqMan locked nucleic acid (LNA) real-time polymerase chain reaction (PCR) probe for the accurate quantification and detection of hepatitis B virus (HBV) DNA in serum (plasma), and evaluated its methodology. LNA probe technology had a much better detection performance in HBV DNA than the common TaqMan probe. The assay based on the LNA probe had a wider linear detection range, higher sensitivity, stability and amplification efficiency, and a lower concentration of probes than the TaqMan probe. Among the 15 cases with chronic hepatitis B surface antigen (HBsAg) (+) alone, only 4 cases that were detected by TaqMan real-time PCR were negative; however, the same samples were positive by LNA real-time PCR (p<0.05). A positive correlation between viral load measurements for the 35 samples with HBV-positive DNA was detected in both LNA and TaqMan real-time PCR.
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